Assessing the effects of Irgarol 1051 on marine phytoplankton populations in Key Largo Harbor, Florida

Zamora-Ley, Ingrid M.; Gardinali, Piero R.; Jochem, Frank J.

doi:10.1016/j.marpolbul.2005.12.010

Cited by 21 publications

(14 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A few studies involving flow cytometry reported the toxicity of Irgarol 1051 Ò on phytoplankton communities (Devilla et al 2005;Readman et al 2004;Zamora-Ley et al 2006). …”

Section: Flow Cytometrymentioning

confidence: 99%

See 1 more Smart Citation

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Stachowski-Haberkorn

Quiniou²,

Beker³

et al. 2008

Ecotoxicology

View full text Add to dashboard Cite

The impacts of the fungicide Opus (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 microg l(-1) of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 microg l(-1), TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant differences in microcosms exposed to 10 microg l(-1) of epoxiconazole. When exposed to 100 microg l(-1), all three methods allowed to detect significant differences in treated microcosms, as compared to controls. The TTGE analysis appears in this study as the most sensitive method for fungicide impact assessment on eukaryote microbial communities.

show abstract

“…A few studies involving flow cytometry reported the toxicity of Irgarol 1051 Ò on phytoplankton communities (Devilla et al 2005;Readman et al 2004;Zamora-Ley et al 2006). …”

Section: Flow Cytometrymentioning

confidence: 99%

“…Toxicity assessment using flow cytometry was reported in studies involving either phytoplankton cultures (Lage et al 2001;Stauber and Adams 2005;Yu et al 2007) or natural photosynthetic communities (de la Broise and Palenik 2007; Readman et al 2004;Stachowski-Haberkorn et al 2008;Zamora-Ley et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Stachowski-Haberkorn

Quiniou²,

Beker³

et al. 2008

Ecotoxicology

View full text Add to dashboard Cite

show abstract

“…Studies have reported Irgarol in waters and sediments in the vicinity of coral reefs (e.g. Sheihk et al 2009;Kitada et al 2008;Zamora-Ley et al 2006;Carbery et al 2006). At high waterborne concentrations of Irgarol (30 lg/l), a 30% reduction in coral zooxanthellae density was demonstrated in Seriatopora hystrix (Jones and Kerswell 2003).…”

Section: Introductionmentioning

confidence: 99%

Concentrations of Irgarol in selected marinas of Oahu, Hawaii and effects on settlement of coral larval

Knutson

Downs²,

Richmond³

2011

Ecotoxicology

View full text Add to dashboard Cite

This study examined concentrations of Irgarol 1051(®) in selected marinas on the island of Oahu, Hawaii and used laboratory bioassays to assess effects of Irgarol on coral larval settlement. Field surveys of small boat marinas performed in 2006-2007 revealed low concentrations of Irgarol 1051(®), an antifouling paint additive, ranging from non-detected (<17 ng/l) to 283 ng/l. The highest concentrations of Irgarol 1051(®) were found in marinas with low flushing rates and a high density of moored boats and boat traffic. The potential effect of Irgarol 1051(®) on coral larval settlement was evaluated in the laboratory using planulae from Porites hawaiiensis, a zooxanthellate shade-dwelling coral found in Hawaiian waters. Exposure to Irgarol 1051(®) at 100 ng/l resulted in a statistically significant reduction in settlement of coral larvae. This was within the range of Irgarol 1051(®) concentrations found in some of the marinas surveyed on the island of Oahu but Irgarol was not detected in seawater samples at offshore reefs.

show abstract

“…Flow cytometry can also provide information about the distribution and abundance of natural photosynthetic populations (Marie et al 1999;Rutten et al 2005). Toxicity assessment using flow cytometry has been used both in studies based on phytoplankton cultures (Lage et al 2001;Stauber et al 2005;Yu et al 2007) and those examining natural photosynthetic communities (Readman et al 2004;Zamora-Ley et al 2006;de la Broise and Palenik 2007;Stachowski-Haberkorn et al 2008. These two complementary techniques were combined in our sample analysis to increase the sensitivity with which we could compare phytoplankton communities.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the partial renewal of in situ phytoplankton microcosms and application to the impact assessment of bentazon and dimethenamid

Broise

Stachowski-Haberkorn

2012

Marine Pollution Bulletin

View full text Add to dashboard Cite

Microcosms, each consisting of 2L natural surface seawater maintained in 2.3-L glass bottles, were immersed at a depth of 6m. The renewal of 10% of microcosm volumes was carried out every other day. Phytoplankton-containing seawater was used for renewal (previously filtered through 25-, 50- or 200-μm cut-off). Phytoplankton community pigment analysis (by HPLC) and flow cytometry analysis were performed. After 13 days, data exhibited phytoplankton characteristics in microcosms in the same range as that of the natural surrounding sea water over the same period. Furthermore, in these microcosms, a negative correlation was observed between the filtration cut-off used for renewal water, and the total cell count. Herbicides were tested as commercial mixtures at 1, 10 and 100 μgL(-1) active substance. Both Frontier® (dimethenamid) and Basamais® (bentazon) induced significant modifications of the phytoplankton populations at every concentration tested. Such results suggest a possible disturbance in polluted coastal areas.

show abstract

Assessing the effects of Irgarol 1051 on marine phytoplankton populations in Key Largo Harbor, Florida

Cited by 21 publications

References 23 publications

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Concentrations of Irgarol in selected marinas of Oahu, Hawaii and effects on settlement of coral larval

Evaluation of the partial renewal of in situ phytoplankton microcosms and application to the impact assessment of bentazon and dimethenamid

Contact Info

Product

Resources

About