Assessing the impact of corn steep liquor as an inducer on enhancing laccase production and laccase gene (Lac1) transcription in Pleurotus pulmonarius during solid-state fermentation

Suresh, Geethu; Ragunathan, R.; Johney, Jesteena

doi:10.1016/j.biteb.2024.101905

Bioresource Technology Reports

2024

DOI: 10.1016/j.biteb.2024.101905

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Assessing the impact of corn steep liquor as an inducer on enhancing laccase production and laccase gene (Lac1) transcription in Pleurotus pulmonarius during solid-state fermentation

Geethu Suresh,

R. Ragunathan,

Jesteena Johney

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2024

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Esterification Process in Supercritical Carbon Dioxide Catalyzed by Geotrichum candidum Lipase Produced with Mozzarella Cheese Whey and Corn Steep Liquor

Ramos,

de Oliveira,

Ramos

et al. 2024

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The valorization of industrial waste in the production of new products is a growing trend, with food waste showing significant promise as a raw material for various industries. Thus, this research aimed to investigate the production of Geotrichum candidum lipase using industrial waste, such as mozzarella cheese whey (MCW) and corn steep liquor (CSL), and to analyze how effectively it catalyzes the esterification of oleic acid with methanol. Lipase production was carried out in medium containing MCW and CSL, with fermentation conditions optimized using a fractional factorial experimental design and central composite experimental design. The highest activities (16.71 U/mL in 24 h and 17.80 U/mL in 48 h) were found in conditions of 13.6% (%m/v) CSL and 37.1% (%m/v) MCW, with corn oil fixed at 1% (%m/v) and pH fixed at 6,00. Esterification conditions were evaluated at atmospheric pressure, both in a solvent-free environment and using hexane, as well as under high pressures with supercritical carbon dioxide (SC-CO2). The produced lipase demonstrated high catalytic activity for the esterification reaction of oleic acid with methanol under SC-CO2 with an increase of 39.41% in the yield. The yields found confirm the feasibility of producing lipase from industrial waste, thus presenting it as a sustainable and efficient alternative for industrial processes, and show that there is no need to use toxic organic solvents in esterification reactions.

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Esterification Process in Supercritical Carbon Dioxide Catalyzed by Geotrichum candidum Lipase Produced with Mozzarella Cheese Whey and Corn Steep Liquor

Ramos,

de Oliveira,

Ramos

et al. 2024

Processes

View full text Add to dashboard Cite

show abstract

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Assessing the impact of corn steep liquor as an inducer on enhancing laccase production and laccase gene (Lac1) transcription in Pleurotus pulmonarius during solid-state fermentation

Cited by 1 publication

References 36 publications

Esterification Process in Supercritical Carbon Dioxide Catalyzed by Geotrichum candidum Lipase Produced with Mozzarella Cheese Whey and Corn Steep Liquor

Esterification Process in Supercritical Carbon Dioxide Catalyzed by Geotrichum candidum Lipase Produced with Mozzarella Cheese Whey and Corn Steep Liquor

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