Assessing lethal and sub-lethal effects of trichlorfon on different trophic levels

Coelho, Sónia D.; Oliveira, Rhaul; Pereira, Sara; Musso, Carolina; Domingues, Inês; Bhujel, Ram C.; Soares, Amadeu M.V.M.; Nogueira, António J.A.

doi:10.1016/j.aquatox.2011.03.003

Cited by 75 publications

(33 citation statements)

References 41 publications

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“…They feed on phytoplankton and are preyed upon by aquatic vertebrates and macro-invertebrates. The unique responses of ChE levels in Daphnia when exposed to anticholinesterases have been observed multiple times (Gälli et al, 1994;Guilhermino et al, 1996;Sturm and Hansen, 1999;Barata et al, 2001;Carvalho et al, 2003;Duquesne, 2006;Vesela et al, 2006;Dámasio et al, 2007;Jemec et al, 2007;Printes et al, 2008;Duquesne and Küster, 2010;Coelho et al, 2011;Li and Tan, 2011). According to Sturm and Hansen (1999), up to 80% ChE inhibition was observed in Daphnia after the animals were exposed for 24 h to dichlorvos, parathion, and aldicarb, respectively, at concentration of 0.25, 1, and 250 μg/L.…”

Section: Introductionmentioning

confidence: 99%

“…It is known that acute toxicities of anticholinesterases could be two orders of magnitude higher in Daphnia than in aquatic vertebrates such as fish and amphibians. For example, according to Coelho et al (2011), the 48-h LC 50 of trichlorfon for Daphnia magna was 0.29 μg/L, whereas 96-h LC 50 of the same insecticide for early-life stage and adult zebrafish was 25.4×10 3 and 28.8×10 3 μg/L, respectively. Studies on crude extracts indicated that the ChE from Daphnia magna showed characteristics of both AChE and PChE (Diamantino et al, 2007;Vesela et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile the authors found 24-h LC 50 values (lethal concentration that kills 50% of the test animals in a given time) of the three insecticides were 0.233, 2.35, and 227.6 μg/L, respectively. A recent study conducted by Coelho et al (2011) revealed an 88% ChE activity inhibition in Daphnia exposed for 48 h to trichlorfon of 0.25 μg/L, while the study revealed a 48-h LC 50 of 0.29 μg/L. A study conducted by Duquesne and Küster (2010) revealed 80% ChE activity inhibition in Daphnia being exposed to 1.5 μg/L parathion-methyl for 24 h while the paper revealed that the insecticide had a 48-h LC 50 of 0.6-7.0 μg/L.…”

Section: Introductionmentioning

confidence: 99%

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Purification and studies on characteristics of cholinesterases from Daphnia magna

Yang

Niu

2013

J. Zhejiang Univ. Sci. B

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Due to their significant value in both economy and ecology, Daphnia had long been employed to investigate in vivo response of cholinesterase (ChE) in anticholinesterase exposures, whereas the type constitution and property of the enzyme remained unclear. A type of ChE was purified from Daphnia magna using a three-step procedure, i.e., Triton X-100 extraction, ammonium sulfate precipitation, and diethylaminoethyl (DEAE)-Sepharose™-Fast-Flow chromatography. According to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), molecular mass of the purified ChE was estimated to be 84 kDa. Based on substrate studies, the purified enzyme preferred butyrylthiocholine iodide (BTCh) [with maximum velocity (V max )/Michaelis constant (K m )=8.428 L/(min·mg protein)] to acetylthiocholine iodide (ATCh) [with V max /K m =5.346 L/(min·mg protein)] as its substrate. Activity of the purified enzyme was suppressed by high concentrations of either ATCh or BTCh. Inhibitor studies showed that the purified enzyme was more sensitive towards inhibition by tetraisopropylpyrophosphoramide (iso-OMPA) than by 1,5-bis(4-allyldimethylammoniumphenyl) pentan-3-one dibromide (BW284C51). Result of the study suggested that the purified ChE was more like a type of pseudocholinesterase, and it also suggested that Daphnia magna contained multiple types of ChE in their bodies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Purification and studies on characteristics of cholinesterases from Daphnia magna

Yang

Niu

2013

J. Zhejiang Univ. Sci. B

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“…It consumes algae and other small phytoplanktons and in turn acts as prey for freshwater fish and large aquatic invertebrates. Due to its worldwide distribution and the importance of the species in maintaining the sustainability of aquatic ecosystems, the animal has long been noted for its responses in terms of ChE during anticholinesterase exposure (Gälli et al, 1994;Guilhermino et al, 1996;Barata et al, 2001;Carvalho et al, 2003;Duquesne, 2006;Vesela et al, 2006;Damásio et al, 2007;Jemec et al, 2007;Printes et al, 2008;Duquesne and Küster, 2010;Coelho et al, 2011;Li and Tan, 2011).…”

