Assessing the genetic fidelity of somatic embryo-derived plantlets of finger millet by random amplified polymorphic DNA analysis

Venkatesan, Jayalakshmi; Ramu, Vasuki; Sethuraman, Thilaga; Chandrasekaran, S.; Ganesh, D.

doi:10.1007/s10529-022-03305-3

Cited by 2 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CH (500 mg/l) along with KN and IAA induced significantly low number of somatic embryos (15.67 ± 4.2) in more number of days (84.0 ± 1.1). However, casein hydrolysate efficiently induced somatic embryos in Eleusine coracana (L.) Gaertn., Abelmoschus esculentus L. monech and Setaria italica L. (Venkatesan et al, 2022;Daniel et al, 2018;Sood and Prasad, 2020). The somatic embryos were confirmed by observational and histological studies carried out through Olympus BX 51 (Fig.…”

Section: )mentioning

confidence: 71%

Organogenesis and Somatic Embryogenesis for Rapid Multiplication and Regeneration of Polygonatum Verticillatum (L.) : An Ashtavarga Medicinal Herb

Arya,

Divya,

Chaturvedi

2024

View full text Add to dashboard Cite

Polygonatum is a member of Ashtavarga group of medicinal plants that holds high medicinal value in Ayurveda. P. veticillatum (L.) All. is an immunity booster herb. Rhizomes being the medicinally important part of the plant, are harvested from the wild by uprooting the plant, pushing the species at higher threatening risks. Regeneration of this plant through seeds is a slow process as the seeds exhibit double dormancy. Therefore, the study is carried out for the rapid and mass propagation of this medicinal plant through in vitro methods. Leaves and rhizome buds were used as explant for culture establishment. Sterilised explants were inoculated in MS media complimented with various PGRs (mT, IAA, TDZ, NAA, IAA and GA 3 ) and incubated at 25± 2°C with 16/8 hr photoperiod under white LED. Maximum callus (percentage) induction was found in MS medium complemented with TDZ (0.5 mg L -1 ) and 2, 4 -D (1.0 mg L -1 ). Maximum per cent (81.25 ± 3.6) shoot induction was achieved in MS fortified with mT (0.5 mg L -1 ) with maximum (23.67 ± 2.02) no. of shoots/explant. Maximum root (percent) induction (71.33 ± 8.3) was observed in MS medium supplemented with IBA (1.0 mg L -1 ) and maximum no. of roots/explants (10.33 ± 0.6) were observed in NAA (1.0 mg L -1 ) + Kn (0.5 mg L -1 ) fortified MS medium. Maximum per cent embryo induction (62.5 ± 7.2) was achieved in MS containing Kn (2.0 mg L -1 ) + NAA (1.0 mg L -1 ) followed by preparation of synthetic seeds in 2.5% sodium alginate and 2% CaCl 2 .

show abstract

Section: )mentioning

confidence: 71%

Organogenesis and Somatic Embryogenesis for Rapid Multiplication and Regeneration of Polygonatum Verticillatum (L.) : An Ashtavarga Medicinal Herb

Arya,

Divya,

Chaturvedi

2024

View full text Add to dashboard Cite

show abstract

“…Eapen and George (1989) reported the first finger millet plant regeneration through somatic embryogenesis. Several studies reported the use of somatic embryogenesis in finger millet (Eapen & George, 1989; Ngetich et al, 2018; Venkatesan et al, 2022), using embryoids for genetic engineering. There are successful finger millet plant regeneration using organogenesis (Mukami et al, 2018; Kansara et al, 2023.)…”

Section: Genetic Engineering and Genome Editingmentioning

confidence: 99%

Finger millet (Eleusine coracana) improvement: Challenges and prospects—A review

Gebreyohannes,

Shimelis,

Mashilo

et al. 2024

Plant Breeding

View full text Add to dashboard Cite

Finger millet is a climate‐resilient and highly nutritious small grain crop widely grown in the semi‐arid tropics. It has multiple uses, including for food, feed and beverage preparations. However, finger millet is an under‐utilized and under‐researched crop with a mean yield of <1.0 t/ha despite a potential productivity of up to 8 t/ha. The yield gap is attributed to several production constraints, such as biotic and abiotic stresses, a lack of access to improved seeds and production inputs and poor agronomic management practices. There are valuable genetic resources and genetic variability of finger millet in its centres of diversity and global gene banks for variety design, product development and commercialization. The genetic variability can be harnessed further to integrate essential traits into candidate varieties through conventional and modern breeding methods. Breeding and genetic innovations such as genomics‐assisted breeding, mutation breeding and genome editing would accelerate finger millet breeding and new variety design and deployment. The objective of this review was to document the opportunities, challenges and prospects of finger millet improvement as a guide for variety development and deployment with enhanced grain yield and nutritional contents. The first section describes global production status and yield gains, major production and productivity challenges in finger millet. This is followed by an in‐depth presentation on breeding and genetic progress on variety development with improved agronomic and nutritional quality traits, drought and salinity tolerance, and fungal diseases, weeds and insect pest resistance. Further, the review summarized finger millet's genetic and genomic resources, reference genomes, whole genome re‐sequencing and transcriptomics of finger millet technologies, genetic engineering and genome editing and their integration with conventional breeding methods for variety design with desired end‐use traits. The review provides foundational information to expedite the development of new‐generation finger millet cultivars with desirable product profiles, including high grain yield potential, early maturity, desirable seed colour, compact head type, food and feed nutrients quality and high marketability through modern breeding approaches.

show abstract

Assessing the genetic fidelity of somatic embryo-derived plantlets of finger millet by random amplified polymorphic DNA analysis

Cited by 2 publications

References 25 publications

Organogenesis and Somatic Embryogenesis for Rapid Multiplication and Regeneration of Polygonatum Verticillatum (L.) : An Ashtavarga Medicinal Herb

Organogenesis and Somatic Embryogenesis for Rapid Multiplication and Regeneration of Polygonatum Verticillatum (L.) : An Ashtavarga Medicinal Herb

Finger millet (Eleusine coracana) improvement: Challenges and prospects—A review

Contact Info

Product

Resources

About