Assessing the bioremediation potential of indigenously isolated Klebsiella sp. WAH1 for diclofenac sodium: optimization, toxicity and metabolic pathway studies

Sharma, Saloni; Setia, Hema; Toor, Amrit Pal

doi:10.1007/s11274-021-02998-4

Cited by 13 publications

(5 citation statements)

References 56 publications

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“…Klebsiella sp. WAH1 has been reported to have a diclofenac degradation pathway highly developed reactions of hydroxylation, decarboxylation, and dechlorination (Sharma et al, 2021). Researchers are trying for more bioactive prednisolone derivatives with microbial interference.…”

Section: Resultsmentioning

confidence: 99%

Primary studies on biotransformation of steroidal drug Prednisolone

Waghmode¹,

Bankar²,

Gaikwad³

et al. 2022

Nep J Environ Sci

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The less biodegradable steroidal drug, Prednisolone is one of the widely used drugs in the treatment of autoimmune and infectious diseases. Transformation of prednisolone can be achieved with microbial activity. Isolation of prednisolone-resistant microorganisms was done using a sewage sample. Out of 5 isolates, one isolate was selected for further studies based on maximum tolerability to prednisolone. The isolate was identified based on MALDI-TOF. The strain was found to have a match score of 2.336 with gram-negative bacteria Klebsiella pneumoniae spp pneumoniae DSM3010 4T HAM. The isolate was found to degrade 80% prednisolone on the 5th day of fermentation. The concentration of prednisolone was determined using a colorimetric-based method and HPLC technique.

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Section: Resultsmentioning

confidence: 99%

Primary studies on biotransformation of steroidal drug Prednisolone

Waghmode¹,

Bankar²,

Gaikwad³

et al. 2022

Nep J Environ Sci

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“…Each bacterial isolate was tested for its ability to grow at different concentrations of DCF (10,20,30, and 40 mg/L) as the sole carbon source, and isolates were enumerated by viable counts using Luria Bertani agar medium (Laboratories Conda SA, Madrid, Spain) at pH 7.0 after aerobic incubation at 37 • C for 24 h. Bacterial growth was confirmed by Gram staining; the experiment was repeated three times in the same conditions, and the viable count was performed in all samples in triplicate. The counted bacterial colonies were expressed as log 10 CFU/mL and plotted against the time of incubation [16].…”

Section: Optimization Of Bacterial Isolate Growth Under Different Phy...mentioning

confidence: 99%

Complete Biodegradation of Diclofenac by New Bacterial Strains: Postulated Pathways and Degrading Enzymes

Mohamed,

Asair,

Fetyan

et al. 2023

Microorganisms

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The accumulation of xenobiotic compounds in different environments interrupts the natural ecosystem and induces high toxicity in non-target organisms. Diclofenac is one of the commonly used pharmaceutical drugs that persist in the environment due to its low natural degradation rate and high toxicity. Therefore, this study aimed to isolate potential diclofenac-degrading bacteria, detect the intermediate metabolites formed, and determine the enzyme involved in the degradation process. Four bacterial isolates were selected based on their ability to utilize a high concentration of diclofenac (40 mg/L) as the sole carbon source. The growth conditions for diclofenac degradation were optimized, and bacteria were identified as Pseudomonas aeruginosa (S1), Alcaligenes aquatilis (S2), Achromobacter spanius (S11), and Achromobacter piechaudii (S18). The highest percentage of degradation was recorded (97.79 ± 0.84) after six days of incubation for A. spanius S11, as analyzed by HPLC. To detect and identify biodegradation metabolites, the GC-MS technique was conducted for the most efficient bacterial strains. In all tested isolates, the initial hydroxylation of diclofenac was detected. The cleavage step of the NH bridge between the aromatic rings and the subsequent cleavage of the ring adjacent to or in between the two hydroxyl groups of polyhydroxylated derivatives might be a key step that enables the complete biodegradation of diclofenac by A. piechaudii S18, as well as P. aeruginosa S1. Additionally, the laccase, peroxidase, and dioxygenase enzyme activities of the two Achromobacter strains, as well as P. aeruginosa S1, were tested in the presence and absence of diclofenac. The obtained results from this work are expected to be a useful reference for the development of effective detoxification bioprocesses utilizing bacterial cells as biocatalysts. The complete removal of pharmaceuticals from polluted water will stimulate water reuse, meeting the growing worldwide demand for clean and safe freshwater.

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“…93 Different microbial remediation studies on the removal efficiency of pharmaceutical pollutants are listed in Table 3. 95–119…”

Section: Microbial Degradationmentioning

confidence: 99%

Promising approaches and kinetic prospects of the microbial degradation of pharmaceutical contaminants

Karishma,

Yaashikaa,

Kumar

et al. 2023

Environ. Sci.: Adv.

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Assessing the bioremediation potential of indigenously isolated Klebsiella sp. WAH1 for diclofenac sodium: optimization, toxicity and metabolic pathway studies

Cited by 13 publications

References 56 publications

Primary studies on biotransformation of steroidal drug Prednisolone

Primary studies on biotransformation of steroidal drug Prednisolone

Complete Biodegradation of Diclofenac by New Bacterial Strains: Postulated Pathways and Degrading Enzymes

Promising approaches and kinetic prospects of the microbial degradation of pharmaceutical contaminants

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