Assessing the effect of selection with deltamethrin on biological parameters and detoxifying enzymes in <scp><i>Aedes aegypti</i></scp> (L.)

Alvarez‐Gonzalez, Leslie C; Briceño, Arelis; Ponce‐García, Gustavo; Villanueva-Segura, Olga Karina; Davila-Barboza, Jesus A.; López-Monroy, Beatriz; Gutierrez-Rodriguez, Selene M; Contreras‐Perera, Yamili; Rodríguez‐Sánchez, Iram P.; Flores, Adriana E.

doi:10.1002/ps.4609

Cited by 20 publications

(22 citation statements)

References 34 publications

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“…In the absence of insecticide applications, resistance alleles can result in energetic costs or fitness disadvantages in comparison with its susceptible counterparts. Attempts to register fitness costs due to insecticide resistance are often based on the selection of insects in the laboratory for increased resistance or on backcrosses with laboratory strains to produce lineages differing only in the resistance traits [ 11 , 28 – 30 , 48 , 49 ]. This approach supposedly enables more accurate measures of changes on the fitness parameters related specifically to insecticide resistance rather than other genetic differences.…”

Section: Discussionmentioning

confidence: 99%

Insecticide Resistance and Fitness: The Case of Four Aedes aegypti Populations from Different Brazilian Regions

David

Garcia

Valle

et al. 2018

BioMed Research International

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Background Chemical control is still a major strategy to constrain vector density and mitigate pathogen transmission. However, insecticide overuse poses a high selective pressure, favouring the spread of resistance alleles in natural populations. In an insecticide-free environment, a fitness cost is expected in resistant insects when compared to susceptible counterparts. This study investigates whether insecticide resistance to an organophosphate (temephos) and a pyrethroid (deltamethrin) is associated with fitness traits in four Aedes aegypti wild populations sampled every three months over one year. Findings We measured development time from larvae to adult, female survival, wing length, fecundity, and adult resistance to starvation in field insecticide resistant Ae. aegypti populations four times over a year. These results were confronted with resistance levels to temephos and deltamethrin and with potentially related mechanisms, including a kdr mutation in the pyrethroid target site. No differences in fitness cost were found after contrasting mosquitoes from the same population collected throughout a year, irrespective of differences in insecticide resistance levels. Additionally, significant differences were not observed among field populations. However, compared to the reference strain Rockefeller, field females survived significantly less. Moreover, larval development was equal or slower in three out of four field populations. In no case differences were evidenced in starvation tolerance, wing length, and fecundity. Conclusions Overall, field resistant mosquitoes seemed to have a slight fitness disadvantage when compared with the Rockefeller susceptible strain which might represent a potential fitness cost of insecticide resistance. However, after comparing Ae. aegypti from the same population but sampled at different moments, or from different field populations, mosquito life-history traits varied independently of resistance ratios. The metabolic deviations necessary to overcome the adverse effects of insecticides may cause an energy trade-off that affects energy allocation and ultimately basic demands of insect biology. The extent of fitness cost due to insecticide resistance is critical information to delay the evolution of resistance in wild vector populations.

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Section: Discussionmentioning

confidence: 99%

Insecticide Resistance and Fitness: The Case of Four Aedes aegypti Populations from Different Brazilian Regions

David

Garcia

Valle

et al. 2018

BioMed Research International

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“…Interestingly, ♀:♂ of the CRR was higher in � 21 d than in > 21 d, but of the CSS was no obvious difference between in � 21 d and > 21 d (S3 and S4 Tables). The other pyrethroids resistance studies in Musca domestica and Aedes aegypti showed an obvious fitness costs but no effects on sex ratio [14,33]. However, in the CRR, ♀:♂ was lower no matter in � 21 or � 30 d life table in comparison with the ones in the CSS and also appeared an age-specific reduction (Figs 1 and 2 Table), which meant beta-cypermethrin resistance caused a significant effect on sex ratio in houseflies.…”

Section: Fig 2 Comparisons Of the Life-history Trait Values Between mentioning

confidence: 84%

“…A lambda-cyhalothrin-resistant strain of houseflies showed only 26% fitness in comparison with that of susceptible strains [14]. Under deltamethrin selection pressures, Aedes aegypti fitness decreased by 41% [33]. Diflubenzuron resistance in an Aedes aegypti field population was associated with a fitness costs [34].…”

Section: Discussionmentioning

confidence: 95%

“…In the Vip3Aa20-resistant strain of Spodoptera frugiperda, autosome-recessive and monogenic � 30 d The leading cause of fitness costs associated with beta-cypermethrin resistance in housefly resistance resulted in a reduction in the survival rate by 11% until the adult stage and an~50% lower reproductive rate in comparison with those in susceptible and heterozygous strains [36]. Under deltamethrin selection, the total fertility, survival and reproductive rate were reduced and intrinsic growth rate also declined in Aedes aegypti, which was associated with the presence of recessive alleles of the V1016I and F1534C mutations [33]. In our study on houseflies, The leading cause of fitness costs associated with beta-cypermethrin resistance in housefly the lower survival rate and obvious decreases in r m and population size observed in the CRR are very likely due to the mutation (Trp251-Ser) of the carboxylesterase gene MdαE7 under the strong resistance selection pressure caused by beta-cypermethrin, the resistance to which is inherited as a single, major, autosomal and incompletely recessive factor [9,10].…”

Section: Discussionmentioning

confidence: 99%

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Role transformation of fecundity and viability: The leading cause of fitness costs associated with beta-cypermethrin resistance in Musca domestica

