Assessing the Anti-inflammatory Mechanism of Reduning Injection by Network Pharmacology

Abstract

Reduning Injection (RDNI) is a traditional Chinese medicine formula indicated for the treatment of inflammatory diseases. However, the molecular mechanism of RDNI is unclear. The information of RDNI ingredients was collected from previous studies. Targets of them were obtained by data mining and molecular docking. The information of targets and related pathways was collected in UniProt and KEGG. Networks were constructed and analyzed by Cytoscape to identify key compounds, targets, and pathways. Data mining and molecular docking identified 11 compounds, 84 targets, and 201 pathways that are related to the anti-inflammatory activity of RDNI. Network analysis identified two key compounds (caffeic acid and ferulic acid), five key targets (Bcl-2, eNOS, PTGS2, PPARA, and MMPs), and four key pathways (estrogen signaling pathway, PI3K-AKT signaling pathway, cGMP-PKG signaling pathway, and calcium signaling pathway) which would play critical roles in the treatment of inflammatory diseases by RDNI. The cross-talks among pathways provided a deeper understanding of anti-inflammatory effect of RDNI. RDNI is capable of regulating multiple biological processes and treating inflammation at a systems level. Network pharmacology is a practical approach to explore the therapeutic mechanism of TCM for complex disease.

Clinical Observation of Reduning Combined with Recombinant Human Interferon α-2b in the Treatment of Children with Viral Pneumonia

“…Gardenia can conduct toxin down, eliminate the fire of Sanjiao, and enhance the detoxification effect of honeysuckle, and modern pharmacological research believes that it has anti-inflammatory, antiviral, cooling and antipyretic effects. Artemisia can relieve exterior syndrome, clear away the heat, and the main component of Artemisia annua has anti-inflammatory, antipyretic, analgesic, and immunity-enhancing effects, and artemisinin and artemether have the function of promoting the proliferation of spleen T lymphocytes, and exert direct effect in inactivating and inhibiting proliferation of influenza virus [ 21 , 22 ].…”

A Five-Dimensional Network Meta-Analysis of Chinese Herbal Injections for Treating Acute Tonsillitis Combined With Western Medicine

“…The antipyretic mechanism of RDN might be related to the regulation of biosynthesis as well as sphingolipid metabolism of valine, leucine, and isoleucine (Gao et al, 2020). In addition, RDN was reported to possess anti-inflammatory and antiviral effects (Cao et al, 2015;Xie et al, 2020;Xu et al, 2021), which might work in treating acute tonsillitis. SHL is made from active ingredients of Forsythia suspensa (Thunb.)…”