Assessing size-dependent cytotoxicity of boron nitride nanotubes using a novel cardiomyocyte AFM assay

Augustine, Jerry; Cheung, Timothy; Gies, Valerie; Boughton, Jennifer; Chen, Maohui; Jakubek, Zygmunt J.; Walker, Scott A.; Martinez‐Rubi, Yadienka; Simard, Benoît; Zou, Shan

doi:10.1039/c9na00104b

Cited by 26 publications

(29 citation statements)

References 40 publications

(89 reference statements)

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“…[138] A recent study used AFM based cytotoxicity technique and showed that long BNNTs (>500 nm) are more cytotoxic than shortened BNNTs. [139] The latest BNNT report (June 2020) from the National Institute for Occupational Safety and Health (NIOSH) is a BNNT study in vitro and noted that higher doses of BNNT in pulmonary exposure can cause damage. [140] This biocompatibility is still being extensively studied and has not been conclusive due to the varying models.…”

Section: Toxicitymentioning

confidence: 99%

Structure, Properties and Applications of Two‐Dimensional Hexagonal Boron Nitride

et al. 2021

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Hexagonal boron nitride (h-BN) has emerged as a strong candidate for twodimensional (2D) material owing to its exciting optoelectrical properties combined with mechanical robustness, thermal stability, and chemical inertness. Super-thin h-BN layers have gained significant attention from the scientific community for many applications, including nanoelectronics, photonics, biomedical, anti-corrosion, and catalysis, among others. This review provides a systematic elaboration of the structural, electrical, mechanical, optical, and thermal properties of h-BN followed by a comprehensive account of stateof-the-art synthesis strategies for 2D h-BN, including chemical exfoliation, chemical, and physical vapor deposition, and other methods that have been successfully developed in recent years. It further elaborates a wide variety of processing routes developed for doping, substitution, functionalization, and combination with other materials to form heterostructures. Based on the extraordinary properties and thermal-mechanical-chemical stability of 2D h-BN, various potential applications of these structures are described.The ORCID identification number(s) for the author(s) of this article can be found under

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Section: Toxicitymentioning

confidence: 99%

Structure, Properties and Applications of Two‐Dimensional Hexagonal Boron Nitride

et al. 2021

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“…While this piezoceramic has been found to promote the proliferation, attachment, and osteogenic differentiation of MSCs, several studies have also reported their negative influence over chondrocytes, fibroblasts, and smooth muscle cells [ 42 , 48 ]. Contradictory data have been published regarding the toxicity and cytotoxicity of BNNTs, with the potential adverse health effects of this bioceramic restricting their current applications in TE [ 65 ].…”

Section: Piezoelectric Materialsmentioning

confidence: 99%

Piezoelectric Electrospun Fibrous Scaffolds for Bone, Articular Cartilage and Osteochondral Tissue Engineering

Barbosa

Ferreira

Silva

2022

IJMS

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Osteochondral tissue (OCT) related diseases, particularly osteoarthritis, number among the most prevalent in the adult population worldwide. However, no satisfactory clinical treatments have been developed to date to resolve this unmet medical issue. Osteochondral tissue engineering (OCTE) strategies involving the fabrication of OCT-mimicking scaffold structures capable of replacing damaged tissue and promoting its regeneration are currently under development. While the piezoelectric properties of the OCT have been extensively reported in different studies, they keep being neglected in the design of novel OCT scaffolds, which focus primarily on the tissue’s structural and mechanical properties. Given the promising potential of piezoelectric electrospun scaffolds capable of both recapitulating the piezoelectric nature of the tissue’s fibrous ECM and of providing a platform for electrical and mechanical stimulation to promote the regeneration of damaged OCT, the present review aims to examine the current state of the art of these electroactive smart scaffolds in OCTE strategies. A summary of the piezoelectric properties of the different regions of the OCT and an overview of the main piezoelectric biomaterials applied in OCTE applications are presented. Some recent examples of piezoelectric electrospun scaffolds developed for potentially replacing damaged OCT as well as for the bone or articular cartilage segments of this interfacial tissue are summarized. Finally, the current challenges and future perspectives concerning the use of piezoelectric electrospun scaffolds in OCT regeneration are discussed.

