Assessing serum cytokine profiles in inflammatory breast cancer patients using Luminex® technology

Bessaad, Maryem; Habel, Azza; Hadj Ahmed, Mariem; Xu, Weili; Stayoussef, Mouna; Bouaziz, Hanen; Hachiche, Monia; Mezlini, Amel; Larbi, Anis; Yaacoubi-Loueslati, Besma

doi:10.1016/j.cyto.2023.156409

Cited by 1 publication

(1 citation statement)

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“…VEGF, cytokines, and their relationship with breast cancer have been well studied, but tumor tissue or blood are mainly used as biomaterials [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. The content of VEGF in the tumor and blood plasma correlates with the prognosis of breast cancer, while the concentration in saliva has been practically not studied to date.…”

Section: Introductionmentioning

confidence: 99%

The Role of Salivary Vascular Endothelial Growth Factor A, Cytokines, and Amino Acids in Immunomodulation and Angiogenesis in Breast Cancer

Sarf,

Dyachenko,

Bel’skaya

2024

Biomedicines

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In this work, we focused on the analysis of VEGF content in saliva and its relationship with pro-inflammatory cytokines and amino acids involved in immunomodulation and angiogenesis in breast cancer. The study included 230 breast cancer patients, 92 patients with benign breast disease, and 59 healthy controls. Before treatment, saliva samples were obtained from all participants, and the content of VEGF and cytokines in saliva was determined by an enzyme-linked immunosorbent assay, as well as the content of amino acids by high-performance liquid chromatography. It was found that VEGF was positively correlated with the level of pro-inflammatory cytokines IL-1β (r = 0.6367), IL-6 (r = 0.3813), IL-8 (r = 0.4370), and IL-18 (r = 0.4184). Weak correlations were shown for MCP-1 (r = 0.2663) and TNF-α (r = 0.2817). For the first time, we demonstrated changes in the concentration of VEGF and related cytokines in saliva in different molecular biological subtypes of breast cancer depending on the stage of the disease, differentiation, proliferation, and metastasis to the lymph nodes. A correlation was established between the expression of VEGF and the content of aspartic acid (r = −0.3050), citrulline (r = −0.2914), and tryptophan (r = 0.3382) in saliva. It has been suggested that aspartic acid and citrulline influence the expression of VEGF via the synthesis of the signaling molecule NO, and then tryptophan ensures tolerance of the immune system to tumor cells.

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