Assessing the genetic diversity of the vir genes in Indian Plasmodium vivax population

Gupta, Purva; Das, Ashim; Singh, O. P.; Ghosh, Susanta Kumar; Singh, Vineeta

doi:10.1016/j.actatropica.2012.07.002

Cited by 11 publications

(21 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This sample was subjected to BLAST analysis whereupon it showed 99% (952/961) identity with P. vivax clone R2_21 (GenBank no. JQ733984.1) from an Indian isolate [24]. Otherwise, the difference between v21-11.27 and the Sal-1 reference gene vir 21 was 92% (882/961).…”

Section: Resultsmentioning

confidence: 99%

“…With the sequences of Indian isolates, there were common mutation with Korean isolates in T742A with M40, D5, and G1. However, D5 isolate have the only mutation in G871A [24]. Otherwise, the differences between the ROK and Sal-1 vir 4 genes were 73–79% (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…have several mechanisms for host immune evasion [33]. One of them is the pir multigene superfmily, such as kir, bir, cir , and yir families encoded by Plasmodium knowlesi , Plasmodium berghei, Plasmodium chaubadi , and Plasmodium yoelii [24,34–36]. Especially in P. falciparum , these genes already studied that encoded several functional proteins known as stevor/rif and var in P. falciparum [24].…”

Section: Discussionmentioning

confidence: 99%

“…Compared with vir 27, which belongs to subfamily I, vir 4 of subfamily C and vir 21 of subfamily B have variants with more polymorphic and distributed conserved blocks within the sequences, whereas vir 12 of subfamily E is considered to be more conserved but shown high diversity among previous studies conducted on Indian isolates [24,25]. These P. vivax sequences were amplified according to the following protocol.…”

Section: Methodsmentioning

confidence: 99%

“…Although researching the genetic variation of VSAs is critical in the development of a novel vaccine, there has been no investigation on the subject until now in the ROK. Previously, Gupta et al [7,24, 25] investigated the diversity of P. vivax populations from various regions in India with vir genes. Here, we performed a population genetic study in ROK.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation

et al. 2017

View full text Add to dashboard Cite

Variant surface antigens (VSAs) encoded by pir families are considered to be the key proteins used by many Plasmodium spp. to escape the host immune system by antigenic variation. This attribute of VSAs is a critical issue in the development of a novel vaccine. In this regard, a population genetic study of vir genes from Plasmodium vivax was performed in the Republic of Korea (ROK). Eighty-five venous blood samples and 4 of the vir genes, namely vir 27, vir 21, vir 12, and vir 4, were selected for study. The number of segregating sites (S), number of haplotypes (H), haplotype diversity (Hd), DNA diversity (π and Θw), and Tajima’s D test value were conducted. Phylogenetic trees of each gene were constructed. The vir 21 (S=143, H=22, Hd=0.827) was the most genetically diverse gene, and the vir 4 (S=6, H=4, Hd=0.556) was the opposite one. Tajima’s D values for vir 27 (1.08530, P>0.1), vir 12 (2.89007, P<0.01), and vir 21 (0.40782, P>0.1) were positive, and that of vir 4 (−1.32162, P>0.1) was negative. All phylogenetic trees showed 2 clades with no particular branching according to the geographical differences and cluster. This study is the first survey on the vir genes in ROK, providing information on the genetic level. The sample sequences from vir 4 showed a clear difference to the Sal-1 reference gene sequence, whereas they were very similar to those from Indian isolates.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation

et al. 2017

View full text Add to dashboard Cite

show abstract

In Silico Study of Variable Surface Proteins in Plasmodium Species: Perspectives in Drug Design

Yadav

Swati

2015

Interdiscip Sci Comput Life Sci

View full text Add to dashboard Cite

The variable surface proteins expressed by P. falciparum and P. vivax are transported to the surface of infected erythrocyte and are exposed to the host immune system. The possibility of using variable surface proteins as a common drug target has been analyzed in both the Plasmodium species. Sequence analysis of variable surface proteins showed a low-level conservation within as well as between the species. Amino acid composition analysis revealed higher frequency of hydrophilic amino acids as compared with that of hydrophobic residues. In order to gain more insight into their diverse functional role, the three-dimensional structure was predicted using comparative modeling approach. These models were evaluated and validated by checking stereochemistry of underlying amino acids. Structural alignment of variable surface proteins by superimposing them shows less conservation. Due to differences at sequence as well as structural level, the variable surface proteins are expected to show difference in their degree of invasiveness. These differences were also cross-examined by evolutionary study, and the results obtained were in accordance with the aforesaid study. The existence of structural differences noticed in the present study showed that the variable surface proteins could not be used as a common drug target in both the malarial species. Therefore, species-specific strategy may be followed for drug targeting against variable surface proteins of P. falciparum and P. vivax.

show abstract

Design, construction and validation of a Plasmodium vivax microarray for the transcriptome profiling of clinical isolates

et al. 2016

View full text Add to dashboard Cite

Assessing the genetic diversity of the vir genes in Indian Plasmodium vivax population

Cited by 11 publications

References 32 publications

Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation

Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation

In Silico Study of Variable Surface Proteins in Plasmodium Species: Perspectives in Drug Design

Design, construction and validation of a Plasmodium vivax microarray for the transcriptome profiling of clinical isolates

Contact Info

Product

Resources

About