Assessing the drug release from nanoparticles: Overcoming the shortcomings of dialysis by using novel optical techniques and a mathematical model

Xie, Li; Beyer, Susanne; Vogel, Vitali; Wacker, Matthias G.; Mäntele, Werner

doi:10.1016/j.ijpharm.2015.03.080

Cited by 50 publications

(32 citation statements)

References 53 publications

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“…The quantity of drug detected in the receiving compartment is generally considered as the amount of drug released from formulations. However, experimental results of release quantification methods utilizing dialysis membrane can be misleading since donor compartment does not satisfy the sink condition in majority of cases 2–4 . In addition, dialysis method has some potential limitations and several considerations should be taken into account in drug quantitation as summarized below.…”

Section: Introductionmentioning

confidence: 99%

“…(e) Dialysis is a two-step process: diffusion of drug from the carrier to the interior of the dialysis membrane and then the external reservoir. The drug release is governed by the partition between two phases (donor and receiving compartments) separated by the dialysis membrane 2–4 (Figure 1B). (f) Dialysis bag with a higher drug concentration requires a longer-time for the drug concentration equilibrium between compartments.…”

Section: Introductionmentioning

confidence: 99%

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Real-Time Analysis of Tenofovir Release Kinetics Using Quantitative Phosphorus (31P) Nuclear Magnetic Resonance Spectroscopy

Agrahari

Meng

Purohit

et al. 2017

Journal of Pharmaceutical Sciences

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The dialysis method is classically used for drug separation before analysis, but, does not provide direct and real-time drug quantification and has limitations affecting the dialysis rate. In this study, a phosphorus nuclear magnetic resonance (31P-qNMR) method is developed for the real-time quantification of therapeutic molecules in vitro. The release kinetics of model drug tenofovir (TFV: anti-HIV microbicide) was analyzed in vaginal fluid simulant (VFS), semen fluid simulant (SFS), and human plasma (HP) from chitosan nanofibers (size ~100–200nm) using the NMR (direct) method and compared with dialysis/UV-Vis (indirect) method. The assay was linear in VFS/SFS (0.20–5.0mM), HP (0.30–5.0mM) and specific (no drug 31P-qNMR chemical shift (~15ppm) interference with formulation/media components). LOD values were 0.075/0.10/0.20mM, whereas, LOQ values were 0.20/0.20/0.30mM in VFS/SFS/HP, respectively. The method was robust, precise (%RSE <2%), and accurate (%mean recovery: 90-110%). After 12h, ~75%/72%/70%w/w of TFV release was observed with 31P-qNMR, compared to ~47%/52%/52%w/w by dialysis method in VFS/SFS/HP, respectively. Approximately 20% decrease in %drug release observed with dialysis method suggested an interference with drug transport process due to the dialysis membrane and the Gibbs-Donnan effect. Overall, 31P-qNMR provides more accurate, real-time and direct drug quantification for effective in vitro/in vivo correlation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Real-Time Analysis of Tenofovir Release Kinetics Using Quantitative Phosphorus (31P) Nuclear Magnetic Resonance Spectroscopy

Agrahari

Meng

Purohit

et al. 2017

Journal of Pharmaceutical Sciences

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“…To overcome the reported shortcomings of dialysis assays for assessing drug release by nanoparticles, 26 parallel experiments were performed under the above conditions on a drug solution as a reference; this allowed the adsorption of vancomycin on the dialysis membrane to be explored and the fraction available for passing from the donor to the acceptor compartment (f 4 C ) to be estimated as follows:…”

Section: Size and Zeta Potentialmentioning

confidence: 99%

Development and In Vitro Evaluation of a Novel Drug Delivery System (Albumin Microspheres Containing Liposomes) Applied to Vancomycin

Valle

Salicio

Navarro

2016

Journal of Pharmaceutical Sciences

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“…Although current methods can be applied to measure the response of NPs to external stimuli under ideal conditions in laboratory set-ups, they cannot be used in less ideal environments such as in complex biological fluids, where it would be important to grasp the NPs specific evolution and modifications (Xie 2015, Itoh 2015, Mukherjee 2008, Huang 2013. In this paper, we will present a novel technique for the characterization of NPs in solution.…”

Section: Introductionmentioning

confidence: 99%

Confocal Depolarized Dynamic Light Scattering: a Novel Technique for the Characterization of Nanoparticles in Complex Fluids

Potenza

Sanvito

Mariani

et al. 2015

MRAJ

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We present Confocal Depolarized Dynamic Light Scattering as a novel optical method for the characterization of nanoparticles in solution. The method is validated in conditions of biological interest where traditional optical methods fail. As a test case we study highly concentrated, strongly scattering, samples of thermosensitive core-shell particles constituted by a spherical PMMA core surrounded by a PNIPAM network and, follow the kinetics of the processes induced by temperature changes. We prove that through the confocal optical scheme, the multiple scattering contribution is reduced by orders of magnitude.Moreover, the method allows the efficient characterization of NPs with a superior accuracy in particle size determination. Low degrees of polydispersity can be easily characterized through an adapted cumulant method.

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Assessing the drug release from nanoparticles: Overcoming the shortcomings of dialysis by using novel optical techniques and a mathematical model

Cited by 50 publications

References 53 publications

Real-Time Analysis of Tenofovir Release Kinetics Using Quantitative Phosphorus (31P) Nuclear Magnetic Resonance Spectroscopy

Real-Time Analysis of Tenofovir Release Kinetics Using Quantitative Phosphorus (31P) Nuclear Magnetic Resonance Spectroscopy

Development and In Vitro Evaluation of a Novel Drug Delivery System (Albumin Microspheres Containing Liposomes) Applied to Vancomycin

Confocal Depolarized Dynamic Light Scattering: a Novel Technique for the Characterization of Nanoparticles in Complex Fluids

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