Shi

Zhang

et al. 2020

PLoS ONE

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Fitness is closely associated with the development of pesticide resistance in insects, which determines the control strategies employed to target species and the risks of toxicity faced by non-target species. After years of selections with beta-cypermethrin in laboratory, a strain of housefly was developed that was 684,521.62-fold resistant (CRR) compared with the susceptible strain (CSS). By constructing � 21 d and � 30 d life tables, the differences in life history parameters between CSS and CRR were analyzed. The total production numbers of all the detected development stages in CRR were lower than in CSS. Except for the lower mortality of larvae, all the other detected mortalities in CRR were higher than in CSS. ♀:♂ and normal females of CRR were also lower than those of CSS. For CRR, the relative fitness was 0.25 in the � 21 d life table and 0.24 in the � 30 d life table, and a lower intrinsic rate of increase (r m ) and net reproductive rate (R o ) were detected. Based on phenotype correlation and structural equation model (SEM) analyses, fecundity and viability were the only directly positive fitness components affecting fitness in CRR and CSS, and the other components played indirect roles in fitness. The variations of the relationships among fitness, fecundity and viability seemed to be the core issue resulting in fitness differences between CRR and CSS. The interactions among all the detected fitness components and the mating frequency-time curves appeared to be distinctly different between CRR and CSS. In summary, fecundity and its related factors separately played direct and indirect roles in the fitness costs of a highly beta-cypermethrin-resistant housefly strain. PLOS ONE | https://doi.org/10.1371/journal.pone.0228268 January 30, 2020 1 / 18 OPEN ACCESS Citation: Shi J, Zhang L, Mi J, Gao X (2020) Role transformation of fecundity and viability: The leading cause of fitness costs associated with betacypermethrin resistance in Musca domestica. PLoS ONE 15(1): e0228268. https://doi.org/ 10.

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“…Resistance to pyrethroids in mosquitoes have been widely associated with the F1534C (21, 23, 25, 27, 29, 30, 32, 39–41, 83, 88–101) and V1016I mutations (24, 25, 27, 29, 30, 32, 36, 38, 40, 41, 88, 93, 97, 100, 102, 103). The simultaneous presence of both mutations has been associated with enhanced tolerance to deltamethrin in the past (31).…”

Section: Discussionmentioning

confidence: 99%

The need to harmonize insecticide resistance testing: methodology, intensity concentrations and molecular mechanisms evaluated inAedes aegyptipopulations in Central America and Hispaniola

Ledoux

Gutiérrez

Lobo

et al. 2020

Preprint

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BackgroundThe Zika AIRS Project, a USAID-funded initiative worked across the Latin America and Caribbean regions from 2016 to 2019, as an emergency to contain the spread of the Zika virus. All entomological records in the target countries showed wide distribution and high abundance of Aedes aegypti populations, however the susceptibility profiles of these insects to insecticides commonly employed by vector control campaigns were in most cases incomplete or inexistent. In close collaboration with the Ministries of Health of individual countries, Zika-AIRS teams conducted insecticide susceptibility testing of an array of insecticides in A. aegypti populations of each country. Procedures applied met the standard international protocols instructed by the World Health Organization and Centers for Disease Control and Prevention.Methodology and main findingsThe insecticides tested were selected under categories such as pyrethroids, organophosphates and carbamate. Results showed A. aegypti populations displaying high and widely distributed resistance to all pyrethroids across countries, tolerance to organophosphates and full susceptibility to a carbamate. Key inconsistencies between testing methods are presented and discussed. Additionally, four kdr mutations were analyzed to detect molecular mechanisms of insecticide resistance. The screening for kdr mutations suggested the widespread nature of V1016I mutation, linked to pyrethroid resistance in A. aegypti populations distributed and sampled in the above mentioned regions.Conclusions and perspectivesThis multi-country study contributes with updated information to the public health decision-makers across Central America and the Caribbean. This study provided training and established technical networks for more effective and sustainable insecticide surveillance programs. Most but not all records of insecticide resistance in A. aegypti were consistent between methodologies, thus inconsistent issues are discussed here to call for further improvement in procedures and convey more practical guidelines for surveillance teams in countries where Aedes-borne diseases are endemic.Author summaryAt the forefront of the fight against arboviruses transmission is the insecticide-based vector control. All countries in the Latin American and Caribbean region invest valuable resources from their limited budget to acquire and implement insecticide-based tools, with non-existent or weak insecticide resistance monitoring programs. Hence, the USAID-funded Zika AIRS Project (ZAP) collaborated with the Ministries of Health of multiple countries to update the profile of susceptibility to insecticides in Aedes aegypti populations. We found widespread resistance to pyrethroid and organophosphate insecticides, which account to almost 100% of the products available to control adult mosquitoes. As we used both of World Health Organization and Centers for Disease Control and Prevention standard methods, we found many similarities and some inconsistencies in the susceptibility profiles obtained for the very same vector populations. Additionally, we obtained insight on potential molecular mechanisms of resistance across the countries, finding the kdr mutation V1016I possibly involved in loss of susceptibility.This study is the biggest cross-country update of insecticide resistance for Aedes aegypti in years, and it should be used as evidence for improving the selection of insecticides in these countries and a call for further support to maintain insecticide resistance monitoring programs.

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Assessing the effect of selection with deltamethrin on biological parameters and detoxifying enzymes in Aedes aegypti (L.)

Cited by 20 publications

References 34 publications

Insecticide Resistance and Fitness: The Case of Four Aedes aegypti Populations from Different Brazilian Regions

Insecticide Resistance and Fitness: The Case of Four Aedes aegypti Populations from Different Brazilian Regions

Role transformation of fecundity and viability: The leading cause of fitness costs associated with beta-cypermethrin resistance in Musca domestica

The need to harmonize insecticide resistance testing: methodology, intensity concentrations and molecular mechanisms evaluated inAedes aegyptipopulations in Central America and Hispaniola

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