Assessing the Efficacy of <i>Bacillus thuringiensis</i> (Bt) Pyramided Proteins Cry1F, Cry1A.105, Cry2Ab2, and Vip3Aa20 Expressed in Bt Maize Against Lepidopteran Pests in Brazil

Marques, Luiz Henrique da Silva Fagundes; Santos, Antonio Carlos; Castro, B. A.; Moscardini, Valéria Fonseca; Rosseto, Jaedino; Silva, Oscar A.B.N.; Babcock, Jon

doi:10.1093/jee/toy380

Cited by 20 publications

(16 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the experiences in North and South America (Huang et al ., ; Burtet et al ., ) and South Africa (Botha et al ., ), Bt corn plants can be highly effective on S. frugiperda when utilized as part of an IPM program after registration for cultivation in China in the future. The use of pyramided traits expressing effective Bt proteins on S. frugiperda with different sites of action (Burtet et al ., ; Marques et al ., ) should be prioritized and then deployed with appropriate refuge, resistance monitoring, and grower education. In southern China, three similar noctuid larvae of native lepidopteran species ( S. litura , S. exigua and M. separata ) are often found in the same field and each species can cause considerable damage on important agricultural crops, such as corn, rice, wheat, cotton and vegetables (Jiang et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques

Jing

Guo

Jiang³

et al. 2019

Insect Science

149

109

View full text Add to dashboard Cite

The fall armyworm, Spodoptera frugiperda, is a species native to the Americas and has spread to many countries in Africa and Asia in recent years. Proactive actions for potential invasion of S. frugiperda to China coordinated by government agencies and agricultural extension systems resulted in timely detection in January 2019 in Yunnan province neighboring onto Myanmar. The extensive monitoring in southern provinces of China since February 2019 resulted in dynamic tracking of S. frugiperda spreading to 13 provincial regions in China within 4 months by May 10, 2019, which is crucial for timely management actions in the fields. The first detections of S. frugiperda (corn strain) in China were confirmed using cytochrome oxidase subunit 1 (CO1) and triosephosphate isomerase (Tpi) genes molecular marker method. In addition to S. frugiperda, larvae of three other noctuid species with similar morphological appearance (S. litura, S. exigua and Mythimna separata) can occur simultaneously and cause similar damage in cornfields in southern China. Thus, we can use both morphological and molecular marker methods to compare larval stages of four noctuid species. Further, we discuss the risk of potential spread of invasive S. frugiperda to other regions and impact on corn production in China.

show abstract

Section: Discussionmentioning

confidence: 99%

Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques

Jing

Guo

Jiang³

et al. 2019

Insect Science

149

109

View full text Add to dashboard Cite

show abstract

“…showed injury from E. lignosellus whereas 66.5% of non-transgenic maize suffered injury from E. lignosellus (Marques et al, 2019).…”

Section: Impactsmentioning

confidence: 98%

“…These climates also occur in the EU. E. lignosellus have also been found in climates Csa (USDA, 1953(USDA, , 1961, Csb (Luginbill and Ainslie, 1917), Cfb (Marques et al, 2019) and BSk (Reynolds et al, 1959). These climate types also occur in the EU (Figure 3) (MacLeod and Korycinska, 2019).…”

Section: Climatic Conditions Affecting Establishmentmentioning

confidence: 99%

“…The widespread use of transgenic maize in the USA and Brazil limits the impact of E. lignosellus (Buntin et al., 2004) ; seedlings of transgenic maize suffer significantly lower feeding damage from E. lignosellus than non‐transgenic maize seedlings (Vilella et al., 2002). In trials in Brazil, less than 5% of transgenic maize showed injury from E. lignosellus whereas 66.5% of non‐transgenic maize suffered injury from E. lignosellus (Marques et al., 2019).…”