Section: Introductionmentioning

confidence: 99%

Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna

Liu

Yuan

2016

J. Zhejiang Univ. Sci. B

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Abstract:To yield cholinesterase (ChE) from prokaryotic expression, the ChE gene that belongs to Daphnia magna was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using forward primer 5'-CCCYGGNGCSAT GATGTG-3' and reverse primer 5'-GYAAGTTRGCCCAATATCT-3'. To express the gene, one sequence of the amplified DNA, which was able to encode a putative protein containing two conserved carboxylesterase domains, was connected to the prokaryotic expression vector PET-29a(+). The recombinant vector was transformed into Escherichia coil BL21 (DE3). Protein expression was induced by isopropy-D-thiogalactoside. The expressed ChE was used as an immunogen to immunize BALB/c mice. The obtained antibodies were tested for their specificity towards crude enzymes from species such as Alona milleri, Macrobrachium nipponense, Bombyx mori, Chironomus kiiensis, Apis mellifera, Eisenia foetida, Brachydanio rerio, and Xenopus laevis. Results indicated that the antibodies had specificity suitable for detecting ChE in Daphnia magna. A type of indirect and non-competitive enzyme-linked immunosorbent assay (IN-ELISA) was used to test the immunoreactive content of ChE (ChE-IR) in Daphina magna. The detection limit of the IN-ELISA was found to be 14.5 ng/ml at an antiserum dilution of 1:22 000. Results from tests on Daphnia magna exposed to sublethal concentrations of triazophos indicated a maximal induction of 57.2% in terms of ChE-IR on the second day after the animals were exposed to a concentration of 2.10 μg/L triazophos. Testing on animals acclimatized to a temperature of 16 °C indicated that ChE-IR was induced by 16.9% compared with the ChE-IR content detected at 21 °C, and the rate of induction was 25.6% at 10 °C. The IN-ELISA was also used to test the stability of ChE-IR in collected samples. Repeated freezing and thawing had no influence on the outcome of the test. All these results suggest that the polyclonal antibodies developed against the recombinant ChE are as efficient as those developed against the native ChE in detecting ChE content in Daphnia magna.

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“…Aquatic animals have often been used in bioassay to monitor water quality of effluent and surface waters (Bruges et al, 1977). Organophosphate (OP) pesticides are used worldwide to control arthropod pests in agriculture and aquaculture, and often end up polluting waterways (Coelho et al, 2011, Jordaan et al, 2013Tierney et al, 2007;Var? et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of Lannat Pesticide on Growth Performance and Chromosomal Aberrations in Nile Tilapia (Oreochromis Niloticus L.)

Khaled

2017

Alexandria Science Exchange Journal

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Nile Tilapia, (Oreochromis niloticus L.,) is the most popular animal protein for Egyptian people due to their price and availability. Todays, a ll the production fish exposed to biotic and abiotic environmental stress, that cause huge damage for the fish and costumers. During the current research one common pesticide Lannat was used by different concentrations i.e. 0.8, 1.6 and 3.2 ppm, to investigate the possible genotoxic effect in aquatic organisms, measurement and alteration by the physicochemical properties of DNA and isolated chromatin from liver tissue and finally, investigating the chromosomal aberrations in vivo induction of sister chromatid exchange (SCEs). This work was carried out at the faculty of Agriculture Saba Basha during the period 2016-2017. The results showed that Mitotic activity in the gills of the control group was higher than in kidneys. In treatments, cells of gills displayed lower mitotic activity compared with that of kidneys. Different mitotic aberrations were recorded with high doses of Lannat such as stickiness and ring chromosomes, deletion, fragments, and Robertsonian Centric Fusion (RCF). Lannat showed that it can induce macro-DNA damage. Lysis of nucleus was also observed.

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Assessing lethal and sub-lethal effects of trichlorfon on different trophic levels

Cited by 75 publications

References 41 publications

Purification and studies on characteristics of cholinesterases from Daphnia magna

Purification and studies on characteristics of cholinesterases from Daphnia magna

Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna

Effect of Lannat Pesticide on Growth Performance and Chromosomal Aberrations in Nile Tilapia (Oreochromis Niloticus L.)

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