Assessing the genotoxic potentials of arsenic in tilapia (Oreochromis mossambicus) using alkaline comet assay and micronucleus test

Ahmed, Md. Kawser; Habibullah-Al-Mamun, Md.; Hossain, Mohammed Anwar; Arif, Murtaza; Parvin, Elora; Akter, Mosammat Salma; Khan, Mohammad Rezwan; Islam, Monirul

doi:10.1016/j.chemosphere.2011.02.025

Cited by 93 publications

(51 citation statements)

References 50 publications

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“…These findings are similar to the Ahmed et al (2011) who also observed concentration dependent increase in micronuclei frequency in the erythrocytes of Oreochromis mossambicus due to arsenic. Moreover, in Channa punctatus, the frequency of micronuclei was found to be significantly higher due to arsenic exposure while it was lower in control fish (Patowary et al, 2012).…”

Section: Discussionsupporting

confidence: 80%

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Kousar¹,

Javed²

2015

Turk. J. Fish. Aquat. Sci.

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A wide variety of chemicals especially metals contaminate the aquatic ecosystem and stimulate a variety of toxicity mechanisms, such as oxidative damage to proteins, DNA and lipids. Very limited efforts have been made to investigate the genotoxic effects of metals in fish. Therefore, the present study was conducted to determine copper induced genotoxic damage in peripheral blood erythrocytes of 150-day old four freshwater fish species viz. Labeo rohita, Cirrhina mrigala, Catla catla and Ctenopharyngodon idella by using micronucleus test. For this purpose, 96-hr LC50 and lethal concentration of copper for all the four fish species were determined. Each fish species was exposed to four different sub-lethal concentrations (17, 25, 33, 50% of 96-hr LC50) of copper, separately, for a period of 30 days. Results showed exposure concentration dependent increases in the frequencies of micronuclei and other nuclear abnormalities. Findings of the experiment also confirmed that the micronucleus test acts as a useful tool in determining the potential genotoxicity of metals in erythrocytes of various fish species. Among four fish species, Cirrhina mrigala showed significantly higher frequency of micronuclei with the mean value of 16.98±11.70%, followed by that of Ctenopharyngodon idella, Catla catla and Labeo rohita. However, frequency of other nuclear abnormalities followed the order: Labeo rohita > Cirrhina mrigala > Ctenopharyngodon idella > Catla catla.

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Section: Discussionsupporting

confidence: 80%

Untitled

Kousar¹,

Javed²

2015

Turk. J. Fish. Aquat. Sci.

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“…There was only single study evaluating DNA damage in ovarian tissue of rat [29] and in liver of fish [39] exposed to different doses of arsenic and indicated the increased extent of DNA damage with all doses, specifically at high dose levels. In studies of current review, arsenic concentration was measured in blood, liver, ovary and water.…”

Section: Discussionmentioning

confidence: 99%

“…Arsenic induced genotoxicity has also been calculated in animals exposed to different doses of arsenic and significant DNA damage has been observed in mice [38] rats [29], and fish [39].…”

Section: Detail Description Of Findingsmentioning

confidence: 99%

Assessment of Arsenic Induced DNA Fragmentation by using Comet Assay

Mahjabeen¹

2016

J Carcinog Mutagen

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Arsenic is metalloid present in measureable quantity in air, water, and soil through natural and anthropogenic sources. It is neurotoxic, hepatotoxic and genotoxic effects, variety of health problems have been associated to arsenic exposure. This review was designed to investigate the possible association between arsenic exposure and DNA damage in animals and humans using comet assay. Total 28 studies were selected for measuring DNA damage by comet assay. Trend of significance in tail length and tail moment was observed using regression analysis. Due to limited number of studies available the regression analysis was non-significant. Individually each study suggested a significant increase in tail moment and tail length in a dose and time-dependent manner. Overall trend observed in this review is the positive association between arsenic exposure either experimentally or occupationally and DNA damage. This initial effort may provide future guideline for the assessment of DNA fragmentation using comet assay.

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“…The data showed that toxicity of methomyl-based exposure against Tilapia increased simultaneously with increased of doses and time exposure. A chemical toxicity increased against organism simultaneously with increasing of dose and time of exposure [24]. Base on the result of probit analysis that show in Figure 3, can be find out of LC50 96 hours methomyl-based against Tilapia (Oreochromis niloticus).…”

Section: Definitive Testmentioning

confidence: 99%

Assessing the Genotoxic Potentials of Methomyl-based Pesticide in Tilapia (Oreochromis niloticus) Using Micronucleus Assay

Islamy

Yanuhar

Hertika

2017

JELS

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Pesticides are recognized as serious pollutants in the aquatic environment with the potential to cause genotoxic on the aquatic organism, especially fish. The micronucleus (MN) assay has been used to evaluate genotoxicity of many compounds in polluted ecosystems such pesticides. im of this study to determine genotoxic effect of methomylbased pesticide on tilapia (Oreochromis niloticus). Fish were exposed to six different concentrations base on range finding test (0 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, 8.7 ppm and 10 ppm) of methomyl-based pesticide. The micronucleus were collected from peripheral blood erythrocyte of fish after 96 h exposure. Peripheral blood samples smears were stained with Giemsa, MN frequencies were counted and statistically analyzed using one-way ANOVA. The result of this study showed after 96 hours exposed to methomyl-based pesticide, at concentration 0 ppm causes 0% mortality, at concentration to 3.2 ppm causes 30% mortality, at concentration 4.2 ppm causes 60% mortality, at concentration 6.5 ppm causes 70% mortality, at concentration 8.7 ppm causes 80% mortality, at concentration 10 ppm causes 100% mortality of fish test. Lethal Concentration 50 (LC50 -96 hours) of methomy-base pesticide towards tilapia (O. niloticus) is 4.015 ppm. Through micronuclei assay during 96 hour exposure of methomyl-based pesticide, the result shows that frequencies of micronuclei in erythrocyte of fish test at concentration at 0ppm is 12‰, 18‰ and 16‰; at concentration at 3.2ppm is 33‰, 26‰ and 29‰; at concentration at 4.2ppm is 41‰, 38‰ and 46‰; at concentration at 6.5ppm is 68‰, 81‰ and 82‰; at concentration 8.7ppm is 133‰, 130‰ and 137‰; at concentration 10ppm is 163‰, 166‰ and 156‰. It revealed that methomyl-based pesticide exposure induced after 96 h significantly (P<0.05) increased genotoxic potentials simultaneous with increased concentration.

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Assessing the genotoxic potentials of arsenic in tilapia (Oreochromis mossambicus) using alkaline comet assay and micronucleus test

Cited by 93 publications

References 50 publications

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Assessment of Arsenic Induced DNA Fragmentation by using Comet Assay

Assessing the Genotoxic Potentials of Methomyl-based Pesticide in Tilapia (Oreochromis niloticus) Using Micronucleus Assay

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