Assessing the effectiveness of a herbal mouthwash against oral pathogens: analysis

Abstract

The most common infectious diseases caused by numerous viruses and bacteria include dental caries and periodontal diseases, which can develop at different stages of a person's life. Herbal mouthwash is highly promising because of its positive effects and works on oral infections and bacteria to quickly cure pain. In this study, a herbal mouthwash with a range of plant extracts was prepared. Medicinal plants like Azadirachta indica, Ocimum tenuiflorum, and Clincanthus nutans was used. Their usefulness in halting the growth of harmful bacterial species such Staphylococcus sp was evaluated. Clinacanthus nutans has strong antibacterial properties and non- toxic to cell lines. Phytochemical was carried out and characterization analysis such as FTIR and GC-MS analysis were performed to identify the function groups and important constituents. In vivo toxicity analysis was performed and the LC50 value for the three formulations A, B, C were found to be 16.0598 µg/ml,15.948 µg/ml,16.058 µg/ml respectively.The physical characteristics such as pH, colour, and stability, have been assessed. Antibacterial activity against an oral sample on blood agar showed good zone of inhibition for three different formulations. The formulation C showed maximum inhibition of 25mm for 100µl concentration of the sample.