Assessing silver nanoparticle and antimicrobial combinations for antibacterial activity and biofilm prevention on surgical sutures

Abstract

Aims To evaluate the effect of silver nanoparticles alone and in combination with Triclosan, and trans-cinnamaldehyde against S.aureus and E.coli biofilms on sutures to improve patients’ outcomes. Methods and results Silver nanoparticles were prepared by chemical method and characterized by UV–visible spectrophotometer and dynamic light scattering. The minimum inhibitory concentration was assessed by the Microdilution assay. The antibiofilm activity was determined using crystal violet assay. A checkerboard assay using the fractional inhibitory concentration index and time-kill curve was used to investigate the synergistic effect of silver nanoparticle combinations. The hemolytic activity was determined using Erythrocyte Hemolytic Assay. Our results revealed that silver nanoparticles, Triclosan, and trans-cinnamaldehyde inhibited S.aureus and E.coli biofilms. Silver nanoparticles with trans-cinnamaldehyde showed a synergistic effect (FICI values 0.35 and 0.45 against S.aureus and E.coli biofilms, respectively), and silver nanoparticles with Triclosan showed complete inhibition of S.aureus biofilm. The hemolytic activity was < 2.50% for the combinations. Conclusion and Impact of the study This study showed that combinations of trans-cinnamaldehyde and Triclosan with silver nanoparticles enhanced surgical suture performance and thus have a promising application in the prevention of surgical site infections.

Synthesis of silver nanoparticles as an antimicrobial mediator

“…Biofilms converse protection from hostile environment and could be reservoirs for pathogenic microorganisms and causes of disease epidemics, particularly in medical devices. AgNPs efficiently preclude biofilms formation and kill bacteria in developed biofilms that proposes that AgNPs can be utilized to prevent and treat biofilm-related infections [91]. Thus potential of utilizing AgNPs with enhanced bioactivity offers a promising and active alternative to traditional antimicrobial agents associated with health concerns.…”