Assessing the effects of silver nanoparticles on monolayers of differentiated Caco-2 cells, as a model of intestinal barrier

Vila, Laura; García‐Rodríguez, Alba; Cortés, Constanza; Marcos, Ricard; Hernández, Alba

doi:10.1016/j.fct.2018.04.008

Cited by 51 publications

(44 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using the atomic absorption spectroscopy (AAS) analysis, the authors were able to detect an average of 10 pg/cell of 50 nm uncoated AgNPs. This would confirm our recent studies in Caco-2 cells where AgNPs induced DNA strand breaks in differentiated cells, mediated by oxidative DNA damage [56], increasing a wide set of cell-transformation biomarkers in long-term exposed undifferentiated cells [57]. The induction of MN by AgNPs has been attributed to size, with MN induction at the small size (20 nm) in both Caco-2 and HepG2 cells but only MN induction in HepG2 cells at a larger size (50 nm) [21,23].…”

Section: Fcmn Assay Cbmn Assaysupporting

confidence: 92%

Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials

et al. 2019

Self Cite

View full text Add to dashboard Cite

Thousands of nanomaterials (NMs)-containing products are currently under development or incorporated in the consumer market, despite our very limited understanding of their genotoxic potential. Taking into account that the toxicity and genotoxicity of NMs strongly depend on their physicochemical characteristics, many variables must be considered in the safety evaluation of each given NM. In this scenario, the challenge is to establish high-throughput methodologies able to generate rapid and robust genotoxicity data that can be used to critically assess and/or predict the biological effects associated with those NMs being under development or already present in the market. In this study, we have evaluated the advantages of using a flow cytometry-based approach testing micronucleus (MNs) induction (FCMN assay). In the frame of the EU NANoREG project, we have tested six different NMs—namely NM100 and NM101 (TiO2NPs), NM110 (ZnONPs), NM212 (CeO2NPs), NM300K (AgNPs) and NM401 (multi-walled carbon nanotubes (MWCNTs)). The obtained results confirm the ability of AgNPs and MWCNTs to induce MN in the human bronchial epithelial BEAS-2B cell line, whereas the other tested NMs retrieved non-significant increases in the MN frequency. Based on the alignment of the results with the data reported in the literature and the performance of the FCMN assay, we strongly recommend this assay as a reference method to systematically evaluate the potential genotoxicity of NMs.

show abstract

Section: Fcmn Assay Cbmn Assaysupporting

confidence: 92%

Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…This is supported by other works in which the toxicity of silver nanoparticles is usually in the range of 10 to 100 µg [93]. It was observed in the work of Vila et al [87] that the exposure of small-sized Ag-NPs (≈ 8 nm) at a concentration of 100 µg/mL only reached 20% cytotoxicity in Caco-2 cells. It was also shown that cell integrity was not altered using concentrations below 50 µg/mL.…”

Section: In Vitro Studies: Static and Dynamic Gut Simulators And Epitsupporting

confidence: 74%

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

et al. 2020

View full text Add to dashboard Cite

Foodborne diseases are one of the factors that endanger the health of consumers, especially in people at risk of exclusion and in developing countries. The continuing search for effective antimicrobials to be used in the food industry has resulted in the emergence of nanotechnology in this area. Silver nanoparticles (Ag-NPs) are the nanomaterial with the best antimicrobial activity and therefore, with great potential of application in food processing and packing. However, possible health effects must be properly addressed to ensure food safety. This review presents a detailed description on the main applications of Ag-NPs as antimicrobial agents for food control, as well as the current legislation concerning these materials. Current knowledge about the impact of the dietary exposure to Ag-NPs in human health with special emphasis on the changes that nanoparticles undergo after passing through the gastrointestinal tract and how they alter the oral and gut microbiota, is also summarized. It is concluded that given their potential and wide properties against foodborne pathogens, research in Ag-NPs is of great interest but is not exempt from difficulties that must be resolved in order to certify the safety of their use.

show abstract

“…Observational studies in which increased blood levels of TiO 2 NPs were found in patients with ulcerative colitis compared to healthy individuals provide further evidence for the enhanced translocation of NPs across a compromised intestinal barrier . There is evidence, however, that not all NPs may have this effect, as the administration of Ag NPs to a Caco‐2 monolayer in vitro showed no loss of barrier integrity …”

Section: Nps and The Intestinal Barriermentioning

confidence: 99%

Nanoparticles and danger signals: Oral delivery vehicles as potential disruptors of intestinal barrier homeostasis

Vita

Royse

Pullen

2019

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Gut immune system homeostasis involves diverse structural interactions among resident microbiota, the protective mucus layer, and a variety of cells (intestinal epithelial, lymphoid, and myeloid). Due to the substantial surface area in direct contact with an "external" environment and the diversity of xenobiotic, abiotic, and self-interactions coordinating to maintain gut homeostasis, there is enhanced potential for the generation of endogenous danger signals when this balance is lost. Here, we focus on the potential generation and reception of damage in the gut resulting from exposure to nanoparticles (NPs), common food and drug additives. Specifically, we describe recent evidence in the literature showing that certain NPs are potential generators of damage-associated molecular patterns, as well as potential immune-stimulating molecular patterns themselves. K E Y W O R D SATP, DAMP, mtDNA, NAMP, Nanoparticles, NLRP3Over the past decade, the commercial use of NPs by the food and biomedical industries has seen immense growth. The small, nanoscale size of NPs confers unique physicochemical properties that allow them to be effective oral delivery mechanisms for pharmaceuticals, food flavors, nutrient additives, and more. The accepted size range of NPs is not straightforward, varying across different biomedical and engineering disciplines and government agencies. 10,11 Although certain sources indicate that a typical NP is 1-100 nm in three dimensions, other sources consider accepted dimensions (one or more) for NPs to include <200 nm, 12 <500 nm, 13 or as large as <1000 nm. 14,15 The ASTM International definition provides for either two or three dimensions at the nanoscale, 16 whereas the ISO definition indicates nanoscale in three dimensions. 17 In an officially issued

show abstract

Assessing the effects of silver nanoparticles on monolayers of differentiated Caco-2 cells, as a model of intestinal barrier

Cited by 51 publications

References 45 publications

Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials

Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

Nanoparticles and danger signals: Oral delivery vehicles as potential disruptors of intestinal barrier homeostasis

Contact Info

Product

Resources

About