Assessing the bioavailability of the Novel Met-Met product (AQUAVI® Met-Met) compared to dl-methionine (dl-Met) in white shrimp (Litopenaeus vannamei)

Niu, Jin; Lemme, A.; He, Jia; Li, Haoyue; Xie, Shiwei; Liu, Yongjian; Yang, Hui‐Jun; Figueiredo-Silva, Cláudia; Liu, Tian

doi:10.1016/j.aquaculture.2017.08.021

Cited by 30 publications

(44 citation statements)

References 39 publications

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“…Pea protein isolate has favourable amino acid profile; thus, less IAA were added to the PPI diets. Some studies demonstrated that IAA have similar bioavailability to the protein‐bound amino acids (Hu et al, ; Zarate, Lovell, & Payne, ), whereas other studies found that IAA have higher absorption rate than protein‐bound amino acids (Niu et al, ; Xie et al, ). The different absorption rates of IAA and protein‐bounded amino acids may affect the oxidation and deposition of other amino acids.…”

Section: Discussionmentioning

confidence: 99%

Partial replacement of fishmeal with corn gluten meal, pea protein isolate and their mixture in diet of black sea bream ( Acanthopagrus schlegelii ) juveniles: Effects on growth performance, feed utilization and haematological parameters

et al. 2020

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Corn gluten meal (CGM), pea protein isolate (PPI) and their mixture (CPP, 1:1 ratio) were evaluated as fishmeal (FM) alternatives in black sea bream (Acanthopagrus schlegelii) juveniles (9.02 ± 0.12 g). A FM diet was designed as control, and other six diets had 20% and 40% FM protein replaced by CGM, PPI and CPP, respectively, with the supplementation of crystalline methionine, arginine and lysine. After the 8‐week feeding trial, significantly lower weight gain was found in fish fed the 40% CGM diet (p < .05), whereas other treatments had no statistical difference (p > .05). The values of feed efficiency ratio and feed intake, as well as proximate compositions of muscle and whole body, were not significantly influenced in all treatments. Apparent digestibility coefficients (ADCs) of dry matter, crude protein, threonine, valine, methionine, isoleucine, leucine, phenylalanine and lysine were significantly influenced by protein source. Fish fed the 40% CGM diet had significantly higher content of essential amino acid in muscle than that of the 20% PPI diet. Fish fed the 40% CGM diet had significantly lower content of serum cholesterol than other treatments (p < .05). Elevated serum superoxide dismutase activity was found in fish fed the 40% plant protein diets. In conclusion, between 20% and 40% FM protein could be replaced by CGM, while about 40% FM protein derived from PPI and CPP could be applied in black sea bream.

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Section: Discussionmentioning

confidence: 99%

Partial replacement of fishmeal with corn gluten meal, pea protein isolate and their mixture in diet of black sea bream ( Acanthopagrus schlegelii ) juveniles: Effects on growth performance, feed utilization and haematological parameters

et al. 2020

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“…Some studies have demonstrated that crystalline amino acids and protein‐bound amino acids had similar bio‐efficiency value (Hu et al, ; Zarate, Lovell, & Payne, ). Furthermore, Met‐Met has a higher absorption rate than protein‐bound methionine and has a higher bioavailability value than dl ‐Met (Niu et al, ; Xie et al, ), so the supplementation of Met‐Met in plant‐protein based diets could effectively improve the growth performance of shrimp. Highest SGR and WG were obtained in shrimp fed the PC diet, which was significantly higher than shrimp fed the MM 0.3 diet.…”

Section: Discussionmentioning

confidence: 99%

“…A novel dipeptide dl ‐methionyl‐ dl ‐methionine (“Met‐Met”, branded as AQUAVI ® Met‐Met) has special physical and chemical characteristics compared with other free‐form crystalline methionine sources selected by Evonik's Aqua R&D Group. AQUAVI ® Met‐Met is the mixture of four different methionine stereoisomers ( dl ‐Met‐Met, ld ‐Met‐Met, dd ‐Met‐Met and ll ‐Met‐Met) which could be efficiently cleaved by digestive enzymes to free d ‐ and l ‐methionine, and the higher bioavailability value than dl ‐Met has already been demonstrated in white shrimp (Façanha, Oliveira‐Neto, Figueiredo‐Silva, & Nunes, ; Niu et al, ; Xie et al, ) . As shrimp are benthos feeders with slow feeding behaviour, part of supplemented methionine could be lost by leaching before ingestion (Gu et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effects of dietarydl‐methionyl‐dl‐methionine (Met‐Met) on growth performance, body composition and haematological parameters of white shrimp (Litopenaeus vannamei) fed with plant protein–based diets

