Assessing the suitability of Imaging-PAM fluorometry for monitoring growth of benthic diatoms

Stock, Willem; Blommaert, Lander; Daveloose, Ilse; Vyverman, Wim; Sabbe, Koen

doi:10.1016/j.jembe.2019.02.003

Cited by 11 publications

(7 citation statements)

References 33 publications

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“…The interest in using F t instead of the well-known MPB biomass proxy F 0 (Consalvey et al, 2005) was to avoid darkening of the MPB, which is known to induce counteracting downward migration, and would have therefore disrupted rhythmic dynamics in MPB migration. Stock et al (2019) also noticed that F 0 could be successfully replaced by F t under some experimental conditions [see also Ni Longphuirt et al (2006)]. We verified that F t was well correlated to the Chl a content of sediment surface (R 2 = 0.84), a correlation as strong as the ones previously reported between F t (Ni Longphuirt et al, 2006) or F 0 (Honeywill et al, 2002;Serôdio et al, 2006) and Chl a.…”

Section: Monitoring Of the Changes In The Mpb Pab At The Surface Of Ssupporting

confidence: 87%

The Vertical Migratory Rhythm of Intertidal Microphytobenthos in Sediment Depends on the Light Photoperiod, Intensity, and Spectrum: Evidence for a Positive Effect of Blue Wavelengths

et al. 2020

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Barnett et al. Intertidal Microphytobenthos Light-Dependent Rhythmic Migration MPB surface accumulation, as compared to other wavelengths (white, green, and red) in patterns that were intensity-dependent and species-dependent. In particular, we found two species, Navicula spartinetensis and Gyrosigma fasciola, which strongly migrate up under blue light and could potentially be used as model species for further studying the light-responses of intertidal MPB.

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Section: Monitoring Of the Changes In The Mpb Pab At The Surface Of Ssupporting

confidence: 87%

The Vertical Migratory Rhythm of Intertidal Microphytobenthos in Sediment Depends on the Light Photoperiod, Intensity, and Spectrum: Evidence for a Positive Effect of Blue Wavelengths

et al. 2020

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“…Daily biomass estimates were obtained using both pulse amplitude modulation (PAM) fluorometry (IMAGING-PAM M-Series Maxi version, Walz) and cell counts (Stock et al, 2019). Minimal fluorescence (F 0 ) was measured (intensity: 6, gain: 3, and frequency: 1) daily, for a period of 7 days.…”

Section: Methodsmentioning

confidence: 99%

Diatom-Bacteria Interactions Modulate the Composition and Productivity of Benthic Diatom Biofilms

et al. 2019

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Benthic diatoms are dominant primary producers in intertidal mudflats and constitute a major source of organic carbon to consumers and decomposers residing within these ecosystems. They typically form biofilms whose species richness, community composition and productivity can vary in response to environmental drivers and their interactions with other organisms (e.g., grazers). Here, we investigated whether bacteria can affect diatom community composition and vice versa, and how this could influence the biodiversity-productivity relation. Using axenic experimental communities with three common benthic diatoms (Cylindrotheca closterium, Navicula phyllepta, and Seminavis robusta), we observed an increase in algal biomass production in diatom co-cultures in comparison to monocultures. The presence of bacteria decreased the productivity of diatom monocultures while bacteria did not seem to affect the overall productivity of diatoms grown in co-cultures. The effect of bacteria on diatom growth, however, appeared to be species-specific, resulting in compositional shifts when different diatom species were grown together. The effect of the diatoms on the bacteria also proved to be species-specific as each diatom species developed a bacterial community that differed in its composition. Together, our results suggest that interactions between bacteria and diatoms residing in mudflats are a key factor in the structuring of the benthic microbial community composition and the overall functioning of that community.

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“…Cellular fluorescence parameters were monitored using Pulsed-Amplitude-Modulation based fluorometry (AquaPen-C 100; Photon Systems Instruments, Drasov, Czech Republic) after 15 min dark incubation of 2 mL cell suspensions. This method yields the cells' photosynthetic capacity (the quantum yield of Photosystem II) as F v /F m and the darkadapted minimal fluorescence parameter (F 0 ) as a reliable proxy for biomass during the exponential growth phase [49]. Maximum growth rates during batch cultivation were calculated by multiplication of the doubling time k with the natural logarithm of 2, where k was determined by regression of either F 0 values or cell density values during the exponential growth phase according to the equation k = log 2 (N t /N 0 )/∆t [50].…”

Section: Cellular Growth and Photophysiology Measurementsmentioning

confidence: 99%

Potential for Biomass Production and Remediation by Cultivation of the Marine Model Diatom Phaeodactylum tricornutum in Oil Field Produced Wastewater Media

Gillard

Hernandez

Contreras

et al. 2021

Water

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While oilfield produced water (PW) is one of the largest, unclaimed wastewater streams of the oil industry, it could potentially be used as a cultivation medium for microalgae. Microalgae could help with the remediation of this water while also delivering biomass that can be transformed into valuable byproducts such as biofuels. The coupling of these two purposes is expected to cut production costs of biofuels while aiding environmental protection. In this study, we compared the cultivation capacity of the marine model diatom Phaeodactylum tricornutum in media at varying salinities and in media composed of PW from two oilfields in the Central Valley of California that differed drastically in the concentration of inorganic and organic constituents. Specifically, we measured the carrying capacity of these media, the maximum growth rates of P. tricornutum, its cellular lipid accumulation capacity, and its capacity to remediate the most polluted PW source. Our study shows that P. tricornutum can successfully adjust to the tested cultivation media through processes of short-term acclimation and long-term adaptation. Furthermore, the cultivation of P. tricornutum in the most heavily polluted PW source led to significant increases in cell yield and improved photosynthetic capacity during the stationary phase, which could be attributed chiefly to the higher levels of nitrate present in this PW source. Chemical water analyses also demonstrated the capability of P. tricornutum to remediate major nutrient content and potentially harmful elements like fluorine and copper. Because P. tricornutum is amenable to advanced genetic engineering, which could be taken advantage of to improve its cultivation resilience and productivity in an economic setting, we propose this study as a step towards essential follow-up studies that will identify the genetic regulation behind its growth in oilfield PW media and its remediation of the PW constituents.

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Assessing the suitability of Imaging-PAM fluorometry for monitoring growth of benthic diatoms

Cited by 11 publications

References 33 publications

The Vertical Migratory Rhythm of Intertidal Microphytobenthos in Sediment Depends on the Light Photoperiod, Intensity, and Spectrum: Evidence for a Positive Effect of Blue Wavelengths

The Vertical Migratory Rhythm of Intertidal Microphytobenthos in Sediment Depends on the Light Photoperiod, Intensity, and Spectrum: Evidence for a Positive Effect of Blue Wavelengths

Diatom-Bacteria Interactions Modulate the Composition and Productivity of Benthic Diatom Biofilms

Potential for Biomass Production and Remediation by Cultivation of the Marine Model Diatom Phaeodactylum tricornutum in Oil Field Produced Wastewater Media

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