Assessing genetic diversity and population structure in a Dipteryx alata germplasm collection utilizing microsatellite markers

Abstract

Dipteryx alata Vogel (Leguminosae) is a native Neotropical tree with a wide distribution in the Brazilian Cerrado that is commonly known as the baru tree. The genetic diversity of 150 D. alata progeny from a germplasm collection was characterized using nine microsatellite markers. Genetic diversity analysis detected 50 alleles ranging from 2 to 14 alleles per locus. The genetic differentiation among populations (ϑ p = 0.097) suggests moderate genetic structuring and high genetic differentiation among progenies (ϑ s = 0.169). The intrapopulation index (f = 0.122) indicates the presence of low endogamy. The effective population size (N e = 96) shows that the germplasm collection has sufficient representativeness for use as a base population for breeding programmes. These results are useful for the exploitation of the genetic resources of D. alata for future conservation efforts and breeding programmes.

Insight into the Current Genetic Diversity and Population Structure of Domestic Reindeer (Rangifer tarandus) in Russia

“…Evaluating the inbreeding and genetic diversity within a breed is a requisite for sustainable improvement in the long term [66]. In our study, we noted that the observed heterozygosity for all groups was practically close to the expected heterozygosity (Table 1), which denotes that the studied breeds were found to be under the Hardy-Weinberg (HW) equilibrium [67]. This assumption was additionally evidenced by a barely positive average inbreeding index, and the values of the confidence interval were almost tending toward zero, indicating the state of the genetic equilibrium in the reindeer groups.…”

The chloroplast genome sequence of Dipteryx alata Vog. (Fabaceae: Papilionoideae): genomic features and comparative analysis with other legume genomes

“…Although D. alata is considered a valuable genetic resource for Cerrado, few genetic investigations are available and genomic studies are rare. Some scientific articles for D. alata are found in the literature focusing on the development of small-scale molecular markers and population studies (Soares et al 2008(Soares et al , 2012(Soares et al , 2015Collevatti et al 2013;Guimarães et al 2017Guimarães et al , 2019a. In this scenario, there is a need for use and conservation purposes, to obtain new genomic knowledge for D. alata.…”

Research Article Genome size and chromosome number of <i>Dipteryx</i> <i>alata</i> (Leguminosae): a model candidate for comparative genomics in Papilionoideae

“…Samples of leaves of D. alata were collected from three specimens of the germplasm collection maintained by the Agronomy School of the Federal University of Goiás. This germplasm collection was planted in 2011 (latitude 16°35'58.96" S, longitude 49°16'49.55" W, altitude 736 m) and has 600 individual accesses from 25 populations of D. alata sampled in Cerrado regions in the Brazilian states of Goiás, Mato Grosso, Tocantins, Bahia and Minas Gerais (Guimarães et al, 2019a). For an internal standard, leaves of Glycine max (2C-Value = 2.5 pg, Graham et al, 1994) from germinated seeds supplied by Dr. Jaroslav Doležel (Experimental Institute of Botany, Czech Republic) were used to obtain a genome size estimation.…”