Assessing the Utility of Isozyme Number for Determining Ploidal Level

Soltis, Douglas E.

doi:10.5642/aliso.19891202.04

Cited by 17 publications

(16 citation statements)

References 20 publications

(36 reference statements)

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“…Similarly, alleles at each locus were numbered in order of decreasing mobility. This nomenclature is concordant with recent locus/allele designations for isozymes of H. annuus (e.g., Rieseberg et al 1991;Rieseberg and Seiler 1990;Rieseberg and Soltis 1989) but is inverted relative to locus/allele designations by earlier authors (Torres 1975;Kahler and Lay 1985).…”

Section: Isozyme Electrophoresissupporting

confidence: 56%

Allozyme variation in domesticated annual sunflower and its wild relatives

et al. 1997

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The annual sunflower (Helianthus annuus L.) is a morphologically and genetically variable species composed of wild, weedy, and domesticated forms that are used for ornament, oilseed, and edible seeds. In this study, we evaluated genetic variation in 146 germplasm accessions of wild and domesticated sunflowers using allozyme analysis. Results from this survey showed that wild sunflower exhibits geographically structured genetic variation, as samples from the Great Plains region of the central United States were genetically divergent from accessions from California and the southwestern United States. Sunflower populations from the Great Plains harbored greater allelic diversity than did wild sunflower from the western United States. Comparison of genetic variability in wild and domesticated sunflower by principal coordinate analysis showed these groups to be genetically divergent, in large part due to differences in the frequency of common alleles. Neighbor-Joining analyses of domesticated H. annuus, wild H. annuus and two closely related wild species (H. argophyllus T. & G. and H. petiolaris Nutt.) showed that domesticated sunflowers form a genetically coherent group and that wild sunflowers from the Great Plains may include the most likely progenitor of domesticated sunflowers. Keywords Helianthus annuus, Allozymes, Crop evolution, Genetic diversity Disciplines Botany | Genetics | Plant Breeding and Genetics CommentsThis article is from Theoretical and Applied Genetics 95 (1997): 532, doi:10.1007/s001220050594. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

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Section: Isozyme Electrophoresissupporting

confidence: 56%

Allozyme variation in domesticated annual sunflower and its wild relatives

et al. 1997

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“…The isozymes were resolved on 12% horizontal starch gel; the buffer systems and staining methods are described in Carrera and Poverene (1995), after Soltis et al (1983). The number of loci and alleles were interpreted according to Torres (1983), Kahler and Lay (1985), Rieseberg and Soltis (1989), and Carrera andPoverene (1991, 1995). Loci were designated by giving the number 1 to the most anodally migrating isozyme and sequentially numbering the additional loci in a decreasing order of electrophoretic mobility.…”

Section: Isozyme Electrophoresismentioning

confidence: 99%

Variability among inbred lines and RFLP mapping of sunflower isozymes

et al. 2002

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Eight isozyme systems were used in this study: acid phosphatase (ACP), alcohol dehydrogenase (ADH), esterase (EST), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), 6-phosphogluconate dehydrogenase (PGD), and phosphoglucomutase (PGM). The polymorphism of these enzyme systems was studied in 25 elite inbred lines. A total of 19 loci were identified, but only eight of them were polymorphic in the germplasm tested. The polymorphic index for the eight informative markers ranged from 0.08 to 0.57, with a mean value of 0.36. Five isozyme loci were mapped in F 2:3 populations with existing RFLP data. Est-1, Gdh-2 and Pgi-2 were mapped to linkage groups 3, 14 and 9, respectively. As in previous reports, an ACP locus and a PGD locus were found to be linked, both located in linkage group 2 of the public sunflower map.

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“…The detailed methods of electrophoresis and staining of enzymes we employed have been described elsewhere (Soltis et al 1983;Rieseberg and Soltis 1988;Rieseberg, Soltis, and Soltis 1988). The following 12 enzymes were analyzed: acid phosphatase (APH), alcohol dehydrogenase (ADH), aldolase (ALD), glutamate dehydrogenase (GDH), glyceraldehyde-3-phosphate dehydrogenase ([NADP]/ G3PDH), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase (6-PGD), shikimate dehydrogenase (SKDH), and triosephosphate isomerase (TPI).…”

Section: Electrophoresismentioning

confidence: 99%

“…The following 12 enzymes were analyzed: acid phosphatase (APH), alcohol dehydrogenase (ADH), aldolase (ALD), glutamate dehydrogenase (GDH), glyceraldehyde-3-phosphate dehydrogenase ([NADP]/ G3PDH), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase (6-PGD), shikimate dehydrogenase (SKDH), and triosephosphate isomerase (TPI). Enzymes were resolved on 12% starch gels using the following buffer systems from Soltis et al (1983): for ADH, GDH, and TPI, system 6; for PGI, ME, and ALD, a modification of system 8 (Rieseberg and Soltis 1987); for PGM, 6-PGD, and SKDH, system 9; and for [NADP]G3PDH, IDH, and APH, system 1. Loci were designated sequentially with the most anodally migrating isozyme designated 1, the next 2, and so on.…”

Section: Electrophoresismentioning

confidence: 99%

“…Such information has been used to: 1) describe levels of genetic variation within and among populations or species (Nevo 1978;Brown 1979;Hamrick, Linhart, and Mitton 1979;Karron 1987); 2) examine how genetic variation is distributed (Nevo 1978;Loveless and Hamrick 1984); 3) examine relationships between closely related species (Gottlieb 1981;Crawford 1983); and 4) detect hybridization and introgression phenomena (Selander, Hunt, and Yang 1969;Levin 1975;Rieseberg, Soltis, and Palmer 1988a). Although the significance of electrophoretic evidence for studies of rare and endangered taxa has long been recognized, only recently has a concerted effort been made to apply allozyme data to questions in conservation biology (Chambers and Bayless 1983;Crawford, Stuessy, and Silva 0.…”

Section: Introductionmentioning

confidence: 99%

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Allozyme Variation in Hellianthus Praecox Ssp. Hirtus, A Rare Sunflower from Southern Texas

Reiseberg¹,

Doyle²

1989

aliso

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Allozyme data were used to evaluate the genetic status of a rare endemic sunflower from Texas, Helianthus praecox ssp. hirtus. Comprising approximately 200 plants, the taxon is presently known from a single population in the Carrizo Springs region of Southern Texas. Electrophoretic examination of I 7 enzyme loci revealed that ssp. hirtus is genetically similar to, but distinct from, the two more widespread subspecies of H. praecox: ssp. praecox and ssp. runyonii. Levels of genetic variation in the single population of ssp. hirtus (P = 0.4 7, A = 1.88, and H = 0.17) are similar to those observed for other populations in the H. praecox complex (P = 0.44, A = 1.68, and H = 0.15). Thus, if isozyme loci are used as the one indicator of genetic variation in ssp. hirtus, it cannot be concluded that this taxon is endangered due to a loss of genetic variation. Nevertheless, due to the highly endemic status of ssp. hirtus serious conservation efforts must be made to ensure the long-term preservation of this taxon. Several management recommendations are given herein.

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Assessing the Utility of Isozyme Number for Determining Ploidal Level

Cited by 17 publications

References 20 publications

Allozyme variation in domesticated annual sunflower and its wild relatives

Allozyme variation in domesticated annual sunflower and its wild relatives

Variability among inbred lines and RFLP mapping of sunflower isozymes

Allozyme Variation in Hellianthus Praecox Ssp. Hirtus, A Rare Sunflower from Southern Texas

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