Assessing the potential of <i>Schizochytrium</i> sp. HX‐308 for microbial lipids production from corn stover hydrolysate

Wang, Ling‐Ru; Zhang, Zixu; Wang, Yu‐Zhou; Li, Zijia; Huang, Pengwei; Sun, Xiao‐Man

doi:10.1002/biot.202100470

Cited by 10 publications

(7 citation statements)

References 38 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The suspension was stirred by a propeller agitator (4) powered by an induction motor (5). The retentate outlet (6) was closed, and the permeate was collected in the tank (7). Permeate flow was measured using a cylinder and stopwatch.…”

Section: Study On Reactor Hydrodynamicsmentioning

confidence: 99%

See 1 more Smart Citation

Hydrodynamic studies of innovative membrane reactor for enzymatic hydrolysis of lignocellulosic waste

Dąbkowska‐Susfał,

Lipińska,

Sobieszuk

et al. 2024

Biotechnology Journal

View full text Add to dashboard Cite

This paper presents the study concerning the impact of the basic operational parameters on the performance of an innovative microfiltration membrane reactor applied for enzymatic hydrolysis of lignocellulosic biomass. The concept and basic hydrodynamics of the reactor with tubular ceramic membranes and a propeller agitator were shown. Besides, the efficiency of enzymatic hydrolysis of corn straw was studied to check reactor functionality. It has been proven that the proposed reactor construction can improve the microfiltration of lignocellulosic suspension by reducing the cake layer on the membrane surface. Increasing the rotational speed of the propeller agitator also improved the filtration efficiency. The permeate flux during the microfiltration experiments was lower for smaller lignocellulose biomass fraction (D < 425 μm) when compared to the less fragmented corn straw (425 < D < 900 μm). For larger solid fractions, a stirring speed increase enhanced the separation efficiency regardless of the differences in biomass concentration. In contrast, this trend for the finer biomass fraction was only noticeable for the highest used biomass concentration (C = 2.0%). Considering the enzymatic hydrolysis of corn straw, membrane separation of reaction products positively influenced the process yield, and the results depended on the applied operational parameters.

show abstract

Section: Study On Reactor Hydrodynamicsmentioning

confidence: 99%

“…Membranes were concentrically placed around the propeller stirrer (2) (diameter, d = 11 cm). A guiding tube (d = 13 cm) is located between the membranes and the propeller(7). The suspension of the feedstock (i.e., grounded lignocellulosic waste) and enzymes in the buffer flow into the reactor by the inlet (4).…”

mentioning

confidence: 99%

Hydrodynamic studies of innovative membrane reactor for enzymatic hydrolysis of lignocellulosic waste

Dąbkowska‐Susfał,

Lipińska,

Sobieszuk

et al. 2024

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…Schizochytrium sp., a heterotrophic marine organism, has been used industrially to produce docosahexaenoic acid (DHA). [1][2][3] The typical condition of laboratory scale cultivation for Schizochytrium sp. is artificial seawater, which requires a large amount of water resources and costs a lot.…”

Section: Introductionmentioning

confidence: 99%

“…Schizochytrium sp., a heterotrophic marine organism, has been used industrially to produce docosahexaenoic acid (DHA) 1–3 . The typical condition of laboratory scale cultivation for Schizochytrium sp.…”

Section: Introductionmentioning

confidence: 99%

Enhancing the biomass and docosahexaenoic acid‐rich lipid accumulation of Schizochytrium sp. in propionate wastewater

