Assessing microstructures of the cornea with Gabor-domain optical coherence microscopy: pathway for corneal physiology and diseases

Tankam, Patrice; Hé, Zhiguo; Chu, Ying-Ju; Won, Jungeun; Canavesi, Cristina; Lépine, Thierry; Hindman, Holly B.; Topham, David J.; Gain, Philippe; Thuret, Gilles; Rolland, Jannick P.

doi:10.1364/ol.40.001113

Cited by 32 publications

(15 citation statements)

References 25 publications

(22 reference statements)

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“…For example, conventional en face OCT [21,22], rapidly evolving swept-source OCT (SS-OCT) [23][24][25] and full-field SS-OCT [26] can visualize the human cornea in vivo, but have relatively low lateral resolution. Gabor domain optical coherence microscopy (OCM) [27] and µOCT [28] were able to acquire high resolution en face images with visible stromal keratocytes and endothelial cells, however until now only from ex vivo posterior cornea. Nonlinear microscopy demonstrated high-contrast high-resolution en face images of the entire human cornea ex vivo [29] and, recently, images of the collagen fibrils in rat cornea in vivo [30], but requirements for a long acquisition time make its application limited to immobilized animal studies.…”

Section: Introductionmentioning

confidence: 99%

In vivo high resolution human corneal imaging using full-field optical coherence tomography

Mazlin¹,

Xiao²,

Dalimier³

et al. 2018

Biomed. Opt. Express

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We present the first full-field optical coherence tomography (FFOCT) device capable of imaging of the human cornea. We obtained images of the epithelial structures, Bowman's layer, sub-basal nerve plexus (SNP), anterior and posterior stromal keratocytes, stromal nerves, Descemet's membrane and endothelial cells with visible nuclei. Images were acquired with a high lateral resolution of 1.7 µm and relatively large field-of-view of 1.26 mm x 1.26 mm - a combination, which, to the best of our knowledge, has not been possible with other human eye imaging methods. The latter together with a contactless operation, make FFOCT a promising candidate for becoming a new tool in ophthalmic diagnostics.

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Section: Introductionmentioning

confidence: 99%

In vivo high resolution human corneal imaging using full-field optical coherence tomography

Mazlin¹,

Xiao²,

Dalimier³

et al. 2018

Biomed. Opt. Express

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“…This limits the lateral resolution to values in the range of 15 to 20 µm, hampering imaging on a cellular level. Using a liquid lens for dynamic focusing on different depth locations [30], Gabor-domain OCM allowed to overcome this limitation and provided non-contact ex vivo imaging of corneal cells in various layers and corneal nerves in the central cornea, comparable to CM [31].…”

Section: Introductionmentioning

confidence: 99%

Ultrahigh-resolution OCT imaging of the human cornea

Werkmeister¹,

Sapeta²,

Schmidl³

et al. 2017

Biomed. Opt. Express

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We present imaging of corneal pathologies using optical coherence tomography (OCT) with high resolution. To this end, an ultrahigh-resolution spectral domain OCT (UHR-OCT) system based on a broad bandwidth Ti:sapphire laser is employed. With a central wavelength of 800 nm, the imaging device allows to acquire OCT data at the central, paracentral and peripheral cornea as well as the limbal region with 1.2 µm x 20 µm (axial x lateral) resolution at a rate of 140 000 A-scans/s. Structures of the anterior segment of the eye, not accessible with commercial OCT systems, are visualized. These include corneal nerves, limbal palisades of Vogt as well as several corneal pathologies. Cases such as keratoconus and Fuchs's endothelial dystrophy as well as infectious changes caused by diseases like Acanthamoeba keratitis and scarring after herpetic keratitis are presented. We also demonstrate the applicability of our system to visualize epithelial erosion and intracorneal foreign body after corneal trauma as well as chemical burns. Finally, results after Descemet's membrane endothelial keratoplasty (DMEK) are imaged. These clinical cases show the potential of UHR-OCT to help in clinical decision-making and follow-up. Our results and experience indicate that UHR-OCT of the cornea is a promising technique for the use in clinical practice, but can also help to gain novel insight in the physiology and pathophysiology of the human cornea.

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“…The outcome of OCT based imaging is constantly developing with further iterations on the technology. In fact, the latest iterations include Gabor-domain optical coherence microscopy which can be useful in assessment of the cornea [5,6]. Based on this understanding, the aforementioned changes can be detected early by imaging the macula using an Ocular Coherence Tomography that facilitates both thicknesses and morphology detection before these changes are clinically apparent.…”

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confidence: 99%

Normative values of the retinal macular thickness in a middle eastern population

2020

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Background: Since the normative value of the retinal macular thickness is undocumented in the Middle East, the aim of this work is to assess the normative values of the macular thickness in healthy eyes in a Middle Eastern population and its relationship with age, sex, and laterality. Methods: One hundred sixteen individuals were randomly selected from volunteers visiting the Jordan University Hospital in Amman, Jordan. Measurements were obtained using the Fourier domain optical coherence tomography (OCT). Multivariate regression models were developed to obtain predicted normative values with adjustment to candidate variables. In addition, the effect of age, sex and laterality were evaluated. Results: The average central fovea macular thickness was 229.5 (±30.85) um. The quadratic value of the retinal macular thickness decreased from the superior value of 299.71 (±23.67) um (P = .001) to the inferior value of 296.46 (±28.85) um(P = .001) and a nasal figure of 93.63 (±26.86) um(P = .001). The temporal area has the thinnest value of 293.43 (±30.78) um (P = 0.001). Central thickness was higher in males with a mean variation of 11.67 um (95% CI, 2.41 to 20.93) (p = 0.003). The thickness was highest within 3 mm diameter from the center and decreased towards the periphery Eye sidedness didn't contribute to variability of the macular thickness. Furthermore, we found a significant difference between age and central macular thickness (p = 0.001), as age was a positive predictor for macular thickness. Conclusion: Our set of predicted normative data may be used to interrupt measurement of the macular thickness in Middle Eastern population. The average fovea macular thickness among Jordanians is consistent with previously reported values. Normative values from additional Middle Eastern. Population are required to appraise our model.

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Assessing microstructures of the cornea with Gabor-domain optical coherence microscopy: pathway for corneal physiology and diseases

Cited by 32 publications

References 25 publications

In vivo high resolution human corneal imaging using full-field optical coherence tomography

In vivo high resolution human corneal imaging using full-field optical coherence tomography

Ultrahigh-resolution OCT imaging of the human cornea

Normative values of the retinal macular thickness in a middle eastern population

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