Assessing Land Use and Biodiversity Impacts of Industrial Biotechnology

Lindner, Jan Paul; Beck, Tabea; Bos, Ulrike; Albrecht, Stefan

doi:10.1007/10_2019_114

Cited by 5 publications

(1 citation statement)

References 41 publications

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“…Microorganisms, in particular, possess unique properties that enable the production of complex multicarbon compounds with high efficiency under mild reaction conditions. However, most microbial production processes currently rely on glucose as a carbon source, which requires arable land and thus potentially competes with the food sector [5]. This is particularly problematic if increasing quantities of sugars were to be used for producing large quantities of bulk chemicals.…”

Section: Introductionmentioning

confidence: 99%

Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha

Wefelmeier,

Schmitz,

Kösters

et al. 2024

Microb Cell Fact

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Background One carbon (C1) molecules such as methanol have the potential to become sustainable feedstocks for biotechnological processes, as they can be derived from CO2 and green hydrogen, without the need for arable land. Therefore, we investigated the suitability of the methylotrophic yeast Ogataea polymorpha as a potential production organism for platform chemicals derived from methanol. We selected acetone, malate, and isoprene as industrially relevant products to demonstrate the production of compounds with 3, 4, or 5 carbon atoms, respectively. Results We successfully engineered O. polymorpha for the production of all three molecules and demonstrated their production using methanol as carbon source. We showed that the metabolism of O. polymorpha is well suited to produce malate as a product and demonstrated that the introduction of an efficient malate transporter is essential for malate production from methanol. Through optimization of the cultivation conditions in shake flasks, which included pH regulation and constant substrate feeding, we were able to achieve a maximum titer of 13 g/L malate with a production rate of 3.3 g/L/d using methanol as carbon source. We further demonstrated the production of acetone and isoprene as additional heterologous products in O. polymorpha, with maximum titers of 13.6 mg/L and 4.4 mg/L, respectively. Conclusion These findings highlight how O. polymorpha has the potential to be applied as a versatile cell factory and contribute to the limited knowledge on how methylotrophic yeasts can be used for the production of low molecular weight biochemicals from methanol. Thus, this study can serve as a point of reference for future metabolic engineering in O. polymorpha and process optimization efforts to boost the production of platform chemicals from renewable C1 carbon sources.

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Section: Introductionmentioning

confidence: 99%