Assessing the effects of exposure to environmental stress on some functional properties of Bifidobacterium animalis ssp. lactis

Abstract

This study assessed the effects of exposing a strain of Bifidobacterium animalis ssp. lactis to acid, bile and osmotic stresses on antagonistic properties, biofilm formation and antibiotic susceptibility/resistance profile. Exposure to each stress factor appeared to have no significant effect on the antagonism against Escherichia coli NCTC 12900 and Salmonella enterica serovar Enteritidis PT4. No suppression in biofilm formation due to exposure to stress was observed. Bile and osmotic stresses resulted in significantly higher biofilm formation. Expression of an exopolysaccharide synthesis gene, gtf 01207, was significantly higher when the B. animalis ssp. lactis strain was exposed to osmotic stress. Susceptibility of the B. animalis ssp. lactis strain to chloramphenicol, erythromycin, ampicillin and vancomycin, and resistance to tetracycline remained unchanged when exposed to each stress. The expression of a tetracycline resistance gene, tet(W), was significantly higher when exposed to each stress. These results may suggest that the potential for the B. animalis ssp. lactis strain to provide probiotic benefit, after exposure to the stressful conditions of the gastrointestinal tract, remains intact.

Exploring the relationship between exposure to technological and gastrointestinal stress and probiotic functional properties of lactobacilli and bifidobacteria

“…lactis in the presence of bile (Ruas-Madiedo et al 2009). Amund et al (2014) also showed higher expression of gtf01207 in B. animalis subsp. lactis after exposure to acid, bile, and osmotic stresses, though only significantly for osmotic stress.…”

“…Tolerance of probiotics to therapeutic doses of antibiotics may be desirable when probiotics are coadministered with antibiotics to prevent antibioticassociated diarrhoea (Amund et al 2014). Few studies have examined the effects of exposure to gastrointestinal stress on the susceptibility of lactobacilli and bifidobacteria to antibiotics.…”

“…This may be due to modified surface properties and cell permeability. Amund et al (2014) reported higher expression of the tetracycline resistance gene tet(W) in Bifidobacterium animalis subsp. lactis after exposure to acid, bile, and osmotic stress conditions.…”

“…Higher expression of tet(W) in B. animalis subsp. lactis after exposure to stresses did not however manifest as greater tetracycline resistance (Amund et al 2014). The tet genes code for proteins that protect the ribosomes, which are the target for tetracyclines (Gueimonde et al 2010).…”

Functional Gastrointestinal Disorders in Infants: Causes of Occurrance, Diagnostic Criteria and Possibilities of Correction With Probiotics

“…Установлено, что данный пробиотический штамм: ■ способен к высокоуровневой адгезии к поверхностям с муцином, без муцина и пленкам клеточных культур [34]; ■ устойчив к действию агрессивных сред организма че-ловека -соляной кислоты и желчи, за счет синтеза рН-зависимой АТФ-азы, регулирующей кислотно-основ-ной баланс внутри бактерии и наличия гидролазы солей желчных кислот, позволяющей бактерии сохранять актив-ность в присутствии желчи [35] [36]; и резистентностью к таким антибиотикам, как гентамицин, стреп-томицин, полимиксин В, канамицин, нео мицин, циклосерин, тетрациклин и налидиксовая кислота [37] -способ-ствует улучшению барьерной функции кишечного эпителия (повышение кон-центрации секреторного IgA) [38,39] и положительно влияет на работу иммун-ной системы [40].…”