Assessing the Genetic Diversity of Daylily Germplasm Using SSR Markers: Implications for Daylily Breeding

Misiukevičius, Edvinas; Frercks, Birutė; Šikšnianienė, J. B.; Kącki, Zygmunt; Gębala, Małgorzata; Akulytė, Paulina; Trilikauskaitė, Emilija; Stanys, V.

doi:10.3390/plants12091752

Cited by 4 publications

(3 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are 19 diploid and 21 tetraploid cultivars and there are different levels of genetic diversity previously described using SSR markers [15] (Supplementary Figure S1). To eliminate transplanting factors, mature daylily divisions were planted in 3-liter containers with a peat and perlite mixture (3:1) and grown for two years.…”

Section: Plant Materialsmentioning

confidence: 99%

Ploidy’s Role in Daylily Plant Resilience to Drought Stress Challenges

Misiukevičius,

Mažeikienė,

Stanys

2024

Biology

Self Cite

View full text Add to dashboard Cite

This study aimed to understand the differences in the performance of diploid and tetraploid daylily cultivars under water deficit conditions, which are essential indicators of drought tolerance. This research revealed that tetraploid daylilies performed better than diploid varieties in arid conditions due to their enhanced adaptability and resilience to water deficit conditions. The analysis of the results highlighted the need to clarify the specific physiological and molecular mechanisms underlying the enhanced drought tolerance observed in tetraploid plants compared to diploids. This research offers valuable knowledge for improving crop resilience and sustainable floricultural practices in changing environmental conditions. The morphological and physiological parameters were analyzed in 19 diploid and 21 tetraploid daylily cultivars under controlled water deficit conditions, and three drought resistance groups were formed based on the clustering of these parameters. In a high drought resistance cluster, 93.3% tetraploid cultivars were exhibited. This study demonstrates the significance of ploidy in shaping plant responses to drought stress. It emphasizes the importance of studying plant responses to water deficit in landscape horticulture to develop drought-tolerant plants and ensure aspects of climate change.

show abstract

Section: Plant Materialsmentioning

confidence: 99%

Ploidy’s Role in Daylily Plant Resilience to Drought Stress Challenges

Misiukevičius,

Mažeikienė,

Stanys

2024

Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The ploidy of an autotetraploid cv. Trahlyta was tested by flow cytometry, and its genetic identity was approved by SSR markers [40]. Plants of Hemerocallis cv.…”

Section: Plant Material Cultivation and Treatmentmentioning

confidence: 99%

Transcriptome Analysis of Diploid and Autotetraploid Hemerocallis Response to Drought Stress

Misiukevičius,

Mažeikienė,

Gossard

et al. 2023

Horticulturae

Self Cite

View full text Add to dashboard Cite

Chromosome doubling in ornamental plants, as shown by our study in daylilies (Hemerocallis spp.), has great potential to increase tolerance to abiotic stress. Drought is the most critical growth-limiting factor in a changing climate. Drought tolerance is one of the decisive factors for the survival, productivity, and appearance of perennial ornamental plants. Understanding and elucidating the molecular mechanisms that determine plant response to abiotic stress is essential. De novo transcriptome assembly of diploid and autotetraploid Hemerocallis spp. cv. Trahlyta was performed under artificially induced stress to elucidate the molecular mechanisms related to plant response to drought. In daylily mRNA, 237,886 transcripts were detected, and 42.4% of them were identified as annotated unigenes. In the experiment, diploid plants were more stressed, with 2871 upregulated or downregulated DEGs (differentially expressed genes) responding to drought, while tetraploid plants had 1599 DEGs. The proportion of upregulated DEGs differed by 1.3 times between diploid and autotetraploid genotypes, whereas the proportion of downregulated DEGs was 1.8 times greater in diploid plants. Signaling pathways related to the drought response were activated in daylilies, and key candidate genes were identified in both ploidy genotypes. In autotetraploid plants, more drought-related pathways were activated than in diploids—43 and 19, respectively. The most abundant DEGs in both cases were KEGG (Kyoto Encyclopedia of Genes and Genomes), metabolic (ko01100), and biosynthesis of secondary metabolites (ko01110) pathways. Summarizing the data, it was found that autotetraploid plants of the daylily have a wider potential for adaptation to drought stress. Therefore, they adapt faster and better to adverse drought conditions by activating alternative signaling pathways. The comparative transcriptome analysis of diploid and autotetraploid plants allows us to understand the molecular mechanisms of drought resistance and it is also essential for daylily breeding programs to develop drought-resistant genotypes in the future.

