Assessing the Potential to Extrapolate Genetic-Based Restoration Strategies Between Ecologically Similar but Geographically Separate Locations of the Foundation Species Vallisneria americana Michx

Marsden, Brittany W.; Ngeve, Magdalene N.; Engelhardt, Katharina A. M.; Neel, Maile C.

doi:10.1007/s12237-021-01031-z

Cited by 1 publication

(18 citation statements)

References 126 publications

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“…The 135 pre‐storm samples (Marsden et al., 2022) were assigned to 73 MLGs (GD = 0.54) compared to 110 MLGs for 239 post‐storm samples (GD = 0.46). Values of p sex for MLGs that occurred multiple times were always

\ll 0.01

, so replicate occurrences of MLGs were considered to be members of the same clonal lineage.…”

Section: Resultsmentioning

confidence: 99%

“…We extracted genomic DNA from leaf tissue, using Synergy Plant DNA kits (OPS Diagnostics). We used TopTaq DNA Polymerase Kits (Qiagen Inc.) and species-specific primers (Burnett et al, 2009) to perform PCR to amplify the same 10 microsatellite loci using the same PCR conditions as Marsden et al (2022). Initial denaturation of 95°C for 15 min was followed by 34 cycles of 30 s denaturation, 90 s of annealing at a temperature of 57°C and elongation of 80 s at 72°C.…”

Section: Dna Extraction Amplification and Fragment Analysesmentioning

confidence: 99%

“…DNA Analyzer with GeneScan™-500 using a 500 LIZ™ size standard (Applied Biosystems/ThermoFisher Scientific). Peak data were analyzed using GeneMapper v3.7 (Applied Biosystems) and all allele calls were inspected and made consistent with previously analyzed data following standards and decision rules of Lloyd et al (2011) and Marsden et al (2022). Ambiguous calls were re-genotyped and if the call remained ambiguous after 3-4 attempts, the alleles were coded as missing.…”

Section: Dna Extraction Amplification and Fragment Analysesmentioning

confidence: 99%

“…We knew from prior analysis (Marsden et al, 2022) that three of the five 2011 sites had evidence of population genetic bottlenecks.…”

Section: Genetic Diversitymentioning

confidence: 99%

“…Pre-storm samples indicated seed dispersal between sites separated by up to ~32 km and vegetative dispersal up to ~17 km (Marsden et al, 2022). In the non-tidal Potomac River of Maryland (USA), vegetative dispersal has yielded V. americana clones that extend 50-160 river km (Lloyd et al, 2011), although dispersal likely occurs in a stepping-stone fashion across shorter distances.…”

Section: Introductionmentioning

confidence: 99%

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Calm after the storm? Similar patterns of genetic variation in a riverine foundation species before and after severe disturbance

Ngeve,

Engelhardt,

Gray

et al. 2023

Ecology and Evolution

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In summer 2011, Tropical storms Lee and Irene caused an estimated 90% decline of the submersed aquatic plant Vallisneria americana Michx. (Hydrocharitaceae) in the Hudson River of New York (USA). To understand the genetic impact of such large‐scale demographic losses, we compared diversity at 10 microsatellite loci in 135 samples collected from five sites just before the storms with 239 shoots collected from nine sites 4 years after. Although 80% of beds sampled in 2011 lacked V. americana in 2015, we found similar genotypic and genetic diversity and effective population sizes in pre‐storm versus post‐storm sites. These similarities suggest that despite local extirpations concentrated at the upstream end of the sampling area, V. americana was regionally resistant to genetic losses. Similar geographically based structure among sites in both sampling periods suggested that cryptic local refugia at previously occupied sites facilitated re‐expansion after the storms. However, this apparent resistance to disturbance may lead to a false sense of security. Low effective population sizes and high clonality in both time periods suggest that V. americana beds were already small and had high frequency of asexual reproduction before the storms. Dispersal was not sufficient to recolonize more isolated sites that had been extirpated. Chronic low diversity and reliance on asexual reproduction for persistence can be risky when more frequent and intense storms are paired with ongoing anthropogenic stressors. Monitoring genetic diversity along with extent and abundance of V. americana will give a more complete picture of long‐term potential for resilience.

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\ll 0.01

, so replicate occurrences of MLGs were considered to be members of the same clonal lineage.…”

Section: Resultsmentioning

confidence: 99%

Section: Dna Extraction Amplification and Fragment Analysesmentioning

confidence: 99%

Section: Dna Extraction Amplification and Fragment Analysesmentioning

confidence: 99%

“…We knew from prior analysis (Marsden et al, 2022) that three of the five 2011 sites had evidence of population genetic bottlenecks.…”

Section: Genetic Diversitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Calm after the storm? Similar patterns of genetic variation in a riverine foundation species before and after severe disturbance

Ngeve,

Engelhardt,

Gray

et al. 2023

Ecology and Evolution

Self Cite

View full text Add to dashboard Cite

show abstract

Assessing the Potential to Extrapolate Genetic-Based Restoration Strategies Between Ecologically Similar but Geographically Separate Locations of the Foundation Species Vallisneria americana Michx

Cited by 1 publication

References 126 publications

Calm after the storm? Similar patterns of genetic variation in a riverine foundation species before and after severe disturbance

Calm after the storm? Similar patterns of genetic variation in a riverine foundation species before and after severe disturbance

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