Assessing the Genetic Stability of In Vitro Raised Plants

Rohela, Gulab Khan; Jogam, Phanikanth; Saini, Pawan; Sandhya, Dulam; Peddaboina, Venkataiah; Shekhawat, Mahipal S.

doi:10.1007/978-981-19-0055-6_11

Cited by 7 publications

(2 citation statements)

References 196 publications

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“…During the period of culturing, variable responses of plants to an in vitro environment may be accompanied by genetic variations (Ai et al, 2023). Generally, multiplication techniques via buds (existing meristems), shoot tips, nodes, internodes, leaves, and petioles are most frequently chosen to maintain genetic stability (Sliwinska, Thiem, 2007;Rohela et al, 2022). However, newly developed microplants grown from the meristems of the in vitro propagated shoots may show genetic variability (Sliwinska, Thiem, 2007).…”

Section: Introductionmentioning

confidence: 99%

Anatomy, ploidy level, and essential oil composition of Hyssopus officinalis ʻNikitskiy Beliyʼ in vitro and ex situ

Bulavin,

Ivanova,

Miroshnichenko

et al. 2024

Trudy po prikladnoy botanike, genetike i selektsii

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Background. Clonal micropropagation is a biotechnological method for plant multiplication. The existing data on the structure of organs in vitro, genetic stability, and essential oil composition are limited for Hyssopus officinalis L., so this study was aimead at investigating these aspects under a short period of in vitro culturing.Materials and methods. Plants of Hyssopus officinalis ʻNikitskiy Beliyʼ cultivated ex situ, in vitro and ex vitro were analyzed. Conventional methods were applied to study plant anatomy, ploidy level, and relative DNA content, as well as to extract and analyze essential oil. Statistical analysis was performed using the Past 4.03 software.Results. According to the results obtained, with 6-BAP introduced into MS nutrient medium in optimal concentrations (0.3– 0.5 mg/L), the general in vitro structure of leaf blades in the developed microshoots was similar to those in ex situ plants, while the qualitative and quantitative changes observed were induced by the effect of specific culturing conditions and plant rejuvenation. The analysis of the ploidy level and relative DNA content in the nuclei isolated from the leaf tissue cells of the microshoots ex vitro after adaptation revealed no changes compared to the ex situ leaf parameters. The mass fraction of essential oil and its component composition in the mother plants and ex vitro regenerants were similar.Conclusion. Cultivation of Hyssopus officinalis ʻNikitskiy Beliyʼ microshoots on MS nutrient medium with 6-BAP optimal concentrations promotes morphogenesis without significant deviations in the ploidy level, relative DNA content, essential oil yield, or its component composition. The developed protocol for clonal micropropagation of Hyssopus officinalis ʻNikitskiy Beliyʼ provides clones identical to the ex situ plants.

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Section: Introductionmentioning

confidence: 99%

Anatomy, ploidy level, and essential oil composition of Hyssopus officinalis ʻNikitskiy Beliyʼ in vitro and ex situ

Bulavin,

Ivanova,

Miroshnichenko

et al. 2024

Trudy po prikladnoy botanike, genetike i selektsii

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“…Various other crop improvement efforts, such as somaclonal variation and micropropagation, rely on tissue culture techniques. In vitro regeneration after a long cycle of callogenesis may result in genetic variation among undifferentiated cells, protoplasts, or regenerated shoots. , Such somaclonal variants offer a relatively quick way of producing novel genetic variations without the need for genetic modification using sophisticated biotechnological techniques. Somaclonal variations are important for crops that are difficult to breed or have narrow genetic variability .…”

Section: Introductionmentioning

confidence: 99%

In Vitro Plant Regeneration from Petioles of Spinach (Spinacia oleracea L.)

Hussain,

Ali,

Mustafa

et al. 2023

ACS Agric. Sci. Technol.

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Spinach (Spinacia oleracea L.) is an economically important leafy vegetable crop with an annual global production of 32 million tons. However, it is vulnerable to chewing as well as sucking insects. In vitro plant regeneration can significantly contribute to efforts toward crop improvement. This study is aimed at developing a highly efficient protocol for direct in vitro regeneration of S. oleracea from five different explants (cotyledons, leaves, petioles, hypocotyls, and root segments) and five different regeneration media. Results intimated that only petioles exhibited a response for direct regeneration; hence, they were used in further experiments. More than 35% of the petioles regenerated directly into shoots, which were later separated and cultured onto a rooting medium. All other explants showed variable responses; however, none of them could regenerate in vitro. As far as the role of plant growth regulators is concerned, gibberellic acid (GA 3 ) appeared to be the most imperative one for direct in vitro regeneration and bringing about maximum regeneration in Murashige and Skoog (MS) medium augmented with 2 mg L −1 gibberellic acid (GA 3 ), 0.4 mg L −1 naphthalene acetic acid (NAA), and 1 mg L −1 benzyl amino purine (BAP). The resultant plants were acclimatized in the greenhouse and resulted in healthy, fleshy leaves. For the first time, the protocol describes efficient direct in vitro regeneration from green tissues of S. oleracea. As this is an efficient in vitro regeneration system, it may be a step forward to engineer/edit the genome of this green vegetable for valuable traits including agronomic traits improvement, biofortification, and biopharmaceutical production in the future.

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