“…Over the past few years, several studies have demonstrated the feasibility of metabarcoding-based monitoring of freshwater macroinvertebrates (Andújar et al, 2018;Carew, Pettigrove, Metzeling, & Hoffmann, 2013;Gibson et al, 2015;Hajibabaei, Shokralla, Zhou, Singer, & Baird, 2011). Despite some methodological limitations (presence/absence data, primer bias) (Piñol, Mir, Gomez-Polo, & Agustí, 2014;Elbrecht & Leese, 2015), assessment results are at least comparable if not superior to conventional morphology-based stream monitoring approaches (Elbrecht, Vamos, Meissner, Aroviita, & Leese, 2017;Emilson et al, 2018;Gibson et al, 2015). Some macroinvertebrate reference databases are already fairly comprehensive especially for common taxa (Carew et al, 2017;Curry, Gibson, Shokralla, Hajibabaei, & Baird, 2018).…”