Assessing resistance against macrocyclic lactones in gastro-intestinal nematodes in cattle using the faecal egg count reduction test and the controlled efficacy test

Graef, Jessie De; Sarre, Charlotte; Mills, BJ; Mahabir, Sean P.; Casaert, Stijn; Wilde, Nathalie De; Weyenberg, M. Van; Geldhof, Peter; Marchiondo, Alan A.; Vercruysse, Jozef; Meeus, Patrick; Claerebout, Edwin

doi:10.1016/j.vetpar.2012.04.040

Cited by 32 publications

(13 citation statements)

References 24 publications

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“…In order to confirm AR, it is desirable to have a controlled efficacy test including naïve calves experimentally infected with field isolates and control isolates with a known susceptibility status (De Graef et al, 2012). However, such tests are costly, time consuming, and need an ethical approval for use and sacrifice of laboratory animals.…”

Section: Discussionmentioning

confidence: 99%

Limited efficacy of pour-on anthelmintic treatment of cattle under Swedish field conditions

Areskog

Ljungström²,

Höglund

2013

International Journal for Parasitology: Drugs and Drug Resistan

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Section: Discussionmentioning

confidence: 99%

Limited efficacy of pour-on anthelmintic treatment of cattle under Swedish field conditions

Areskog

Ljungström²,

Höglund

2013

International Journal for Parasitology: Drugs and Drug Resistan

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“…The LMT has being used for the detection of drug resistance for IVM, MOX and the benzimidazoles in sheep, goats and cattle nematodes (Wagland et al 1992, Rabel et al 1994, Molento & Prichard 2001, Gatongi et al 2003, Kaplan et al 2007, Demeler et al 2010, Almeida et al 2012. The development of IVM resistance and also the concomitant loss of sensitivity to MOX is an ongoing debate (Conder et al 1993, Shoop et al 1993, Molento et al 1999, Ranjan et al 2002, De Graef et al 2012. El-Abdellati et al (2010) suggest that when detecting resistance to IVM the use of any type of ML's is inappropriate.…”

Section: Discussionmentioning

confidence: 99%

“…The faecal egg count reduction test (FECRT), which compares the egg counts performed preand post-treatment can define the percentage of efficacy by reducing egg numbers. Although the method is employed on all anthelmintic classes and has been commonly used to monitor resistance in nematodes, it does not measure the reduction of adult parasite number (Coles et al 2006, De Graef et al 2012, Kaplan & Vidyashankar 2012.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of resistance in a selected field strain of Haemonchus contortus to ivermectin and moxidectin using the Larval Migration on Agar Test

