Assessing Surface Adsorption in Cyclic Olefin Copolymer Microfluidic Devices Using Two-Dimensional Nano Liquid Chromatography-Micro Free Flow Electrophoresis Separations
Cecilia C. Douma,
Michael T. Bowser
Abstract:Surface interactions
are a concern in microscale separations, where
analyte adsorption can decrease the speed, sensitivity, and resolution
otherwise achieved by miniaturization. Here, we functionally characterize
the surface adsorption of hot-embossed cyclic olefin copolymer (COC)
micro free-flow electrophoresis (μFFE) devices using two-dimensional
nLC × μFFE separations, which introduce a 3- to 5 s plug
of analyte into the device and measure temporal broadening that arises
from surface interactions. COC is an… Show more
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