Assessing the In Vitro Effectiveness of Antimicrobials against
            <i>Mycoplasma mycoides</i>
            subsp.
            <i>mycoides</i>
            Small-Colony Type To Reduce Contagious Bovine Pleuropneumonia Infection

Ayling, Roger D.; Bisgaard-Frantzen, S.; March, John B.; Godinho, Kevin; Nicholas, R. A. J.

doi:10.1128/aac.49.12.5162-5165.2005

Cited by 24 publications

(18 citation statements)

References 5 publications

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“…Moreover, their MIC 50 and MIC 90 values showed that these antimicrobials are not effective against this pathogen. These results are in agreement with those described in previous studies performed on other mycoplasma species [8, 10, 11, 33]. Hence, our work supports the previously proposed hypothesis about an intrinsic resistance of the mycoplasmas of the M .…”

Section: Discussionsupporting

confidence: 94%

Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Tatay-Dualde

Ham

et al. 2017

PLoS ONE

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Mycoplasma capricolum subsp. capricolum is one of the causative agents of contagious agalactia (CA). Nevertheless, there is still a lack of information about its antimicrobial susceptibility and genetic characteristics. Therefore, the aim of this work was to study the antimicrobial and genetic variability of different Mycoplasma capricolum subsp. capricolum field isolates. For this purpose, the growth inhibition effect of 18 antimicrobials and a multilocus sequence typing (MLST) scheme based on five housekeeping genes (fusA, glpQ, gyrB, lepA and rpoB) were performed on 32 selected field isolates from Italy and Spain.The results showed a wide range of growth inhibitory effects for almost all the antimicrobials studied. Macrolides presented lower efficacy inhibiting Mcc growth than in previous works performed on other CA-causative mycoplasmas. Erythromycin was not able to inhibit the growth of any of the studied strains, contrary to doxycycline, which inhibited the growth of all of them from low concentrations. On the other hand, the study of the concatenated genes revealed a high genetic variability among the different Mcc isolates. Hence, these genetic variations were greater than the ones reported in prior works on other mycoplasma species.

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Section: Discussionsupporting

confidence: 94%

Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Tatay-Dualde

Ham

et al. 2017

PLoS ONE

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“…A number of products have demonstrated efficacy against MmmSC in vitro, including oxytetracycline (OTC), danofloxacin and tilmicosin (Ayling et al, 2000(Ayling et al, , 2005. Modelling studies have predicted that antimicrobials, used in association with vaccination, could be successful in eradicating disease from infected herds (Mariner et al, 2006b).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antimicrobial activity against Mycoplasma mycoides subsp. mycoides Small Colony using an in vitro dynamic dilution pharmacokinetic/pharmacodynamic model

Mitchell

McKellar

McKeever

2013

Journal of Medical Microbiology

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The objectives of this study were to assess the activity of oxytetracycline (OTC), danofloxacin and tulathromycin against Mycoplasma mycoides subsp. mycoides Small Colony, the causative agent of contagious bovine pleuropneumonia, in an in vitro dynamic concentration model and to determine the concentration and/or time dependence of such activity. Time-kill assays that simulated elimination of antimicrobials from the body were performed. Initial antimicrobial concentrations corresponded to various multiples of the MIC and cultures were diluted in a stepwise fashion with either drug-free or drug-containing artificial medium to mimic administration by single-release bolus or infusion, respectively. Where appropriate, data were fitted to sigmoidal E max models. OTC produced no change in mycoplasma titre from the initial inoculum size, regardless of the concentration or means of drug exposure. Both danofloxacin and tulathromycin resulted in a decrease in mycoplasma titre but neither was bactericidal (99.9 % kill) over 12 h. A greater antimycoplasmal effect, defined as the change in log 10 (c.f.u. ml ) over 12 h, was achieved when danofloxacin was administered as a single-release bolus, suggesting concentration-dependent activity, whereas the antimycoplasmal effect of tulathromycin was comparable following administration by single-release bolus or infusion, owing to its long half-life.

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“…The increasing use of antibiotics by herdsmen despite the risk of creating a carrier state in an animal is probably an attempt to alleviate the suffering of the affected cattle as reported by Mariner et al (2006). However, , Yaya et al (2004) and Ayling et al (2005) stated some beneficial effects on the use of antibiotics in the treatment of CBPP amongst which include limiting the clinical severity of the disease. But however, complete elimination of the causative organism from the affected cattle may not be achieved, thereby creating a potential pool of disease carrier.…”

Section: Resultsmentioning

confidence: 99%

Awareness, knowledge and practices of pastoralists towards contagious bovine pleuro pneumonia in Kaduna State, Nigeria

I¹,

A²,

A³

et al. 2015

J. Vet. Med. Anim. Health

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This study was conducted to determine the awareness, knowledge and practices of pastoralists on contagious bovine pleuropneumonia (CBPP) in cattle in 3 selected Local Government Areas (LGAs) (Ikara, Chikun and Kauru) of Kaduna State. A cattle population of a minimum of 20 heads was considered as a herd in this study and a total of 90 open and close ended structured questionnaires were designed and administered to all the 90 herdsmen that gave their consent for this study. The study revealed that, the level of awareness of CBPP by the pastoralists was 80 (88.9%) and formal education of 11 (12.2%), while knowledge of CBPP in the communities showed 66 (73.3%). Those that experienced outbreaks, as well as lost animals to CBPP, were 25 (27.8%) and 17 (18.9%), respectively. Of the respondents, 67 (74.4%) admitted vaccinating their cattle at various times, which is not regular, thereby achieving a low vaccination coverage of 36.7%, while, 23 (25.5%) do not vaccinate at all. Fourty-six (51.1%) acquired the CBPP vaccines from the National Veterinary Research Institute (NVRI), Vom, Plateau State, while, 21 (23.3%) from the open market. Personnel used in the administration of vaccine were; Veterinarians 14 (15.6%), animal health workers 18(20%), drug vendors 18 (20%) and herdsmen 40 (44.4%). The study has shown that most pastoralists within the study area are aware of CBPP and have good knowledge of the disease. However, unethical practices such as sourcing and indiscriminate administering of vaccines as well as irregular vaccination schedule may hamper successful disease control. Pastoralists should be encouraged to intensify the pursuit for formal education.

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Assessing the In Vitro Effectiveness of Antimicrobials against Mycoplasma mycoides subsp. mycoides Small-Colony Type To Reduce Contagious Bovine Pleuropneumonia Infection

Cited by 24 publications

References 5 publications

Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Evaluation of antimicrobial activity against Mycoplasma mycoides subsp. mycoides Small Colony using an in vitro dynamic dilution pharmacokinetic/pharmacodynamic model

Awareness, knowledge and practices of pastoralists towards contagious bovine pleuro pneumonia in Kaduna State, Nigeria

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