Assessment and characterization of degradation effect for the varied degrees of ultra-violet radiation onto the collagen-bonded polypropylene non-woven fabric surfaces

Tyan, Yu‐Chang; Liao, Jiunn Der; Klauser, Ruth; Wu, Ie-Der; Weng, Chih-Chiang

doi:10.1016/s0142-9612(01)00080-1

Cited by 64 publications

(73 citation statements)

References 23 publications

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“…In order to immobilize 125 I anti-HSA monoclonal antibody, the 11-mercaptoundecanoic acid/Au surface was immersed in the solution containing coupling agents: 75 mM N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, E-6383, Sigma) and 15 mM N-hydroxysuccinimide (NHS, H-7377, Sigma) at 4 °C for 30 min (van Delden et al, 1997;Kuijpers et al, 2000). Water-soluble EDC and NHS were used to activate O=C-OH (Kang et al, 1993;Tyan et al, 2002) and then the EDC-NHS solution was replaced by a phosphate buffered saline (PBS, URPBS001, UniRegion Bio-Tech), containing 0.2 μg/mL HSA-antibody at 4 °C for 24 hrs. The SAMs chips were thereafter washed by D.I.…”

Section: Immobilization Of Anti-hsamentioning

confidence: 99%

Quartz crystal microbalance in clinical application

Yang¹,

Jong²,

Chung³

et al. 2011

Biosensors for Health, Environment and Biosecurity

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Section: Immobilization Of Anti-hsamentioning

confidence: 99%

Quartz crystal microbalance in clinical application

Yang¹,

Jong²,

Chung³

et al. 2011

Biosensors for Health, Environment and Biosecurity

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“…Both, the ionized species and the free radicals in plasma may interact with molecular chains, which are correlated with the reactivity of fluc tuated non-equilibrium states of plasma species with the substrate. Previous studies have shown that plasma density is a substantial factor to create thermal decom posable hydro-peroxide at polymeric support [12,13]. Plasma pre-treated fabrics are further grafted with hydrophilic monomer to arise long-term hydrophilicity on the fabric surfaces.…”

Section: Biomedical Engineering-applications Basis and Communications 21mentioning

confidence: 99%

“…Plasma pre-treated fabrics are further grafted with hydrophilic monomer to arise long-term hydrophilicity on the fabric surfaces. A variety of studies have used acrylic acid (AAc) as the monomer to graft copolymerization with the plasma-treated substrate and to form a steadily hydrophilic surface [12][13][14]. This work aims to promote pAAc-grafting density and to bond with collagen for wound healing base substrates and tissue regeneration related applications.…”

Section: Biomedical Engineering-applications Basis and Communications 21mentioning

confidence: 99%

“…Collagen immobilization process and subse quently the effectiveness of surface-immobilized col lagen are correlated with the hydrophilic property at the surface of a bulk support [4][5][6][7]13]. Commonly, covalent binding of reactive groups is adequate as hydro philic and functional surface through reactive supports or spacer arms, which also exhibit improved bioactivity [ 15].…”

Section: Biomedical Engineering-applications Basis and Communications 21mentioning

confidence: 99%

“…To investi gate the bioactivity of immobilized collagen on porous surfaces, selected biological tests, e.g., aPTT (acti vated partial thromhoplastin time), TT (thrombin time), fibrinogen concentration and platelet adhesion tests, have been applied. The use of such blood-contact methods is introduced elsewhere [13]. The interpreta tion, however, depends upon the correlated clotting factors applied.…”

Section: Biomedical Engineering-applications Basis and Communications 21mentioning

confidence: 99%

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Bioactive Assessment and Bacteria Test for the Varied Degrees of Ultra-Violet Radiation Onto the Collagen-Immobilized Polypropylene Non-Woven Fabric

Liao

Tyan

2002

Biomed. Eng. Appl. Basis Commun.

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Exposure to ultra-violet (UV)-C radiation is a frequently used method to prevent bacteria from invasion of blood-contact biomedical products. Potential damage induced by UV radiation to collagen is of concern due to the decay of bioactivity, considerably correlated with structural alterations. Current investigation indicates to the collagen-immobilized non-woven polypropylene (PP) fabrics with sample temperature ca. 4 °C; the samples are then exposed to UV-254 nm radiation for different time intervals. Using Fourier-Transformed Infrared with Attenuated Total Reflection (FTIR-ATR) and XPS (X-ray Photoelectron Spectroscopy), we examine the chemical structures of samples with different treatments. Blood-clotting effects on the modified samples are assessed by activated partial thromboplastin time, thrombin time, and fibrinogen concentration tests. By means of cell counter and Scanning Electron Microscopy we count red blood cells and platelets adhesion in the modified porous matrix. Applying standard plate count for bacteria tests, E. coli, Bacillus stearothermophilus, Staph. aureus, P. aeruginosa, and Candida albieans are applied. For human plasma incubated samples of various intervals of UV-254 nm radiation, fibrinogen concentration decreases in human plasma, while platelets and red blood cells adhesions increase before UV radiation. The required time for thrombination shows significant change for UV exposure of less than 20 hrs (α = 0.05). Surface analyses indicate that the decrease of R-COOH (derivated from grafted-pAAc or decarboxylation of collagen), amides degradation (broken–NH), and phenylalanine scission (terminated by −OH, tyrosine formation) may gradually damage collagen by increasing the intervals of UV radiation. The XPS measurements of C 1s core levels at 288.1 eV (O=C-NH) and at 289.3 eV (O=C-O) illustrate significant decreases of intensity after radiation time ca. 44 hrs. It is clear that UV-254 nm radiation exposure for ca. 20 hrs has the potential impact to moderate the bioactivities of collagen and therefore act as a vital factor to accelerate bio-degradation. Bacteria test also supports that around 20 hrs of UV radiation, no bacteria clone formation is found on the immobilized collagen. However, the relation between eventual bioactivity of immobilized collagen after UV radiation and the capability of bacteria proliferation should be measured.

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Reagentless Protein Assembly Triggered by Localized Electrical Signals

et al. 2009

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There is great interest in coupling the capabilities of electronics with the molecular-recognition properties of biology to generate hand-held devices that can diagnose diseases at the point-of-care, analyze environmental samples in the field, and assess food safety from the farm to the table. A key challenge is the integration of the labile biological recognition elements (e.g., proteins) at specific device addresses. Here, we report a simple, safe, and generic approach for assembling proteins in response to electrodeimposed electrical signals. This approach relies on the aminopolysaccharide chitosan that can be electrodeposited in response to cathodic signals and then electrochemically activated by anodic signals. The electodeposited and electroactivated chitosan films react with proteins to assemble them with spatial-selectivity and quantitative-control. The evidence presented indicates that the assembled proteins retain their native structure and biological functions. This method for on-demand biofunctionalization of individual electrode addresses should offer a generic approach to assemble proteins for multiplexed analysis.There has been considerable effort to discover mechanisms in which electrode-imposed electrical signals can promote the spatially-selective assembly of proteins at specific addresses. [1,2]

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Assessment and characterization of degradation effect for the varied degrees of ultra-violet radiation onto the collagen-bonded polypropylene non-woven fabric surfaces

Cited by 64 publications

References 23 publications

Quartz crystal microbalance in clinical application

Quartz crystal microbalance in clinical application

Bioactive Assessment and Bacteria Test for the Varied Degrees of Ultra-Violet Radiation Onto the Collagen-Immobilized Polypropylene Non-Woven Fabric

Reagentless Protein Assembly Triggered by Localized Electrical Signals

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