Assessing the challenges of Fourier transform infrared spectroscopic analysis of blood serum

Abstract

There are many approaches to measuring the infrared spectrum of a blood serum sample. Naturally, each approach will have both advantages and disadvantages. We report on the progress of the application of infrared spectroscopy in the field of blood serum analysis towards clinical application, with a focus on prostate cancer. In order to perform a high‐powered study with clinical relevance, choosing the most suitable approach must undergo careful consideration. We review the possibilities of using different sample preparation methods and speculate upon the potential pitfalls of both transmission and attenuated total reflectance (ATR) techniques. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

Vibrational spectroscopic analysis of body fluids: avoiding molecular contamination using centrifugal filtration

“…Therefore, the use of centrifugal devices can greatly improve the sensitivity of the analysis by separating the different molecules according to their molecular weight 24,39,40 . Figure 2 shows the spectrum of the dried complete serum sample (A), the >100K serum fraction (B), and the <100Kserum fraction (D).…”

“…The spectra of the filtrates were taken from samples which were processed using unwashed centrifugal filter devices. [34][35][36][37][38][39][40] .…”

“…The data presented in figure 5C clearly highlight that, as seen in figure 4D, no traces of glycerine ( Figure 3) or NaOH ( Figure 5A) can be seen. of centrifugal devices is a promising approach for the field of vibrational spectroscopy applied to body fluids based diagnosis, the relevancy of preliminary studies that can be found in the literature is questionable due to the presence of molecular contamination of the samples by glycerine following ultra-filtration, obviously greatly reducing the accuracy of the analysis 24,39,40 . The true spectrum of the filtrate fraction is only achievable after appropriate decontamination.…”

A review of novel analytical diagnostics for liquid biopsies: spectroscopic and spectrometric serum profiling of primary and secondary brain tumors

“…Studies have reviewed the characteristics and advantages of the technique (Hughes et al. 2016); the proposed process is highlighted in Figure 2 (Baker et al. 2014).…”

“…Dorling and Baker, presented a schematic highlighting the potential for serum analysis using spectroscopy within a clinical environment, expanded further by Hughes et al. (2016) (Hughes and Baker 2016) – see Figure 3.…”