et al. 2013

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Haemonchus contortus is one of the most common and economically significant causes of disease in small ruminants worldwide, and the control programs of parasitic nematodes -including H. contortus -rely mostly on the use of anthelmintic drugs. The consequence of the use of this, as the sole sanitary strategy to avoid parasite infections, was the reduction of the efficacy of all chemotherapeutic products with a heavy selection for resistance. The widespread of anthelmintic resistance and the difficulty of its early diagnosis has been a major concern for the sustainable parasite management on farms. The objective of this research was to determine and compare the ivermectin (IVM) and moxidectin (MOX) effect in a selected field strain of H. contortus with a known resistance status, using the in vitro larval migration on agar test (LMAT). Third stage larvae of the selected isolate were obtained from faecal cultures of experimentally infected sheep and incubated in eleven increasing diluted concentrations of IVM and MOX (6, 12, 24, 48, 96, 192, 384, 768, 1536, 3072 and 6144µg/mL). The dose-response sigmoidal curves were obtained using the R 2 value of >0.90 and the lethal concentration (LC 50 ) dose for the tested anthelmintic drugs using a four-parameter logistic model. The LC 50 value for MOX was significantly lower than IVM (1.253µg/mL and 91.06µg/mL), identifying the H. contortus isolate as considerably less susceptible to IVM compared to MOX. Furthermore, the LMAT showed a high consistency (p<0.0001) and provided to be a useful diagnostic tool for monitoring the resistance status of IVM and MOX in H. contortus field isolate, as well as it may be used for official routine drug monitoring programs under the Ministry of Agriculture (MAPA) guidance. mais comuns e economicamente significativas de doença em produções de pequenos ruminantes em todo o mundo, e os programas de controle de parasitas nematoides -incluindo H. contortus -baseiam-se principalmente no uso de drogas anti-helmínticas. A consequência da utilização desses compostos, como sendo a única estratégia sanitária para evitar infecções por parasitas, tem sido a redução da eficácia de todos os produtos quimioterápicos, selecionando fortemente para resistência. O desenvolvimento generalizado da resistência anti-helmíntica e a dificuldade de seu diagnóstico precoce têm sido uma grande preocupação para o manejo sustentável de parasitas no campo. O obje- (6, 12, 24, 48, 96, 192, 384, 768, 1536, 3072 e 6144µg/mL). As curvas sigmoides de dose-resposta foram obtidas utilizando o valor de R 2 >0,90 e a dose de concentração letal (CL 50 ) para as drogas anti-helmínticas testadas, utilizando um modelo logístico de quatro parâ-metros. O valor de CL 50 para MOX foi significativamente menor do que para IVM (1,253µg/mL e 91,06µg/mL), identificando o isolado de H. contortus como consideravelmente menos suscetível à IVM em comparação à MOX. Além disso, o LMTA mostrou uma alta consistência (p<0,0001) e pode ser uma ferramenta útil de diagnóstico para monitorar o status de ...

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“…Calves were infected with an in-house C. oncophora strain according to De Graef et al 25 Adult-stage C. oncophora were collected from the large intestine 21 days postinfection. The worms were subsequently placed on a modified Baermann apparatus filled with physiological water at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

In-Depth Proteomic and Glycomic Analysis of the Adult-Stage Cooperia oncophora Excretome/Secretome

et al. 2013

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Cooperia oncophora is one of the most common intestinal parasitic nematodes in cattle worldwide. To date, C. oncophora infections are treated using broad-spectrum anthelmintics. However, during the past decade, reports of anthelmintic resistance in this parasite species have emerged worldwide, necessitating new avenues for its control, possibly through vaccination. In this frame, we analyzed the adult-stage C. oncophora excretome/secretome (ES), covering both the protein and glycan components, since this fraction constitutes the primary interface between parasite and host and may hold potential vaccine candidates. Two-dimensional gel electrophoretic separation of the ES material enabled the MALDI-TOF mass spectrometry (MS)-directed identification of 12 distinct proteins, grouped in three separate molecular weight fractions: (i) a high molecular weight fraction consisting of a double-domain activation-associated secreted protein (ASP), (ii) a midmolecular weight fraction predominantly containing a single-domain ASP, a thioredoxin peroxidase and innexin, and (iii) a low molecular weight protein pool essentially holding two distinct low molecular weight antigens. Further MS-driven glycan analysis mapped a variety of N-glycans to the midmolecular weight single-domain ASP, with Man6GlcNAc2 oligomannosyl glycans as the major species. The predominance of the nonglycosylated double-domain ASP in the high-molecular weight fraction renders it ideal for advancement toward vaccine trials and development.

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Assessing resistance against macrocyclic lactones in gastro-intestinal nematodes in cattle using the faecal egg count reduction test and the controlled efficacy test

Cited by 32 publications

References 24 publications

Limited efficacy of pour-on anthelmintic treatment of cattle under Swedish field conditions

Limited efficacy of pour-on anthelmintic treatment of cattle under Swedish field conditions

Evaluation of resistance in a selected field strain of Haemonchus contortus to ivermectin and moxidectin using the Larval Migration on Agar Test

In-Depth Proteomic and Glycomic Analysis of the Adult-Stage Cooperia oncophora Excretome/Secretome

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