Assessing the occurrence of a stress syndrome in mussels (Mytilus edulis) using a combined biomarker/gene expression approach

Dondero, Francesco; Dagnino, A.; Jönsson, Henrik; Caprì, Flavia; Gastaldi, Laura; Viarengo, Aldo

doi:10.1016/j.aquatox.2006.02.025

Cited by 96 publications

(46 citation statements)

References 44 publications

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“…For example mussels are important for biomonitoring (24) and have been widely used in studies of population genetics and speciation (25,26). Given the increasing interest in the use of proteomics techniques in marine biology (27,28), mussels are thus positioned to play an important future role in this area.…”

mentioning

confidence: 99%

Genetic Variation Underlying Protein Expression in Eggs of the Marine Mussel Mytilus edulis

Diz

Dudley

MacDonald

et al. 2009

Molecular & Cellular Proteomics

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Study of the genetic basis of gene expression variation is central to attempts to understand the causes of evolutionary change. Although there are many transcriptomics studies estimating genetic variance and heritability in model organisms such as humans there is a lack of equivalent proteomics studies. In the present study, the heritability underlying egg protein expression was estimated in the marine mussel Mytilus. We believe this to be the first such measurement of genetic variation for gene expression in eggs of any organism. The study of eggs is important in evolutionary theory and life history analysis because maternal effects might have profound effects on the rate of evolution of offspring traits. Evidence is presented that the egg proteome varies significantly between individual females and that heritability of protein expression in mussel eggs is moderate to high suggesting abundant genetic variation on which natural selection might act. The study of the mussel egg proteome is also important because of the unusual system of mitochondrial DNA inheritance in mussels whereby different mitochondrial genomes are transmitted independently through female and male lineages (doubly uniparental inheritance). It is likely that the mechanism underlying this system involves the interaction of specific egg factors with sperm mitochondria following fertilization, and its elucidation might be advanced by study of the proteome in females having different progeny sex ratios. Putative identifications are presented here for egg proteins using MS/MS in Mytilus lines differing in sex ratio. Ontology terms relating to stress response and protein folding occur more frequently for proteins showing large expression differences between the lines. The distribution of ontology terms in mussel eggs was compared with those for previous mussel proteomics studies (using other tissues) and with mammal eggs. Significant differences were observed between mussel eggs and mussel tissues but not between the two types of eggs. Molecular & Cellular Proteomics 8:132-144, 2009.

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confidence: 99%

Genetic Variation Underlying Protein Expression in Eggs of the Marine Mussel Mytilus edulis

Diz

Dudley

MacDonald

et al. 2009

Molecular & Cellular Proteomics

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“…Bivalve molluscs are commonly used as bio-monitors of marine and estuarine pollution because, as filter-feeding organisms, they are efficient accumulators of organic and metal contaminants and display great sensitivity to pollutants (Romeo et al 2003;Dondero et al 2006;Lobo et al 2010). These animals have a high ecological value due to their sedentary life and filtration capacity and they provide numerous ecosystem services in estuaries (Coen et al 2007;Maria et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Indicators of environmental stress: cellular biomarkers and reproductive responses in the Sydney rock oyster (Saccostrea glomerata)

Edge

Johnston

Roach³

et al. 2012

Ecotoxicology

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We measured a suite of common biomarker responses for the first time in the Sydney rock oyster Saccostrea glomerata to evaluate their utility as biological effects measures for pollution monitoring. To examine the relationship between biomarker responses and population level effects, fertilisation and embryo development assays were also conducted. Adult oysters were deployed in two contaminated estuaries and a reference estuary in Sydney, Australia. The concentrations of various contaminants (metals and polycyclic aromatic hydrocarbon, PAHs) were quantified in oyster's tissue from each site and both metals and total PAHs were significantly elevated in contaminated estuaries relative to the reference estuary. Lysosomal membrane destabilisation, lipid peroxidation levels and glutathione (GSH) concentrations were measured in the digestive gland of oysters. Of all biomarkers measured, lysosomal membrane destabilisation proved to be the most useful indicator of oysters facing anthropogenic stress and we suggest this may be an especially useful biomarker for incorporation into local environmental monitoring programs. Moreover, lysosomal membrane destabilisation showed good correlations with fertilisation, normal embryo development and estuary status. GSH and lipid peroxidation were not as valuable for distinguishing between estuaries exposed to differing levels of anthropogenic stress, but did provide additional valuable information regarding overall health status of the oysters.

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“…Recent developments in biology have greatly expanded the single biomarker approach to the global analysis of molecules in a biological system, which has led to the development of Omics techniques. including genomics, proteomics, transcriptomics, and metabolomics [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Tissue‐specific toxicological effects of cadmium in green mussels (Perna viridis): Nuclear magnetic resonance‐based metabolomics study

Wang

2011

Enviro Toxic and Chemistry

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Toxicity tests for metals have traditionally focused on selected biomarkers to characterize the biological stress induced by metals in marine organisms. Here nuclear magnetic resonance (NMR)-based metabolomics, a system biology tool, was applied to the marine green mussel, Perna viridis, to investigate the toxicological effects of Cd in both digestive gland and adductor muscle tissues. After Cd exposure for either two or four weeks, there was no significant metabolic change in the mussels exposed to Cd at 2 µg/L. At 20 µg/L, there were major metabolite changes related to amino acids, osmolytes, and energy metabolites. Digestive gland tissue was more sensitive to Cd than adductor muscle tissue. The adductor muscle tissue showed elevated levels of glutamine, glutamate, and lactate, and reduced levels of branched chain amino acids, aspartate, phenylalanine, and tyrosine. Overall, four weeks of Cd exposure produced neurotoxicity and metabolic disturbances and disturbed osmoregulation. These results suggest that the adductor muscle tissue of mussels may be a suitable supplemental biomarker for exposure to toxicants. In addition, the results demonstrate that (1) H-NMR-based metabolomic analysis can provide a systematic view of the toxicological effects of metals on mussels, suggesting that it might be employed to investigate the toxicological effects of other marine pollutants.

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Assessing the occurrence of a stress syndrome in mussels (Mytilus edulis) using a combined biomarker/gene expression approach

Cited by 96 publications

References 44 publications

Genetic Variation Underlying Protein Expression in Eggs of the Marine Mussel Mytilus edulis

Genetic Variation Underlying Protein Expression in Eggs of the Marine Mussel Mytilus edulis

Indicators of environmental stress: cellular biomarkers and reproductive responses in the Sydney rock oyster (Saccostrea glomerata)

Tissue‐specific toxicological effects of cadmium in green mussels (Perna viridis): Nuclear magnetic resonance‐based metabolomics study

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