Section: Pest Categorisationmentioning

confidence: 99%

See 1 more Smart Citation

Pest categorisation of Elasmopalpus lignosellus

Bragard¹,

Dehnen‐Schmutz²,

Serio³

et al. 2021

EFS2

View full text Add to dashboard Cite

The European Commission requested the EFSA Panel on Plant Health to conduct a pest categorisation of Elasmopalpus lignosellus (Zeller) (Leipidoptera: Pyralidae) for the territory of the EU following interceptions of the organism within the EU and its addition to the EPPO Alert List. E. lignosellus feeds on over 70 species; hosts include cereals, especially maize, legumes, brassicas and a range of grasses. Seedlings of ornamental and forest trees can also be hosts. E. lignosellus is established in tropical and subtropical areas of North, Central and South America. Eggs are usually laid in the soil or on the lower stem of hosts. Larvae develop in the soil and feed on roots and stems causing stunting and yield losses. Plants for planting, rooted with growing media, or with stems cut close to the soil, and fresh vegetables harvested with stems, such as asparagus and cabbage, provide pathways for entry. Population development is favoured by dry and hot conditions (27–33°C). Adults fly and can be carried in air currents. Adults are recorded from temperate areas within the Americas contributing some uncertainty regarding the limits of its establishment potential in the EU . Although cultivated and wild hosts are distributed across the EU , impacts are likely to be confined to production areas on sandy soils around the coastal Mediterranean during hot dry years. Phytosanitary measures are available to inhibit the entry of E. lignosellus. E. lignosellus satisfies the criteria that are within the remit of EFSA to assess for it to be regarded as a potential Union quarantine pest.

show abstract

“…Among the maize transgenic technologies, hybrids containing PowerCore Ultra (events DAS-Ø15Ø7-1 × MON-89Ø34-3 × MON-ØØ6Ø3–6 × SYN-IR162-4) express 2 proteins for herbicide tolerance (PAT and CP4 EPSPS) and 4 insecticidal Bt proteins (Cry1F, Cry1A.105, Cry2Ab2, and Vip3Aa20) targeting the insect pests Diatraea saccharalis (F.) (Lepidoptera: Crambidae), Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), Elasmopalpus lignosellus (Zeller) (Lepidoptera: Pyralidae), Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) and the fall armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) ( Marques et al 2019 , Moscardini et al 2020 ). The fall armyworm is considered one of the most important pests in South America due to its polyphagous habit, high reproductive rate, migratory behavior and high phenotypic plasticity which allows this species to feed on different hosts as well as develop resistance to multiple control tactics ( Nagoshi et al 2015 , Blanco et al 2016 , Silva-Brandão et al 2017 , Okuma et al 2018 , Arias et al 2019 ).…”

mentioning

confidence: 99%

Water Deprivation Induces Biochemical Changes Without Reduction in the Insecticidal Activity of Maize and Soybean Transgenic Plants

Silva

Mori

Marques³

et al. 2021

Journal of Economic Entomology

View full text Add to dashboard Cite

Like conventional crops, transgenic plants expressing insecticidal toxins from Bacillus thuringiensis (Bt) are subjected to water deprivation. However, the effects of water deprivation over the insecticidal activity of Bt plants are not well understood. We submitted Bt maize and Bt soybean to water deprivation and evaluated biochemical stress markers and the insecticidal activity of plants against target insects. Bt maize (DAS-Ø15Ø7-1 × MON-89Ø34-3 × MON-ØØ6Ø3–6 × SYN-IR162-4) containing the PowerCore Ultra traits, Bt soybean (DAS-444Ø6-6 × DAS-81419-2) with the Conkesta E3 traits, and commercial non-Bt cultivars were cultivated and exposed to water deprivation in the greenhouse. Leaves were harvested for quantification of hydrogen peroxide, malondialdeyde (MDA), and total phenolics and insecticidal activity. Maize or soybean leaf disks were used to evaluate the insecticidal activity against, respectively, Spodoptera frugiperda (J.E Smith) and Chrysodeixis includens (Walker) neonates. Except for Bt soybean, water deprivation increased hydrogen peroxide and MDA contents in Bt and non-Bt plants. Both biochemical markers of water deficit were observed in lower concentrations in Bt plants than in non-Bt commercial cultivars. Water deprivation did not result in changes of phenolic contents in Bt and non-Bt maize. For Bt or non-Bt soybean, phenolic contents were similar despite plants being exposed or not to water deprivation. Water deprivation did not alter substantially insect survival in non-Bt maize or non-Bt soybean. Despite water deprivation-induced biochemical changes in plants, both Bt plants maintained their insecticidal activity (100% mortality) against the target species.

show abstract

Assessing the Efficacy of Bacillus thuringiensis (Bt) Pyramided Proteins Cry1F, Cry1A.105, Cry2Ab2, and Vip3Aa20 Expressed in Bt Maize Against Lepidopteran Pests in Brazil

Cited by 20 publications

References 37 publications

Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques

Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques

Pest categorisation of Elasmopalpus lignosellus

Water Deprivation Induces Biochemical Changes Without Reduction in the Insecticidal Activity of Maize and Soybean Transgenic Plants

Contact Info

Product

Resources

About