Assessing temporal flux of plant hormones in stored processing potatoes using high definition accurate mass spectrometry

Ordaz-Ortíz, José Juan; Foukaraki, Sofia G.; Terry, Leon A.

doi:10.1038/hortres.2015.2

Cited by 30 publications

(21 citation statements)

References 26 publications

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“…The final dried material was reconstituted with 400 μL of 0.1% formic acid in ultrapure water. To quantify the five plant hormones, 5 μL of the reconstituted sample was injected into an Agilent 6540 Ultra High Definition Accurate Mass quadrupole time-of-flight liquid chromatography mass spectrometry (Q-TOF LC-MS) system using a Dual ESI Agilent Jet Stream source in a negative and positive mode according to Ordaz-Ortiz et al 27 A ZORBAX Eclipse Plus C18 column, 2.1 mm × 50 mm diameter and 1.8 μm particle size (Agilent, part no. 959757-902) coupled with a 2.1 mm × 5 mm diameter and 1.8 μm ZORBAX Eclipse Plus C18 guard column (Agilent, part no.…”

Section: Extraction and Quantification Of Plant Hormonesmentioning

confidence: 99%

Investigating the involvement of ABA, ABA catabolites and cytokinins in the susceptibility of ‘Nules Clementine’ mandarin to rind breakdown disorder

et al. 2019

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BACKGROUND ‘Nules Clementine’ mandarin was used to investigate the potential involvement of endogenous plant hormones in mediating citrus fruit susceptibility to rind breakdown disorder (RBD). The effect of light exposure (namely canopy position and bagging treatments) on the endogenous concentration of ABA, 7′‐hydroxy‐abscisic acid (7‐OH‐ABA), ABA‐glucose ester (ABA‐GE) and dihydrophaseic acid (DPA), and t‐zeatin was tested using four preharvest treatments: outside, outside bagged, inside and inside bagged. Phytohormones concentration was evaluated during nine weeks of postharvest storage at 8 °C. RESULTS The shaded fruit inside the canopy had the highest RBD score (0.88) at the end of postharvest storage, while sun‐exposed fruit had the lowest score (0.12). Before storage, ABA concentration was lowest (462.8 µg kg−1) for inside fruit, and highest in outside bagged fruit (680.5 µg kg−1). Although ABA concentration suddenly increased from the third week, reaching a maximum concentration of 580 µg kg−1 at week 6 in fruit from inside position, it generally reduced 1.6‐fold ranging from 240.52 to 480.65 µg kg−1 throughout storage. The increase of 7‐OH‐ABA was more prominent in fruit from inside canopy. Overall, the concentration of ABA‐GE increased three‐fold with storage time. DPA concentration of bagged fruit from inside canopy position was significantly higher compared to outside fruit. The lower ABA‐GE and higher DPA concentration in inside bagged fruit throughout storage also coincided with higher RBD. CONCLUSION The strong positive correlations between 7‐OH‐ABA, DPA and RBD incidence demonstrated that these ABA catabolites could be used as biomarkers for fruit susceptibility to the disorder. © 2019 Society of Chemical Industry

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Section: Extraction and Quantification Of Plant Hormonesmentioning

confidence: 99%

Investigating the involvement of ABA, ABA catabolites and cytokinins in the susceptibility of ‘Nules Clementine’ mandarin to rind breakdown disorder

et al. 2019

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“…ABA and its derivatives were extracted according to Ordaz-Ortiz et al (2015) with some modifications. One hundred and fifty mg of fresh ground tissue was extracted with 5 mL of methanol/water/formic acid (75:20:5 v/v/v).…”

Section: Methodsmentioning

confidence: 99%

“…The MS/MS detection was performed using the multiple reactions monitoring mode (MRM) at −4500 V, 25 psi nebulization pressure and 10 L min -1 nitrogen flow. Deuterated standards were purchased from Olchemim and Canada National Research Council of Canada-Plant Biotechnology Institute (Ordaz-Ortiz et al, 2015). ABA standard was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Expression of a glucomannan mannosyltransferase gene (GMMT) from Aloe vera is induced by water deficit and abscisic acid

Salinas

Aguilar-Salinas

Contreras³

et al. 2018

Preprint

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Highlight 1 GMMT (a possible CSLA9) from Aloe vera is upregulated during water stress. Aloe 2 vera GMMT expression is also induced by exogenous application of the plant 3 stress hormone abscisic acid (ABA) in non-water-stressed plants. 4 5 3 Summary 6 In Aloe barbadensis Miller (Aloe vera), a xerophytic crassulacean acid metabolism 7 (CAM) plant, the main polysaccharide of the gel present in the leaves is an 8 acetylated glucomannan named acemannan. This polysaccharide is responsible 9for the plant succulence, helping it to retain water. In this study we determined 10 using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) that the 11 acemannan is a glucomannan without galactose side branches. We also 12 investigated the expression of the gene responsible for acemannan backbone 13 synthesis, encoding a glucomannan mannosyltransferase (GMMT). It was found 14 by in silico analyses that the GMMT gene belongs to the cellulose synthase like A 15 type-9 (CSLA9) subfamily. Using RT-qPCR it was found that the expression of 16 GMMT increased in Aloe vera plants subjected to water stress. This expression 17 correlates with an increase of endogenous ABA levels, suggesting that the gene 18 expression could be regulated by ABA. To corroborate this hypothesis, exogenous 19 ABA was applied to non-water-stressed plants, increasing the expression of GMMT 20 significantly 48 h after ABA treatment. 21 22

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“…Similar declines in the content of ABA during storage has been reported in potato cv. Marfona (Ordaz-Ortiz et al, 2015;Foukaraki et al, 2016a) and in onion cvs. Red Baron, Sherpa and Wellington (Chope et al, 2006).…”

Section: Hormonal Changes and Sprout Growthmentioning

confidence: 99%

The timing of exogenous ethylene supplementation differentially affects stored sweetpotato roots

Amoah

Landahl

Terry

2016

Postharvest Biology and Technology

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The effects of continuous supplemental ethylene (10 μL L-1) timing on the physiology and biochemistry of sweetpotato roots during storage at 25 o C were examined. Alongside continuous ethylene or air treatments, a subset of the roots were transferred at dormancy break, from those previously stored in ethylene into air and vice-versa. The study showed distinctive ethylene-induced effects on the metabolism of individual sugars, phenolic compounds and phytohormones (abscisic acid and zeatin riboside) across the spatial gradient of the root flesh and skin tissues. Although ethylene fumigation doubled root respiration, sprout growth was significantly suppressed. Supplementation of roots with ethylene after dormancy break effectively inhibited sprout growth as much as continuous ethylene alone. On the other hand, truncating ethylene application after dormancy release promoted vigorous sprout growth. After prolonged storage, ethylene treatment was associated with increased weight loss and incidence of proximal rots. Supplemental ethylene also accelerated the catabolism of monosaccharides, and promoted accumulation of phenolic compounds in the proximal root sections.

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Assessing temporal flux of plant hormones in stored processing potatoes using high definition accurate mass spectrometry

Cited by 30 publications

References 26 publications

Investigating the involvement of ABA, ABA catabolites and cytokinins in the susceptibility of ‘Nules Clementine’ mandarin to rind breakdown disorder

Investigating the involvement of ABA, ABA catabolites and cytokinins in the susceptibility of ‘Nules Clementine’ mandarin to rind breakdown disorder

Expression of a glucomannan mannosyltransferase gene (GMMT) from Aloe vera is induced by water deficit and abscisic acid

The timing of exogenous ethylene supplementation differentially affects stored sweetpotato roots

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