Assessing indocyanine green pharmacokinetics in mouse liver with a dynamic diffuse fluorescence tomography system

Zhang, Yanqi; Zhang, Limin; Yin, Guoyan; Ma, Wenjuan; Gao, Feng

doi:10.1002/jbio.201800041

Cited by 10 publications

(14 citation statements)

References 45 publications

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“…ICG enters hepatocytes of normal liver tissue quickly and emits fluorescence, the intensity of which gradually weakens after excretion into bile (11). The detected fluorescence intensity of ICG is eliminated in a typical linear manner, which can be used to evaluate liver reserve function after intravenously injection at a high dose (12).…”

Section: Discussionmentioning

confidence: 99%

Assessment of liver injury using indocyanine green fluorescence imaging

Chen¹,

Liu²,

Wu³

et al. 2021

Ann Transl Med

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Background: To investigate whether indocyanine green (ICG) fluorescence imaging can be used to evaluate chronic and acute liver injury induced by either a high-fat (HF) diet or carbon tetrachloride (CCl 4 ).Methods: Sprague-Dawley (SD) rats were randomly divided into three groups: control group, HF diet-induced model group, and CCl 4 -induced model group. The chronic and acute liver injury models were induced by a HF diet and intraperitoneal injection of CCl 4 , respectively. After HF feeding, the liver index, levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of the rats were determined. The livers were also collected to evaluate histopathology damage by hematoxylin and eosin (H&E) staining. After in vitro perfusion of the liver and ICG administration, the liver fluorescence intensity and corresponding spectral value were measured by using real-image guided system (REAL-IGS).Results: After HF feeding, the liver index and levels of serum ALT and AST were significantly increased, and the livers of the rats showed severe histopathological changes. Compared with the control group, the hepatic lobes of the model rats exhibited incomplete green fluorescence, and the corresponding spectral value was markedly reduced.Conclusions: ICG fluorescence imaging can be used to evaluate liver injury induced by either a HF diet or CCl 4 .

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Section: Discussionmentioning

confidence: 99%

Assessment of liver injury using indocyanine green fluorescence imaging

Chen¹,

Liu²,

Wu³

et al. 2021

Ann Transl Med

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“…Generally, DFT systems can probe light by optical fibers combined with highly sensitive photoelectric sensors [photomultiplier tubes (PMT) or avalanche photodiodes], or alternatively by sensitive charge-coupled device (CCD) cameras. [13][14][15][16][17][18][19][20] Compared with CCD camera detection mode, fiber-based detection combining with high-sensitive photoelectric sensors and photoncounting technique allows higher detection sensitivity and larger dynamic measurement range, which is significantly beneficial to probing ICG suffering low quantum yield, especially after a period of metabolism in tissue. At present, the pharmacokinetics of ICG has been studied to assess tumor issues and mouse liver function with the support of fiber-based DFT systems.…”

Section: Introductionmentioning

confidence: 99%

High-sensitivity dynamic diffuse fluorescence tomography system for fluorescence pharmacokinetics

et al. 2022

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Significance: Dynamic diffuse fluorescence tomography (DFT) can recover the static distribution of fluorophores and track dynamic temporal events related to physiological and disease progression. Dynamic imaging indocyanine green (ICG) approved by the food and drug administration is still under-exploited because of its characteristics of low quantum yield and relatively rapid tissue metabolism.Aim: In order to acquire the ICG tomographic image sequences for pharmacokinetic analysis, a dynamic DFT system was proposed.Approach: A fiber-based dynamic DFT system adopts square-wave modulation lock-in photoncounting scheme and series-parallel measurement mode, which possesses high sensitivity, large dynamic range, high anti-ambient light ability in common knowledge, as well as good cost performance. In order to investigate the effectiveness of the proposed system, the measurement stability and the anti-crosstalk-a crucial factor affecting the system parallelization-were assessed firstly, then a series of static phantoms, dynamic phantoms and in vivo mice experiments were conducted to verify the imaging capability. Results:The system has the limited dynamic range of 100 dB, the fluctuation of photon counting within 3%, and channel-to-channel crosstalk ratio better than 1.35. Under the condition of a sufficient signal-to-noise ratio, a complete measurement time for one frame image was 10.08 s. The experimental results of static phantoms with a single target and three targets showed that this system can accurately obtain the positions, sizes, and shapes of the targets and the reconstructed images exhibited a high quantitativeness. Further, the self-designed dynamic phantom experiments demonstrated the capability of the system to capture fast changing fluorescence signals. Finally, the in vivo experiments validated the practical capability of the system to effectively track the ICG metabolism in living mice.Conclusions: These results demonstrate that our proposed system can be utilized for assessing ICG pharmacokinetics, which may provide a valuable tool for tumor detection, drug assessment, and liver function evaluation.

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“…The accumulation of HFn-ICG in different organs of a breast cancer murine model was determined by using IVIS ® Lumina II in-vivo Imaging System ( Sevieri et al, 2021 ) . For fluorescence pharmacokinetic rates of ICG in mouse liver, within the in vivo imaging techniques, diffusive fluorescence tomography has been also used ( Zhang et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Bioanalytical UHPLC-MS/MS Method Applied to Murine Liver Tissue for the Determination of Indocyanine Green Loaded in H-Ferritin Nanoparticles

et al. 2022

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Indocyanine green (ICG) is one of the most commonly used fluorophores in near-infrared fluorescence-guided techniques. However, the molecule is prone to form aggregates in saline solution with a limited photostability and a moderate fluorescence yield. ICG was thus formulated using protein-based nanoparticles of H-ferritin (HFn) in order to generate a new nanostructure, HFn-ICG. In this study, an ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) system was employed to develop and validate the quantitative analysis of ICG in liver tissue samples from HFn-ICG-treated mice. To precipitate HFn, cold acetone in acidic solution at pH 5.0 was used. The processed liver samples were injected into the UHPLC-MS/MS system for analysis using the positive electrospray ionization mode. Chromatographic separation was achieved on a Waters Acquity UPLC® HSS T3 Column (1.8 μm, 2.1 × 100 mm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. The selected reaction monitoring transitions of m/z 753 →m/z 330 and m/z 827 →m/z 330 were applied for ICG and IR-820 (the internal standard, IS), respectively. The method was selective and linear over a concentration range of 50–1,500 ng/ml. The method was validated for sensitivity, accuracy, precision, extraction recovery, matrix effect, and stability in liver tissue homogenates. ICG extraction recoveries ranged between 85 and 108%. The intra- and inter-day precisions were less than 6.28%. The method was applied to a bio-distribution study to compare the amount of ICG levels from mice treated with HFn-ICG and free ICG. The analyses of the homogenate samples from the two types of treatment showed that the concentration levels of ICG is approximately six-fold higher than those of free ICG (1,411 ± 7.62 ng/ml vs. 235 ± 26.0 ng/ml) at 2 h post injection.

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Assessing indocyanine green pharmacokinetics in mouse liver with a dynamic diffuse fluorescence tomography system

Cited by 10 publications

References 45 publications

Assessment of liver injury using indocyanine green fluorescence imaging

Assessment of liver injury using indocyanine green fluorescence imaging

High-sensitivity dynamic diffuse fluorescence tomography system for fluorescence pharmacokinetics

Development and Validation of a Bioanalytical UHPLC-MS/MS Method Applied to Murine Liver Tissue for the Determination of Indocyanine Green Loaded in H-Ferritin Nanoparticles

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