Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells

Abstract

Background & objectives:Adherence of bacteria to epithelial cells and mucosal surfaces is a key criterion for selection of probiotic. We assessed the adhesion property of selected indigenous probiotic Lactobacillus strains based on their hydrophobicity and ability to adhere to human epithelial cells.Methods:Five human faecal Lactobacillus isolates, one from buffalo milk and one from cheese were assessed for hydrophobicity following the microbial adhesion to hydrocarbons (MATH) method and colonization potentials based on their adherence to Caco2 and HT-29 colonic adenocarcinomal human intestinal epithelial cell lines. Lactobacillus strains that adhered to Caco2 and HT-29 cell lines were quantified by plating after trypsinization and simultaneously the adhered bacteria were also examined microscopically after staining with Geimsa stain and counted in different fields.Results:Among the tested faecal isolates, L. plantarum Lp91 showed maximum percentage hydrophobicity (35.73±0.40 for n-hexadecane and 34.26±0.63 for toluene) closely followed by L. plantarum Lp9 (35.53±0.29 for n-hexadecane and 33.00±0.57 for toluene). Based on direct adhesion to epithelial cells, L. plantarum Lp91 was the most adhesive strain to HT-29 and Caco2 cell lines with per cent adhesion values of 12.8 ± 1.56 and 10.2 ± 1.09, respectively. L. delbrukeii CH4, was the least adhesive with corresponding figures of 2.5 ± 0.37 and 2.6 ± 0.20 per cent on HT-29 and Caco2 cell lines. Adhesion of the six isolated Lactobacillus strain to HT-29 cell and Caco2 lines as recorded under microscope varied between 131.0 ± 13.9 (Lp75) to 342.7 ± 50.52 (Lp91) and 44.7 ± 9.29 (CH4) to 315.7± 35.4 (Lp91), respectively.Interpretation & conclusions:Two Indigenous probiotic Lactobacillus strains (Lp9, Lp91) demonstrated their ability to adhere to epithelial cell and exhibited strong hydrophobicity under in vitro conditions, and thus could have better prospects to colonize the gut with extended transit

Assessment of Resistance and Bioremediation Ability ofLactobacillusStrains to Lead and Cadmium

“…The results indicated that most microorganisms studied were relatively hydrophilic (Table 1). The hydrophilic nature of lactobacilli has often been encountered in previous studies [15, 18, 19]. Three strains, L. plantarum B-578 (52.0 ± 6.4%), L. brevis 20054 (63.1 ± 5.6%), and L. buchneri 20057 (66.9 ± 6.3%), were hydrophobic.…”

In vitro assessment of Pediococcus acidilactici Kp10 for its potential use in the food industry

“…Colonization with extended transit time is most critical for optimal expression of general and specific physiological functions of probiotic microorganisms. Probiotic strains invariably should demonstrate the ability to adhere to the surface mucosal epithelial cells, an important requirement with reference to effective colonization [24]. Cell adhesion which involve contact between the cell membrane of the bacteria and that of the mucosal epithelium is no doubt a complex process.…”

Antagonistic Activity of Lactobacillus reuteri Strains on the Adhesion Characteristics of Selected Pathogens

“…Therefore, adhesion ability can be considered as a standard biomarker for selecting a potential probiotic (Duary et al, 2011). In the present investigation, we evaluated eight probiotic strains of L. reuteri , previously isolated from breast fed infant feces (Singh et al, 2012), for their potential to adhere Caco-2 cells.…”

“…Following incubation, the Caco-2 monolayers were washed with sterile PBS (pH 7.4), Giemsa-stained and examined microscopically under oil immersion, as described previously by Duary et al (2011).…”

“…There are numerous probiotic genera and species including lactobacilli and bifidobacteria which have been implicated in a number of health promoting functions that affect general health and well-being of the host. Adhesion is considered as a potential biomarker for selection of potential probiotics; as their colonization with extended transit time is extremely crucial for optimal expression of their general as well as specific physiological functions (Duary et al, 2011). Several reports have given special attention to the protective role of probiotics against enteropathogens and the underlying mechanisms (Salminen et al, 1998, 1999; Ouwehand et al, 2002; Ouwehand and Salminen, 2003; Collado et al, 2007).…”