Zhang

et al. 2023

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

In order to find a more effective way to obtain docosahexaenoic acid (DHA) rich lipid from Schizochytrium sp., a widespread propionate wastewater (PW) is used. PW is a common industrial and domestic wastewater, and transforming it into valuable products is a potential treatment method. Schizochytrium sp. is a rapidly growing oleaginous organism, which has been used commercially for DHA production. Herein, PW is completely used for DHA production by Schizochytrium sp. by genetic engineering and fermentation optimization, which can alleviate the increasingly tense demand for water resources and environmental pollution caused by industrial wastewater. Firstly, the methylmalonyl‐CoA mutase (MCM) was overexpressed in Schizochytrium sp. to enhance the metabolism of propionate, then the engineered strain of overexpressed MCM (OMCM) can effectively use propionate. Then, the effects of PW with different concentration of propionate were investigated, and results showed that OMCM can completely replace clean water with PW containing 5 g L−1 propionate. Furthermore, in the fed‐batch fermentation, the OMCM obtained the highest biomass of 113.4 g L−1 and lipid yield of 64.4 g L−1 in PW condition, which is 26.8% and 51.7% higher than that of wild type (WT) in PW condition. Moreover, to verify why overexpression of MCM can promote DHA and lipid accumulation, the comparative metabolomics, ATP production level, the antioxidant system, and the transcription of key genes were investigated. Results showed that ATP induced by PW condition could drive the synthesis of DHA, and remarkably improve the antioxidant capacity of cells by enhancing the carotenoids production. Therefore, PW can be used as an effective and economical substrate and water source for Schizochytrium sp. to accumulate biomass and DHA.

show abstract

“…Its DCW and total lipid can reach 165.0 and 113.6 g/L, respectively, under specific circumstances [ 29 ]. Our previous research had shown that HX-308 has relatively high tolerance to furfural derivatives and organic acids found in lignocellulosic hydrolysates [ 30 ]. However, HX-308 is unable to use xylose to safeguard cell growth, which limits the utilization of lignocellulose.…”

Section: Introductionmentioning

confidence: 99%

Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose

Wang

Zhang

Nong

et al. 2022

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Schizochytrium sp. is a heterotrophic, oil-producing microorganism that can efficiently produce lipids. However, the industrial production of bulk chemicals using Schizochytrium sp. is still not economically viable due to high-cost culture medium. Replacing glucose with cheap and renewable lignocellulose is a highly promising approach to reduce production costs, but Schizochytrium sp. cannot efficiently metabolize xylose, a major pentose in lignocellulosic biomass. Results In order to improve the utilization of lignocellulose by Schizochytrium sp., we cloned and functionally characterized the genes encoding enzymes involved in the xylose metabolism. The results showed that the endogenous xylose reductase and xylulose kinase genes possess corresponding functional activities. Additionally, attempts were made to construct a strain of Schizochytrium sp. that can effectively use xylose by using genetic engineering techniques to introduce exogenous xylitol dehydrogenase/xylose isomerase; however, the introduction of heterologous xylitol dehydrogenase did not produce a xylose-utilizing engineered strain, whereas the introduction of xylose isomerase did. The results showed that the engineered strain 308-XI with an exogenous xylose isomerase could consume 8.2 g/L xylose over 60 h of cultivation. Xylose consumption was further elevated to 11.1 g/L when heterologous xylose isomerase and xylulose kinase were overexpressed simultaneously. Furthermore, cultivation of 308-XI-XK(S) using lignocellulosic hydrolysates, which contained glucose and xylose, yielded a 22.4 g/L of dry cell weight and 5.3 g/L of total lipid titer, respectively, representing 42.7 and 30.4% increases compared to the wild type. Conclusion This study shows that engineering of Schizochytrium sp. to efficiently utilize xylose is conducive to improve its utilization of lignocellulose, which can reduce the costs of industrial lipid production.

show abstract

Assessing the potential of Schizochytrium sp. HX‐308 for microbial lipids production from corn stover hydrolysate

Cited by 10 publications

References 38 publications

Hydrodynamic studies of innovative membrane reactor for enzymatic hydrolysis of lignocellulosic waste

Hydrodynamic studies of innovative membrane reactor for enzymatic hydrolysis of lignocellulosic waste

Enhancing the biomass and docosahexaenoic acid‐rich lipid accumulation of Schizochytrium sp. in propionate wastewater

Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose

Contact Info

Product

Resources

About