Assessing the potential phytotoxicity of digestate from winery wastes

Abstract

In this study, digestate from winery wastes was investigated focusing on phytotoxicity using macrophytes and evaluating the potential contribution of ammonium and copper. Spreading of digestate on soil could represent a suitable approach to recycle nutrients and organic matter, creating an on site circular economy. In this study, digestate quality was evaluated considering both chemical-physical characteristics and biological toxicity applying germination test. The effluent did not meet the entire amendment quality standard defined by Italian law (Decree 75/2010 germination index > 60% with solution of 30% v/v of digestate), but bio-stimulation was observed at low doses (3.15-6.25% v/v) for S. alba and S. saccharatum. The beneficial concentration agreed with Nitrate Directive dose and suggested that limited addition of digestate could have several positive effects on soil characteristics and on crop growth. Specific test using ammonium and copper solutions showed that these pollutants were not directly correlated to observed phytotoxicity.

Responses of Two Barley Subspecies to In vitro-Induced Heavy Metal Stress: Seeds Germination, Seedlings Growth and Cytotoxicity Assay

“…To date, several nations used the germination index (GI) to evaluate the quality of amendment as the GP -germination percentage; GI -germination index; SL -shoot length; RL -root length; FW -fresh weight; DW -dry weight; MI -mitotic index + Significant at the 0.05 level of probability; ++ Significant at the 0.01 level of probability result of the combination of plant-germination and root elongation (Da Ros et al 2018). GI was preferred for its sensitivity, short time requirement and simplicity, being the phase of germination intensely influenced by environmental conditions (Wang 1991).…”

How Has the Wine Sector Incorporated the Premises of Circular Economy?

“…Number of papers References 2019 8 [8], [37], [38], [39], [40], [41], [42], [43] 2018 23 [3], [7], [31], [44], [45], [46], [47], [48], [49], [50], [51], [52], [53], [54], [55], [56], [57], [58], [59], [60], [61], [62], [ literature review. Fig.…”

Anaerobic Co-digestion of Catering and Agro-Industrial Waste: A Step Forward Toward Waste Biorefinery

“…The phytotoxicity test of both digestate was conducted by using 72 h seed germination assay. Germination assay is a rapid and widely applied method for evaluating phytotoxicity of organic product (Da Ros et al, 2018). Three dilution of each digestate material (post treated and untreated digestate) were prepared using 5, 10, and 20 g of dried digestate in 100 ml of distilled water.…”

“…Phytotoxicity is widely applied and promising parameter to evaluate digestate application on plants which describes the index its overall eco-toxicological impact (Da Ros et al, 2018). The phytotoxicity test of both untreated and post treated digestate at varying digestate solution (5, 10, and 20 g per 100 ml water) was performed using seed germination assay on wheat seeds.…”

“…The digestate effluents carry vital nutrients capable of promoting plant growth (Tornwall et al, 2017). Application of such high nutrient entities to the soil not only ensure the sound disposal of anaerobic byproducts, but also is an appealing solution to declining soil nutrient balance, which has identified as an important soil health problem worldwide (Galvez et al, 2012;Da Ros et al, 2018). However, the characteristic phytotoxicity limits the applications of anaerobic digestate as soil conditioner (Coelho et al, 2018).…”