Assessment of a 18F-Phenylboronic Acid Radiotracer for Imaging Boron in Maize

Housh, Alexandra Bauer; Matthes, Michaela; Gerheart, Amber; Wilder, Stacy L.; Kil, Kun-Eek; Schueller, Michael; Guthrie, John; McSteen, Paula; Ferrieri, Richard A.

doi:10.3390/ijms21030976

Cited by 13 publications

(22 citation statements)

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“…In a later study, Cd uptake and translocation in 6 rice cultivars of differing Cd affinity (3 high and 3 low uptake cultivars) was investigated using PETIS and autoradiography ( Figure 8B ). 97 Cd uptake and translocation dynamics were observed between high and low affinity varieties, with high-affinity varieties demonstrating more rapid translocation of Cd from roots to shoots and panicles relative to low-affinity varieties. High-affinity varieties also showed a decrease of Cd in roots after peaking, whereas low-affinity varieties reached a plateau, corresponding with greater export elsewhere in the plant.…”

Section: Positron Imaging For Visualization Of Contaminant Dynamics Imentioning

confidence: 95%

From the Outside in: An Overview of Positron Imaging of Plant and Soil Processes

et al. 2020

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Positron-emitting nuclides have long been used as imaging agents in medical science to spatially trace processes non-invasively, allowing for real-time molecular imaging using low tracer concentrations. This ability to non-destructively visualize processes in real time also makes positron imaging uniquely suitable for probing various processes in plants and porous environmental media, such as soils and sediments. Here, we provide an overview of historical and current applications of positron imaging in environmental research. We highlight plant physiological research, where positron imaging has been used extensively to image dynamics of macronutrients, signalling molecules, trace elements, and contaminant metals under various conditions and perturbations. We describe how positron imaging is used in porous soils and sediments to visualize transport, flow, and microbial metabolic processes. We also address the interface between positron imaging and other imaging approaches, and present accompanying chemical analysis of labelled compounds for reviewed topics, highlighting the bridge between positron imaging and complementary techniques across scales. Finally, we discuss possible future applications of positron imaging and its potential as a nexus of interdisciplinary biogeochemical research.

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Section: Positron Imaging For Visualization Of Contaminant Dynamics Imentioning

confidence: 95%

From the Outside in: An Overview of Positron Imaging of Plant and Soil Processes

et al. 2020

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“…The recent application of ablation laser-ICP-MS (inductively coupled plasma mass spectrometry) allowed the development of a mathematical model that enabled the analysis of B distribution along roots of A. thaliana at an extremely high resolution ( Shimotohno et al, 2015 ). To overcome the challenges of B detection in different plant tissues, the fluorinated-18 4-fluorophenylboronic acid radiotracer approach ([18F] FPBA) was recently used ( Housh et al, 2020 ). The images obtained by autoradiography provided insightful information on the patterns of static B location in both root and shoot tissues ( Housh et al, 2020 ), revealing different locations of B distribution.…”

Section: Boron Translocation and Distributionmentioning

confidence: 99%

“…To overcome the challenges of B detection in different plant tissues, the fluorinated-18 4-fluorophenylboronic acid radiotracer approach ([18F] FPBA) was recently used ( Housh et al, 2020 ). The images obtained by autoradiography provided insightful information on the patterns of static B location in both root and shoot tissues ( Housh et al, 2020 ), revealing different locations of B distribution. Briefly, both the root tip and the stretching zone are the ones that exhibited the most intense sign of the tracer with a region between the two devoid of any sign, showing a clear demand for B in these regions ( Housh et al, 2020 ).…”

Section: Boron Translocation and Distributionmentioning

confidence: 99%

“…The images obtained by autoradiography provided insightful information on the patterns of static B location in both root and shoot tissues ( Housh et al, 2020 ), revealing different locations of B distribution. Briefly, both the root tip and the stretching zone are the ones that exhibited the most intense sign of the tracer with a region between the two devoid of any sign, showing a clear demand for B in these regions ( Housh et al, 2020 ). Consequently, future studies using such tools are important to determine element distribution throughout the plant and behavior of specific tissue levels.…”

Section: Boron Translocation and Distributionmentioning

confidence: 99%

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Boron: More Than an Essential Element for Land Plants?

et al. 2021

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Although boron (B) is an element that has long been assumed to be an essential plant micronutrient, this assumption has been recently questioned. Cumulative evidence has demonstrated that the players associated with B uptake and translocation by plant roots include a sophisticated set of proteins used to cope with B levels in the soil solution. Here, we summarize compelling evidence supporting the essential role of B in mediating plant developmental programs. Overall, most plant species studied to date have exhibited specific B transporters with tight genetic coordination in response to B levels in the soil. These transporters can uptake B from the soil, which is a highly uncommon occurrence for toxic elements. Moreover, the current tools available to determine B levels cannot precisely determine B translocation dynamics. We posit that B plays a key role in plant metabolic activities. Its importance in the regulation of development of the root and shoot meristem is associated with plant developmental phase transitions, which are crucial processes in the completion of their life cycle. We provide further evidence that plants need to acquire sufficient amounts of B while protecting themselves from its toxic effects. Thus, the development of in vitro and in vivo approaches is required to accurately determine B levels, and subsequently, to define unambiguously the function of B in terrestrial plants.

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“…Advancing knowledge of B deficiency at the molecular and cellular level is a challenge, partly due to the limited availability of B-imaging techniques. Housh et al [13] have demonstrated that radioactively labelled [ 18 F]4-fluorophenylboronic acid (FPBA), which is a derivative of phenylboronic acid (PBA) that mimics B deficiency, accumulates at the root tip, the elongation zone, and the lateral root initiation sites in maize roots, indicating a demand for B in these regions, and also moves to the shoot where it accumulates along the leaf edges. This report supports that radioactively labelled [ 18 F]FPBA can be used to image sites of B demand at the tissue level.…”

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confidence: 99%