Assessing the frequency of somatic mutation from single dog hairs—Forensic testing of StockMarks® Canine I Ver3 kit

Zenke, Petra; Egyed, Balázs; Szabolcsi, Zoltán; Zöldág, L.; Pádár, Zs.

doi:10.1016/j.fsigss.2007.10.208

Cited by 1 publication

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References 3 publications

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“…The successful analysis of mtDNA from dog hairs has been reported , however only limited data or anagen hair data from one canine individual exists for nuclear DNA STR analysis from dog hairs. Previous studies conducted on human hairs and human fingernails indicate the most likely source of directly amplified DNA is from nucleated cells or cell‐free DNA on the surface of the hair or nail .…”

Section: Sample Breakdown Of the Type Of Hair Used Per Sample Collectmentioning

confidence: 99%

Successful direct amplification of nuclear markers from single dog hairs using DogFiler multiplex

2015

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We report on successful amplification of canine STR DNA profiles from single dog hairs. Dog hairs are commonly found on clothing or items of interest in forensic casework and may be crucial associative evidence if linked to an individual dog. We used direct amplification from these hairs to increase the DNA yield of the sample, as well as greatly reducing analysis time. Hairs from different somatic regions were used from several different dog breeds to amplify a selection of eight loci from the validated DogFiler multiplex. Naturally shed canine hairs were processed, with a mix of coarse topcoat (guard) hairs and thinner soft undercoat hairs. Multiple sections of single hairs were amplified in 5 mm segments to determine the viability of DNA recovery from the shaft of the hair. Single guard hairs were cut into 5 mm sections and added directly into a PCR tube. Undercoat hairs, which are very fine, were amplified together in a single tube (approximately ten small hairs). Coarse hairs were found to be the most successful in producing full DNA profiles at all eight loci, matching the corresponding reference profile for that dog.

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Section: Sample Breakdown Of the Type Of Hair Used Per Sample Collectmentioning

confidence: 99%

Successful direct amplification of nuclear markers from single dog hairs using DogFiler multiplex

2015

View full text Add to dashboard Cite

show abstract

Assessing the frequency of somatic mutation from single dog hairs—Forensic testing of StockMarks® Canine I Ver3 kit

Cited by 1 publication

References 3 publications

Successful direct amplification of nuclear markers from single dog hairs using DogFiler multiplex

Successful direct amplification of nuclear markers from single dog hairs using DogFiler multiplex

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