Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine

Carton, Flavia; Malatesta, Manuela

doi:10.4081/ejh.2022.3603

Cited by 2 publications

(1 citation statement)

References 73 publications

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“…Hydrogels have also been used for the 3D reconstruction in vitro of human skin for experimental purposes. [8][9][10] Considering the increasing interest on hydrogels designed for skin repair and regeneration, this paper aims to present an overview of the most recent progresses in the development of high-performance hydrogels, highlighting the great contribution of immunohistochemistry (IHC) to the evaluation of the therapeutic potential of these innovative medical devices.…”

Section: Introductionmentioning

confidence: 99%

The contribution of immunohistochemistry to the development of hydrogels for skin repair and regeneration

Carton

2023

Eur J Histochem

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Hydrogels based on various polymeric materials have been successfully developed in recent years for a variety of skin applications. Several studies have shown that hydrogels with regenerative, antibacterial, and antiinflammatory properties can provide faster and better healing outcomes, particularly in chronic diseases where the normal physiological healing process is significantly hampered. Various experimental tests are typically performed to assess these materials' ability to promote angiogenesis, re-epithelialization, and the production and maturation of new extracellular matrix. Immunohistochemistry is important in this context because it allows for the visualization of in situ target tissue factors involved in the various stages of wound healing using antibodies labelled with specific markers detectable with different microscopy techniques. This review provides an overview of the various immunohistochemical techniques that have been used in recent years to investigate the efficacy of various types of hydrogels in assisting skin healing processes. The large number of scientific articles published demonstrates immunohistochemistry's significant contribution to the development of engineered biomaterials suitable for treating skin injuries.

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Section: Introductionmentioning

confidence: 99%

The contribution of immunohistochemistry to the development of hydrogels for skin repair and regeneration

Carton

2023

Eur J Histochem

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Chitosan-Tricarbocyanine-Based Nanogels Were Able to Cross the Blood–Brain Barrier Showing Its Potential as a Targeted Site Delivery Agent

Rivera López,

Samaniego López,

Spagnuolo

et al. 2024

Pharmaceutics

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Targeting drugs to the central nervous system (CNS) is challenging due to the presence of the blood–brain barrier (BBB). The cutting edge in nanotechnology generates optimism to overcome the growing challenges in biomedical sciences through the effective engineering of nanogels. The primary objective of the present report was to develop and characterize a biocompatible natural chitosan (CS)-based NG that can be tracked thanks to the tricarbocyanine (CNN) fluorescent probe addition on the biopolymer backbone. FTIR shed light on the chemical groups involved in the CS and CNN interactions and between CNN-CS and tripolyphosphate, the cross-linking agent. Both in vitro and in vivo experiments were carried out to determine if CS-NGs can be utilized as therapeutic delivery vehicles directed towards the brain. An ionic gelation method was chosen to generate cationic CNN-CS-NG. DLS and TEM confirmed that these entities’ sizes fell into the nanoscale. CNN-CS-NG was found to be non-cytotoxic, as determined in the SH-SY5Y neuroblastoma cell line through biocompatibility assays. After cellular internalization, the occurrence of an endo-lysosomal escape (a crucial event for an efficient drug delivery) of CNN-CS-NG was detected. Furthermore, CNN-CS-NG administered intraperitoneally to female CF-1 mice were detected in different brain regions after 2 h of administration, using fluorescence microscopy. To conclude, the obtained findings in the present report can be useful in the field of neuro-nanomedicine when designing drug vehicles with the purpose of delivering drugs to the CNS.

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Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine

Cited by 2 publications

References 73 publications

The contribution of immunohistochemistry to the development of hydrogels for skin repair and regeneration

The contribution of immunohistochemistry to the development of hydrogels for skin repair and regeneration

Chitosan-Tricarbocyanine-Based Nanogels Were Able to Cross the Blood–Brain Barrier Showing Its Potential as a Targeted Site Delivery Agent

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