Assessing the efficiency of SBA-15 as a nanocarrier for diphtheria anatoxin

Rasmussen, Martin Kaag; Bordallo, Heloisa N.; Bordenalli, Marcela Aparecida; Akamatsu, Milena Apetito; Trezena, Aryene Góes; Tino-De-Franco, Milene; Sant’Anna, Osvaldo A.; Martins, Tereza da Silva; Lopes, José Luiz de Souza; Fantini, M.C.A.; Oliveira, Cristiano L. P.

doi:10.1016/j.micromeso.2020.110763

Cited by 11 publications

(22 citation statements)

References 53 publications

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“…The focus is now shifted to SBA-15 with encapsulated dANA where no agglomerations were located in any of the particles scanned during the experiment, which is in agreement with the observations of XPCT and further confirms that dANA does not aggregate inside SBA-15. The NEXAFS spectra indicated the presence of carbon inside the particles when dANA was encapsulated in the silica, corroborating the presence of the antigen inside the mesopores of SBA-15, as also shown by TEM data (Rasmussen et al, 2021).…”

Section: Samplesupporting

confidence: 79%

“…3 (Rasmussen et al, 2021). Table 2 presents the NAI results of HBsAg and dANA inside SBA-15 (L2) for different mass ratios, confirming the higher encapsulation of the later.…”

Section: Samplementioning

confidence: 70%

“…Both were also compared with the human certified adjuvant Al(OH) 3 . These results proved the efficiency of this new formulation for oral vaccines, since a significant level of antibodies (in logarithmic scale) of oral immunization with silica are comparable with subcutaneous via and higher than those administered with Al(OH) 3 (Scaramuzzi et al, 2016;Rasmussen et al, 2021).…”

Section: Samplementioning

confidence: 72%

“…Our second goal was to conduct measurements on the diphtheria anatoxin in SBA-15 vaccine. The interest comes from the fact that diphtheria anatoxin (dANA) is much smaller, with a maximum length around 9 nm (Rasmussen et al, 2021), than HBsAg, a globular particle around 30 nm (Scaramuzzi et al, 2016), making dANA an excellent candidate for full encapsulation into SBA-15, as in principle it is small enough to enter the mesopores, having a mean mesopore diameter of around 10 nm.…”

Section: Introductionmentioning

confidence: 99%

“…(Rasmussen et al, 2021), implying that the dANA is protected inside the topical reviews IUCrJ (2022). 9Ma ´rcia Carvalho de Abreu Fantini et al Using crystallography to improve vaccines 3 of 10…”

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confidence: 99%

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Using crystallography tools to improve vaccine formulations

Fantini

Oliveira

Lopes

et al. 2021

Int Union Crystallogr J

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This article summarizes developments attained in oral vaccine formulations based on the encapsulation of antigen proteins inside porous silica matrices. These vaccine vehicles show great efficacy in protecting the proteins from the harsh acidic stomach medium, allowing the Peyer's patches in the small intestine to be reached and consequently enhancing immunity. Focusing on the pioneering research conducted at the Butantan Institute in Brazil, the optimization of the antigen encapsulation yield is reported, as well as their distribution inside the meso- and macroporous network of the porous silica. As the development of vaccines requires proper inclusion of antigens in the antibody cells, X-ray crystallography is one of the most commonly used techniques to unveil the structure of antibody-combining sites with protein antigens. Thus structural characterization and modelling of pure antigen structures, showing different dimensions, as well as their complexes, such as silica with encapsulated hepatitis B virus-like particles and diphtheria anatoxin, were performed using small-angle X-ray scattering, X-ray absorption spectroscopy, X-ray phase contrast tomography, and neutron and X-ray imaging. By combining crystallography with dynamic light scattering and transmission electron microscopy, a clearer picture of the proposed vaccine complexes is shown. Additionally, the stability of the immunogenic complex at different pH values and temperatures was checked and the efficacy of the proposed oral immunogenic complex was demonstrated. The latter was obtained by comparing the antibodies in mice with variable high and low antibody responses.

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Section: Samplesupporting

confidence: 79%

“…3 (Rasmussen et al, 2021). Table 2 presents the NAI results of HBsAg and dANA inside SBA-15 (L2) for different mass ratios, confirming the higher encapsulation of the later.…”

Section: Samplementioning

confidence: 70%

Section: Samplementioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Using crystallography tools to improve vaccine formulations

Fantini

Oliveira

Lopes

et al. 2021

Int Union Crystallogr J

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Comprehensive Review of Mesoporous Silica Nanoparticles: Drug Loading, Release, and Applications as Hemostatic Agents

Albayati,

Alardhi,

Khalbas

et al. 2024

ChemistrySelect

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Recently, mesoporous silica nanoparticles (MSNs) have emerged as promising candidates in the field of hemorrhage control owing to their extended pore size, high surface area, and excellent biocompatibility. These characteristics directly influence the toxicity of cells, the loading of therapeutic agents, and the release of active ions during the hemostasis process. Therefore, understanding the fundamentals of tuning these characteristics is important to design these types of carriers. While several literature reviews have explored the role of MSNs in hemorrhage control, comprehensive studies focusing on their general characteristics and specific applications remain scarce. This review concentrates on the principles of synthesizing mesoporous silica, the general types of MSNs, techniques for loading drugs methods onto the site of injury, release kinetics models, biocompatibility, toxicity, and the unique properties of MSNs. Furthermore, the article examines the mechanism of action of MSNs as nanomaterial hemostatic agents.

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Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A

Yang,

Jing,

Shao

et al. 2024

Nat. Prod. Bioprospect.

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Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl-γ-aminopropyl trimethoxy (R). R-SBA-15 was employed to purify and immobilize recombinant β-glucosidase from Terrabacter ginsenosidimutans (BgpA) in one step for the first time. Optimum pH of the constructed R-SBA-15@BgpA were 7.0, and it has 20 ℃ higher optimal temperature than free enzyme. Relative activity of R-SBA-15@BgpA still retained > 70% at 42 ℃ after 8-h incubation. The investigation on organic reagent resistance revealed that the immobilized enzyme can maintain strong stability in 15% DMSO. In leaching test and evaluation of storage stability, only trace amount of protein was detected in buffer of the immobilized enzyme after storage at 4 ℃ for 33 days, and the immobilized BgpA still maintained > 50% relative activity. It also demonstrated good reusability, with 76.1% relative activity remaining after fourteen successive enzymatic hydrolyses of epimedin A to sagittatoside A. The newly proposed strategy is an effective approach for the purification and immobilization of BgpA concurrently. In addition, R-SBA-15@BgpA was demonstrated to have high efficiency and stability in this application, suggesting its great feasibility and potential to produce bioactive compounds such as secondary glycosides or aglycones from natural products. Graphical Abstract

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Assessing the efficiency of SBA-15 as a nanocarrier for diphtheria anatoxin

Cited by 11 publications

References 53 publications

Using crystallography tools to improve vaccine formulations

Using crystallography tools to improve vaccine formulations

Comprehensive Review of Mesoporous Silica Nanoparticles: Drug Loading, Release, and Applications as Hemostatic Agents

Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A

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