Assessing Travel Conditions: Environmental and Host Influences on Bacterial Surface Motility

Mattingly, Anne E.; Weaver, Abigail A.; Dimkovikj, Aleksandar; Shrout, Joshua D.

doi:10.1128/jb.00014-18

Cited by 45 publications

(64 citation statements)

References 159 publications

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“…Important non-flagellated bacterial movements such twitching occur via retraction of fimbria type IV, particularly in aggregated cells that are highly aligned in close cell-cell contact to form a lattice-like structure, where the cells in the first movement go forward and towards other cells. They touch their own poles, then quickly fit into an aligned position again, which explains the featured spasmodic movement observed in this form of motility [8,17]. This maneuver phenomenon is related to the way bacteria tend to translocate in low water environments and to colonize hydrated surfaces as opposed to free living in fluids [16].…”

Section: Discussionmentioning

confidence: 92%

“…The movement on the surfaces plays an important role in the colonization of flagellated bacteria [8]. Furthermore, it is known that non-flagellated bacteria can perform surface movement through different mechanisms such as twitching, sliding, and gliding [17]. In sliding movement, the expansion of the colony occurs through the production of surfactants which reduce the surface tension [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Bacteria employ mechanisms through pili retraction, such as type IV fimbria, called Twitching motility [8]. Another condition is the bacterial gliding, which is known as a smooth movement usually along the axis of the cell [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Novel non-flagellated surface motility mediated by chemical signaling in Citrobacter rodentium

Melchior

Moreira

2019

Braz J Microbiol

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Enterohemorrhagic (EHEC) and enteropathogenic Escherichia coli (EPEC) are human intestinal pathogens of clinical importance and their mechanism of pathogenicity is widely studied. However, both EHEC and EPEC poorly infect mice, whereas they do not develop important characteristics of the disease, hindering studies about mechanisms of virulence in vivo. Citrobacter rodentium exhibits high similarity of its genes with these human pathogens, including the island of pathogenicity Locus of Enterocyte Effacement (LEE). Therefore, C. rodentium becomes an alternative in vivo model for microorganisms that harbor LEE. The QseC directly regulates LEE as well as virulence mechanisms on these pathogens. Here, we report a novel surface motility in C. rodentium QseC-mediated in this non-flagellated bacterium. Moreover, we show norepinephrine and ethanolamine act as environmental signals in this movement. Hence, this study clarifies a novel role of the sensor QseC in completely unreported motility process of C. rodentium.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Novel non-flagellated surface motility mediated by chemical signaling in Citrobacter rodentium

Melchior

Moreira

2019

Braz J Microbiol

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“…Twitching motility could depend on many factors including surface properties, pili arrangement on bacterial surface, and environmental conditions such as oxygen concentration and fluid flow rate 16 . For example, when pili emanate only at the poles of bacteria (e.g., Pseudomonas aeruginosa ), the bacteria will have persistent motion 17,18 .…”

Section: Introductionmentioning

confidence: 99%

Modelling bacterial twitching in fluid flows: a CFD-DEM approach

Jayathilake

Zuliani

et al. 2019

Preprint

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Bacterial habitats are often associated with fluid flow environments. There is a lack of models 10 of the twitching motility of bacteria in shear flows. In this work, a three-dimensional modelling 11 approach of Computational Fluid Dynamics (CFD) coupled with the Discrete Element Method 12 (DEM) is proposed to study bacterial twitching on flat and groove surfaces under shear flow 13 conditions. Rod-shaped bacteria are modelled as groups of spherical particles and Type IV pili 14 attached to bacteria are modelled as dynamic springs which can elongate, retract, attach and 15 detach. The CFD-DEM model of rod-shape bacteria is validated against orbiting of immotile 16 bacteria in shear flows. The effects of fluid flow rate and surface topography on twitching 17 motility are studied. The model can successfully predict upstream twitching motility of rod-18 shaped bacteria in shear flows. Our model can predict that there would be an optimal range of 19 wall shear stress in which bacterial upstream twitching is most efficient. The results also 20 indicate that when bacteria twitch on groove surfaces, they are likely to accumulate around the 21 downstream side of the groove walls.22 23 24 25 29the surface using appendages called type IV pili (TFP) 2-5 to "explore" the substratum to find 30 suitable sites for growth and thus biofilm formation. Pili emanate from bacterial surface and 31 † The author is currently affiliated with the Department of Oncology, University of Oxford, UK.2 they can be up to several µm long (though they are nm in diameter 6 ). Bacterial twitching 32 occurs through cycles of polymerization and de-polymerization of type IV pili 7,8 . 33Polymerization causes the pilus to elongate and eventually attaching into surfaces. De-34 polymerization makes the pilus to retract and detaching from the surfaces. Pili retraction 35 produces pulling forces on the bacterium, which will be pulled in the direction of the vector 36 sum of the pili forces, resulting in a jerky movement (Figure 1). A typical TFP can produce a 37 force exceeding 100 pN 9 and then a bundle of pili can produce pulling forces up to several nN 38 10 . Bacteria may use pili not only for twitching but also for cell-cell interactions 11,12 , surface 39 sensing 13,14 and DNA uptake 15 . 40Twitching motility could depend on many factors including surface properties, pili 41 arrangement on bacterial surface, and environmental conditions such as oxygen concentration 42 and fluid flow rate 16 . For example, when pili emanate only at the poles of bacteria (e.g., 43Pseudomonas aeruginosa), the bacteria will have persistent motion 17,18 . But, if pili are all 44 around the cell body (e.g., Neisseria gonorrhoeae), the bacteria will have trapped or diffusive 45 motion due to the tug of war mechanism 19,20 . If a pilus detaches while all the pili are in high 46 tension and anti-parallel configuration, the bacterium will suddenly align along the resultant 47 direction of the remaining bounded-pili causing a sudden change of the twitching direction. 48This is ...

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“…Delekta et al show how host lipoproteins can serve as a source of fatty acids for Staphylococcus aureus (27). Mattingly et al review our current understanding of how different nutrient cues promote specific surface motility of bacteria, which is important to understanding of the onset of colonization and infection for motile microbes (28).…”

mentioning

confidence: 99%

24th Annual Midwest Microbial Pathogenesis Conference

Champion

Shrout

2018

J Bacteriol

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The 24th Annual Midwest Microbial Pathogenesis Conference (MMPC) was held at the University of Notre Dame from August 25-27, 2017. The conference provided an opportunity for scientists from the Midwest to discuss new advances in microbial pathogenesis, including how pathogens promote disease, and how they interact with each other, the microbiome and the host. This commentary highlights the MMPC history, the topics presented at the conference and the reports in this issue.

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Assessing Travel Conditions: Environmental and Host Influences on Bacterial Surface Motility

Cited by 45 publications

References 159 publications

Novel non-flagellated surface motility mediated by chemical signaling in Citrobacter rodentium

Novel non-flagellated surface motility mediated by chemical signaling in Citrobacter rodentium

Modelling bacterial twitching in fluid flows: a CFD-DEM approach

24th Annual Midwest Microbial Pathogenesis Conference

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