show abstract

“…Hemerocallis citrina Baroni (daylily) is a perennial herbaceous plant belonging to the Liliaceae family with edible flowers, medicinal properties, and ornamental functions. Daylilies are naturally distributed in East Asia, with the paramount diversity of species originating in Korea, Japan, and China, and have been cultivated for thousands of years ( Matand et al., 2020 ; Misiukevičius et al., 2023 ). Thrips species such as Frankliniella intonsa , Thrips palmi , and Frankliniella occidentalis are common pests of daylily, causing plant damage.…”

Section: Introductionmentioning

confidence: 99%

Physiological responses and transcriptome analysis of Hemerocallis citrina Baroni exposed to Thrips palmi feeding stress

Sun,

Shen,

Chen

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Thrips are serious pests of Hemerocallis citrina Baroni (daylily), affecting crop yield and quality. To defend against pests, daylily has evolved a set of sophisticated defense mechanisms. In the present study, induction of systemic resistance in Hemerocallis citrina ‘Datong Huanghua’ by Thrips palmi feeding was investigated at both biochemical and molecular levels. The soluble sugar content of daylily leaves was significantly lower than that in control check (CK) at all time points of feeding by T. palmi, whereas the amino acid and free fatty acid contents started to be significantly lower than those in CK after 7 days. Secondary metabolites such as tannins, flavonoids, and total phenols, which are harmful to the growth and reproduction of T. palmi, were increased significantly. The activities of defense enzymes such as peroxidase (POD), phenylalanine ammonia lyase (PAL), and polyphenol oxidase (PPO) were significantly increased, and the degree of damage to plants was reduced. The significant increase in protease inhibitor (PI) activity may lead to disrupted digestion and slower growth in T. palmi. Using RNA sequencing, 1,894 differentially expressed genes (DEGs) were identified between control and treatment groups at five timepoints. DEGs were mainly enriched in secondary metabolite synthesis, jasmonic acid (JA), salicylic acid (SA), and other defense hormone signal transduction pathways, defense enzyme synthesis, MAPK signaling, cell wall thickening, carbohydrate metabolism, photosynthesis, and other insect resistance pathways. Subsequently, 698 DEGs were predicted to be transcription factors, including bHLH and WRKY members related to biotic stress. WGCNA identified 18 hub genes in four key modules (Purple, Midnight blue, Blue, and Red) including MYB-like DNA-binding domain (TRINITY_DN2391_c0_g1, TRINITY_DN3285_c0_g1), zinc-finger of the FCS-type, C2-C2 (TRINITY_DN21050_c0_g2), and NPR1 (TRINITY_DN13045_c0_g1, TRINITY_DN855_c0_g2). The results indicate that biosynthesis of secondary metabolites, phenylalanine metabolism, PIs, and defense hormones pathways are involved in the induced resistance to T. palmi in daylily.

show abstract

Assessing the Genetic Diversity of Daylily Germplasm Using SSR Markers: Implications for Daylily Breeding

Cited by 4 publications

References 56 publications

Ploidy’s Role in Daylily Plant Resilience to Drought Stress Challenges

Ploidy’s Role in Daylily Plant Resilience to Drought Stress Challenges

Transcriptome Analysis of Diploid and Autotetraploid Hemerocallis Response to Drought Stress

Physiological responses and transcriptome analysis of Hemerocallis citrina Baroni exposed to Thrips palmi feeding stress

Contact Info

Product

Resources

About