Assessing the performance of synthetic co-cultures during the conversion of methane into Poly(3-hydroxybutyrate)

Amabile, Claudia; Abate, Teresa; Chianese, Simeone; Musmarra, Dino; Muñoz, Raul

doi:10.1016/j.cej.2023.146646

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…In Cycle 1, OBAS cultures, particularly OBAS (1:1), exhibited higher optical density compared to OB3b-only cultures, reaching a final optical density of 0.659 ± 0.056. Approximately 31% of CH 4 and 40% of O 2 were consumed within 72 h in OBAS (1:1) cultures, with a CH 4 to O 2 consumption ratio of 1.29, slightly lower than that observed in previous studies [19,[38][39][40]. AS-only and OBAS (1:4) cultures displayed lower optical densities of 0.371 ± 0.150, and 0.176 ± 0.031, respectively.…”

Section: Growth Characteristics Of M Trichosporium Ob3b-amended Culturescontrasting

confidence: 61%

Methylosinus trichosporium OB3b bioaugmentation unleashes polyhydroxybutyrate-accumulating potential in waste-activated sludge

Eam,

Ko,

Lee

et al. 2024

Microb Cell Fact

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Background Wastewater treatment plants contribute approximately 6% of anthropogenic methane emissions. Methanotrophs, capable of converting methane into polyhydroxybutyrate (PHB), offer a promising solution for utilizing methane as a carbon source, using activated sludge as a seed culture for PHB production. However, maintaining and enriching PHB-accumulating methanotrophic communities poses challenges. Results This study investigated the potential of Methylosinus trichosporium OB3b to bioaugment PHB-accumulating methanotrophic consortium within activated sludge to enhance PHB production. Waste-activated sludges with varying ratios of M. trichosporium OB3b (1:0, 1:1, 1:4, and 0:1) were cultivated. The results revealed substantial growth and methane consumption in waste-activated sludge with M. trichosporium OB3b-amended cultures, particularly in a 1:1 ratio. Enhanced PHB accumulation, reaching 37.1% in the same ratio culture, indicates the dominance of Type II methanotrophs. Quantification of methanotrophs by digital polymerase chain reaction showed gradual increases in Type II methanotrophs, correlating with increased PHB production. However, while initial bioaugmentation of M. trichosporium OB3b was observed, its presence decreased in subsequent cycles, indicating the dominance of other Type II methanotrophs. Microbial community analysis highlighted the successful enrichment of Type II methanotrophs-dominated cultures due to the addition of M. trichosporium OB3b, outcompeting Type I methanotrophs. Methylocystis and Methylophilus spp. were the most abundant in M. trichosporium OB3b-amended cultures. Conclusions Bioaugmentation strategies, leveraging M. trichosporium OB3b could significantly enhance PHB production and foster the enrichment of PHB-accumulating methanotrophs in activated sludge. These findings contribute to integrating PHB production in wastewater treatment plants, providing a sustainable solution for resource recovery. Graphical abstract

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Section: Growth Characteristics Of M Trichosporium Ob3b-amended Culturescontrasting

confidence: 61%

Methylosinus trichosporium OB3b bioaugmentation unleashes polyhydroxybutyrate-accumulating potential in waste-activated sludge

Eam,

Ko,

Lee

et al. 2024

Microb Cell Fact

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Section: Introductionmentioning

confidence: 99%

“…Therefore, M. parvus was selected as the main PHA producer, and Rhodococcus opacus was added as partner microbe for cocultivation since the combination of these two strains resulted in a synergistic effect among the species. 40 Valeric acid was fed as a cosubstrate for triggering 3-HV formation. Then, the synthesis of 3-HV monomers, both in the presence and absence of methane, was studied by replacing pure valeric acid with a mixture of four volatile fatty acids (acetic, butyric, valeric, and propionic acids) that simulated the mixture extracted from a real effluent of the hydrolytic fermentation of food waste at 35 °C.…”

Section: Introductionmentioning

confidence: 99%

Exploring New Strategies for Optimizing the Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Methane and VFAs in Synthetic Cocultures and Mixed Methanotrophic Consortia

Amabile,

Abate,

Marcos

et al. 2024

ACS Sustainable Chem. Eng.

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In this work, the potential of a synthetic coculture and a mixed methanotrophic consortium to synthesize poly(3hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from renewable and waste-based feedstocks was assessed batchwise. Methylocystis parvuscocultivated with Rhodococcus opacus and a Methylocystisenriched culture previously grown on methane were subjected to nutrient starvation in a medium enriched with valeric acid (30% w w −1 of C tot ) or with a VFAs mixture containing acetic, propionic, butyric, and valeric acids (15% w w −1 of C tot ) under a CH 4 :O 2 or air atmosphere. For all test series, pH was adjusted to 7 after adding the cosubstrates, and a negligible substrate consumption or polymer production was considered the end point of the trial. Results showed that valeric acid promoted PHBV accumulation in both cultures regardless of the atmosphere. Interestingly, the mixture of VFAs supported PHBV accumulation only in the presence of methane. The highest PHBV contents for the coculture and the mixed consortium, equal to 73.7 ± 2.5% w w −1 and 49.6 ± 13% w w −1 , respectively, were obtained with methane and the VFAs mixture. This study demonstrates the suitability of cocultures and biobased cosubstrates for the sustainable production of the biodegradable polymer PHBV.

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A newly isolated strain for poly(3-hydroxybutyrate) production under anaerobic conditions and the key enzyme analysis

Ma,

Li,

Tyagi

et al. 2024

Chemical Engineering Journal

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Assessing the performance of synthetic co-cultures during the conversion of methane into Poly(3-hydroxybutyrate)

Cited by 9 publications

References 36 publications

Methylosinus trichosporium OB3b bioaugmentation unleashes polyhydroxybutyrate-accumulating potential in waste-activated sludge

Methylosinus trichosporium OB3b bioaugmentation unleashes polyhydroxybutyrate-accumulating potential in waste-activated sludge

Exploring New Strategies for Optimizing the Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Methane and VFAs in Synthetic Cocultures and Mixed Methanotrophic Consortia

A newly isolated strain for poly(3-hydroxybutyrate) production under anaerobic conditions and the key enzyme analysis

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