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“…The most dominant cell types used in the included articles were human embryonic kidney cells (HEK293, HEK293T) [24,25,74,81,154], human neuroblastoma cells (SH-SY5Y) [30,64,68,73,76,79,82,155], mouse embryonic fibroblasts (NIH/3T3) [70], human dermal fibroblasts (HDF) [31,36,48,51,55], lung fibroblasts (MRC-5) [65,156], dental pulp fibroblasts [34,41,62], lung epithelium cells (A549) [37,55,74], macrophage cells (RAW 264.7) [49,74], human liver epithelium (HeP G2) [27,28,37], human peripheral blood (NB4) [37], glioblastoma cells (malignant U87 [30,37,156], T98 [80,156]), mammary gland adenocarcinoma cells (MCF-7) [156], N9 murine microglia cells [50], osteoblasts [54,66,75,77], osteoblast precursor cell line derived from mouse musculus calvaria (M3CT3) [47]<...…”

Section: Cell Sourcesmentioning

confidence: 99%

“…The study highlighted the size and aspect ratio and absence of biomolecules that lead to higher toxicity levels in cell cultures [74]. Augustine et al [37] investigated a novel atomic force microscopy (AFM)-based cardiomyocyte assay that reliably assesses the cytotoxicity of BNNTs. High-energy probe sonication was used to modify and control the length of BNNTs [37].…”

Section: In Vitro Studies Stated Bnnts Are Cytotoxicmentioning

confidence: 99%

“…Augustine et al [37] investigated a novel atomic force microscopy (AFM)-based cardiomyocyte assay that reliably assesses the cytotoxicity of BNNTs. High-energy probe sonication was used to modify and control the length of BNNTs [37]. Cytotoxicity studies using the novel cardiomyocyte AFM model agreed with traditional colorimetric cell metabolic assays, both revealing a correlation between tube length and cytotoxicity with longer tubes having higher cytotoxicity [37].…”

Section: In Vitro Studies Stated Bnnts Are Cytotoxicmentioning

confidence: 99%

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In Vitro and In Vivo Cytotoxicity of Boron Nitride Nanotubes: A Systematic Review

Kakarla

Kong

2022

Nanomaterials

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Boron nitride nanotubes (BNNTs) are an exciting class of nanomaterials due to their unique chemical and physical characteristics. In recent decades, BNNTs have gained huge attention in research and development for various applications, including as nano-fillers for composites, semiconductor devices, hydrogen storage, and as an emerging material in biomedical and tissue engineering applications. However, the toxicity of BNNTs is not clear, and the biocompatibility is not proven yet. In this review, the role of BNNTs in biocompatibility studies is assessed in terms of their characteristics: cell viability, proliferation, therapeutic outcomes, and genotoxicity, which are vital elements for their prospective use in biomedical applications. A systematic review was conducted utilising the databases Scopus and Web of Science (WOS) (2008–2022). Additional findings were discovered manually by snowballing the reference lists of appropriate reviews. Only English-language articles were included. Finally, the significant analysis and discussion of the chosen articles are presented.

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Assessing size-dependent cytotoxicity of boron nitride nanotubes using a novel cardiomyocyte AFM assay

Abstract: A novel AFM-based assay was in agreement with traditional cytotoxicity assays and exposed a size-dependent toxicity of boron nitride nanotubes.

Cited by 26 publications

References 40 publications

Structure, Properties and Applications of Two‐Dimensional Hexagonal Boron Nitride

Structure, Properties and Applications of Two‐Dimensional Hexagonal Boron Nitride

Piezoelectric Electrospun Fibrous Scaffolds for Bone, Articular Cartilage and Osteochondral Tissue Engineering

In Vitro and In Vivo Cytotoxicity of Boron Nitride Nanotubes: A Systematic Review

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