Assessing pollution in coastal ecosystems: a preliminary survey using the micronucleus test in the mussel Mytilus edulis

Izquierdo, Jose Antonio Avilés; Machado, Gonzalo; Ayllón, Fernando; D'amico, Veronica Laura; Bala, Luis Oscar; Vallarino, Eduardo Alberto; Elías, Rodolfo; García‐Vázquez, Eva

doi:10.1016/s0147-6513(02)00041-6

Cited by 59 publications

(35 citation statements)

References 28 publications

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“…Our results show that generally metal accumulation in highest in stomach followed by muscle and gill. This was the case in many fish species, although interspecies differences were shown on the accumulation of various metals in these tissues (Izquierdo et al, 2002;Yilmaz, 2002). The levels of Zn, Cu and Pb were determined in the muscle in each species because of its importance for human consumption and also the gill and stomach were analyzed since these organs tend to accumulate metals (Marcovecchio, 2003).…”

Section: Discussionmentioning

confidence: 99%

Concentration of Zn, Cu and Pb in Some Selected Marine Fishes of the Pahang Coastal Waters, Malaysia

Kamaruzzaman¹

2010

American Journal of Applied Sciences

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Problem statement: Heavy metals constitute one of the most hazardous substances that could be accumulated in biota. Fish populations exploited by man often live in coastal area environments that contain high levels of heavy metals, coming from human activities such as industrial and agricultural wastes. A problem to deal when using fishes as biomonitors of heavy metals is the relationship existing between metal concentration and several intrinsic factors of the fish such as organism size, genetic composition and age of fish. The estimated values of all metals in muscles of fish in this study were below the established values. Therefore, it can be concluded that the fish from Pahang coastal water are comparatively clean and do not constitute a risk for human health.

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Section: Discussionmentioning

confidence: 99%

Concentration of Zn, Cu and Pb in Some Selected Marine Fishes of the Pahang Coastal Waters, Malaysia

Kamaruzzaman¹

2010

American Journal of Applied Sciences

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“…This test has an extensive record of successfully documenting elevated levels of chromosomal damage resulting from clastogens. More importantly, micronucleus frequency has been used in many wildlife ecotoxicological studies where animals are exposed to a variety of contaminants (Lyne et al, 1992;Alsabti and Metcalfe, 1995;Bickham et al, 1998b;Bresler et al, 1999;Bombail et al, 2001;Izquierdo et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal Damage in Two Species of Aquatic Turtles (Emys orbicularis and Mauremys caspica) Inhabiting Contaminated Sites in Azerbaijan

et al. 2005

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The Caspian region and specifically the Apsheron peninsula of Azerbaijan are known to be polluted with a variety of environmental contaminants. These complex mixtures of contaminants make risk assessment difficult. We used the flow cytometry method (FCM) and the micronucleus assay (MN) to assess chromosomal damage in aquatic turtles (Emys orbicularis, the European pond turtle; and Mauremys caspica, the Caspian turtle) inhabiting contaminated wetlands in Azerbaijan. Evidence of genetic damage was found for two sites, Neftchala and Sumgayit, relative to a reference site, Ali Bairamly. Sediment samples from each site were analyzed for PAHs and mercury to evaluate potential contaminant associations with genetic damage. A significant positive correlation was documented between three-ring PAH sediment concentrations and FCM estimates of chromosomal damage in E. orbicularis. These data combine to show that the contaminated wetlands in Sumgayit and Neftchala are genotoxic and that three-ring PAHs are likely a significant influence on observed genotoxicity.

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“…This result is in agreement with data obtained for Mytilus galloprovincialis [Venier et al, 1997] and Crassostrea gigas [Burgeot et al, 1995], showing a significant increase in MN formation after 48 hr of exposure with B[a]P. However, this rapid mitotic activity does not appear to induce a high MN response in the hemocytes, since the highest mean MN frequencies recorded in the current study were 14.5%. As indicated by several authors [Izquierdo et al, 2003;Bolognesi et al, 2004;Siu et al, 2004a;Carvalho Pinto-Silva et al, 2005], the low induction capability of MN appears to be a general feature of mussel hemocytes.…”

Section: Discussionmentioning

confidence: 79%

Genotoxic effects of p,p′‐DDT (1,1,1‐trichloro‐2,2‐bis‐(chlorophenyl)ethane) and its metabolites in Zebra mussel (D. polymorpha) by SCGE assay and micronucleus test

Binelli

Riva

Cogni

et al. 2008

Environ and Mol Mutagen

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This is the first study to evaluate the potential genotoxicity of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and its metabolites (p,p'-DDD and p,p'-DDE) in the sentinel mollusc Zebra mussel (Dreissena polymorpha). DNA damage was measured using the single cell gel electrophoresis (SCGE) assay and the micronucleus test (MN test), which represent two of the more sensitive biomarkers for genotoxicity evaluation. Three different concentrations (0.1, 2, and 10 mug/L) of each compound were administered in water for 168 hr, maintaining mussels at constant laboratory conditions and collecting several specimens every 48 hr for biochemical analyses. At the same time, the bioaccumulation process and the concentration/effect relationship were checked by GC-MS/MS analyses of mussel soft tissues. The SCGE assay results showed a clear and significant (P < 0.05) relationship between DNA injuries and tested doses for all the homologues throughout the 7-day exposure period. The final DNA damage due to p,p'-DDE was almost double that of the other two homologues that showed the same toxicity pattern. The micronucleus frequency analysis confirmed the genotoxicity potential of the three homologues and p,p'-DDE showed the highest irreversible DNA damage. The capability of Zebra mussels to biotransform the administered compound in the other homologues was demonstrated by multiple regression analyses carried out between the MN frequencies and the concentrations of the different homologues in the mussel soft tissues. A greater genotoxic potential of the p,p'-DDE with respect to the other two chemicals was revealed.

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Assessing pollution in coastal ecosystems: a preliminary survey using the micronucleus test in the mussel Mytilus edulis

Cited by 59 publications

References 28 publications

Concentration of Zn, Cu and Pb in Some Selected Marine Fishes of the Pahang Coastal Waters, Malaysia

Concentration of Zn, Cu and Pb in Some Selected Marine Fishes of the Pahang Coastal Waters, Malaysia

Chromosomal Damage in Two Species of Aquatic Turtles (Emys orbicularis and Mauremys caspica) Inhabiting Contaminated Sites in Azerbaijan

Genotoxic effects of p,p′‐DDT (1,1,1‐trichloro‐2,2‐bis‐(chlorophenyl)ethane) and its metabolites in Zebra mussel (D. polymorpha) by SCGE assay and micronucleus test

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