Assessing minipig compact jawbone quality at the microscale

Kochetkova, Tatiana; Groetsch, Alexander; Indermaur, Michael; Peruzzi, Cinzia; Remund, Stefan M.; Neuenschwander, Beat; Bellón, Benjamin; Michler, Johann; Zysset, Philippe; Schwiedrzik, Jakob

doi:10.1016/j.jmbbm.2022.105405

Cited by 5 publications

(2 citation statements)

References 72 publications

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“…Further details about the background subtraction and peak‐fitting steps can be found elsewhere. [ 25 , 35 ) Representative background corrected Raman spectra for each biopsy can be found in the Supporting Information (Fig. S4 ).…”

Section: Methodsmentioning

confidence: 99%

Tensile Mechanical Properties of Dry Cortical Bone Extracellular Matrix: A Comparison Among Two Osteogenesis Imperfecta and One Healthy Control Iliac Crest Biopsies

Indermaur,

Casari,

Kochetkova

et al. 2023

JBMR Plus

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Osteogenesis imperfecta (OI) is a genetic, collagen‐related bone disease that increases the incidence of bone fractures. Still, the origin of this brittle mechanical behavior remains unclear. The extracellular matrix (ECM) of OI bone exhibits a higher degree of bone mineralization (DBM), whereas compressive mechanical properties at the ECM level do not appear to be inferior to healthy bone. However, it is unknown if collagen defects alter ECM tensile properties. This study aims to quantify the tensile properties of healthy and OI bone ECM. In three transiliac biopsies (healthy n = 1, OI type I n = 1, OI type III n = 1), 23 microtensile specimens (gauge dimensions 10 × 5 × 2 μm3) were manufactured and loaded quasi‐statically under tension in vacuum condition. The resulting loading modulus and ultimate strength were extracted. Interestingly, tensile properties in OI bone ECM were not inferior compared to controls. All specimens revealed a brittle failure behavior. Fracture surfaces were graded according to their mineralized collagen fibers (MCF) orientation into axial, mixed, and transversal fracture surface types (FST). Furthermore, tissue mineral density (TMD) of the biopsy cortices was extracted from micro–computed tomogra[hy (μCT) images. Both FST and TMD are significant factors to predict loading modulus and ultimate strength with an adjusted R2 of 0.556 (p = 2.65e−05) and 0.46 (p = 2.2e−04), respectively. The influence of MCF orientation and DBM on the mechanical properties of the neighboring ECM was further verified with quantitative polarized Raman spectroscopy (qPRS) and site‐matched nanoindentation. MCF orientation and DBM were extracted from the qPRS spectrum, and a second mechanical model was developed to predict the indentation modulus with MCF orientation and DBM (R2 = 67.4%, p = 7.73e−07). The tensile mechanical properties of the cortical bone ECM of two OI iliac crest biopsies are not lower than the one from a healthy and are primarily dependent on MCF orientation and DBM. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

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Section: Methodsmentioning

confidence: 99%

Tensile Mechanical Properties of Dry Cortical Bone Extracellular Matrix: A Comparison Among Two Osteogenesis Imperfecta and One Healthy Control Iliac Crest Biopsies

Indermaur,

Casari,

Kochetkova

et al. 2023

JBMR Plus

Self Cite

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show abstract

“…All spectra were background-subtracted (second-order polynomial fit for local minima) and corrected for cosmic rays (Anton Paar instrument software v1.4 in-build function) while no smoothing was applied. Peaks of interest representing mineral and collagen content were further fit with the Lorentzian function superposition using a least square scheme (scipy.optimize.leastsq) ( Kochetkova et al, 2022 , Kochetkova et al, 2021 ). The ratio of the secondary phosphate (v 2 PO 4 , 410–460 cm −1 ) over the amide III (1215–1300 cm −1 ) bands was used as an indirect estimation of the bone mineralization level, as suggested by Roschger et al ( Roschger et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%