et al. 2019

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A 10‐week feeding trial was conducted to evaluate the effects of supplementing different levels of dl‐methionyl‐dl‐methionine (AQUAVI® Met‐Met) in plant protein–based diets on Litopenaeus vannamei. The positive control (PC) and negative control (NC) diets were designed with 20% and 8% fishmeal respectively, and other six diets were formulated with graded levels of Met‐Met from 0.05% to 0.30% with a 0.05% increment on the basis of NC diet (MM 0.05–MM 0.3). Six replicates were randomly assigned to each diet with 50 shrimp each having initial weight of 0.98 ± 0.02 g. The variation of FM concentration from 20% to 8% and supplemented with graded levels of Met‐Met did not affect the survival rate, feed conversion ratio, protein efficiency ratio, whole body and muscle proximate compositions (p > 0.05). However, diets with ≤0.20% Met‐Met supplementation resulted in significantly increased weight gain and specific growth rate, after which both parameters reached plateau. Shrimp fed the NC diet showed significantly lower total essential amino acid (EAA) content in muscle (p < 0.05). Supplementation of Met‐Met significantly improved apparent digestibility coefficients of dry matter, crude protein, lipid, phosphorus and EAAs (p < 0.05). Based on broken‐line analysis, the methionine requirement for white shrimp was estimated to be 0.87% when using Met‐Met as methionine source.

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“…This was not reflected in feed intake data in our study. While dl ‐Methionyl‐ dl ‐Methionine is known to have less leaching in water and a high bioefficacy in whiteleg shrimp diets (Façanha et al, , ; Niu et al, ; J.‐J. Xie et al, ), other sources of feed‐grade CAAs are likely prone to leaching in water.…”

Section: Discussionmentioning

confidence: 99%

“…This was not reflected in feed intake data in our study. While DL-Methionyl-DL-Methionine is known to have less leaching in water and a high bioefficacy in whiteleg shrimp diets (Façanha et al, 2016(Façanha et al, , 2018Niu et al, 2018;, other sources of feed-grade CAAs are likely prone to leaching in water. Pellet stability of the experimental diets was measured by examining Met levels of the diets subjected to a leaching test.…”

Section: Discussionmentioning

confidence: 99%

Crude protein in low‐fish meal diets for juvenile Litopenaeus vannamei can be reduced through a well‐balanced supplementation of essential amino acids

Nunes

Sabry‐Neto

Masagounder

2019

J World Aquaculture Soc

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The use of supplemental essential amino acids (EAAs) has been shown to provide an opportunity to minimize excess levels of crude protein (CP) in animal feeds. The present study investigated the effect of reducing the amount of CP in low‐fish meal diets (5%) for juvenile Litopenaeus vannamei. Four sets of diets were prepared containing (% on a fed basis, mean ± SD) 31.24 ± 0.71, 33.70 ± 0.41, 36.90 ± 0.44, and 39.63 ± 0.14% CP, with each protein level containing a total dietary methionine (Met) (Met + Cys) level of 0.56 ± 0.02 (1.07 ± 0.23), 0.71 ± 0.01 (1.22 ± 0.20), 0.88 ± 0.02 (1.38 ± 0.22), or 1.04 ± 0.02% (1.55 ± 0.18%). Shrimp of 1.00 ± 0.08 g were stocked in 84 outdoor tanks of 1 m3 at a rate of 100 shrimp/m2 and raised for 75 days. Final survival ranged from 83 to 97% and was unaffected by Met content. Both survival and yield were significantly depressed when shrimp were fed the 31% CP diet. Shrimp grew at a weekly rate of between 0.79 and 0.97 g, achieving a final body weight (BW) in excess of 10.8 g. There was a significant interaction between CP and Met over BW. Shrimp fed 0.56% Met achieved the lowest BW at harvest. Increasing CP beyond 34% did not enhance BW. With a dietary Met content of 0.71%, the highest BW was achieved with 34% CP compared to other levels. There was a significant improvement in Feed Conversion Ratio (FCR) when CP was raised from 31 to 34%. Similarly, dietary Met levels above 0.71% resulted in a significantly better FCR compared to 0.56%. Our study has shown that, if dietary Met (Met + Cys) meets a minimum of 0.71% (1.22%), levels of CP could be reduced from 40 to 34% without adverse effects on shrimp performance.

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Assessing the bioavailability of the Novel Met-Met product (AQUAVI® Met-Met) compared to dl-methionine (dl-Met) in white shrimp (Litopenaeus vannamei)

Cited by 30 publications

References 39 publications

Partial replacement of fishmeal with corn gluten meal, pea protein isolate and their mixture in diet of black sea bream ( Acanthopagrus schlegelii ) juveniles: Effects on growth performance, feed utilization and haematological parameters

Partial replacement of fishmeal with corn gluten meal, pea protein isolate and their mixture in diet of black sea bream ( Acanthopagrus schlegelii ) juveniles: Effects on growth performance, feed utilization and haematological parameters

Effects of dietarydl‐methionyl‐dl‐methionine (Met‐Met) on growth performance, body composition and haematological parameters of white shrimp (Litopenaeus vannamei) fed with plant protein–based diets

Crude protein in low‐fish meal diets for juvenile Litopenaeus vannamei can be reduced through a well‐balanced supplementation of essential amino acids

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