Assessing gene expression in vivo: magnetic resonance imaging and spectroscopy

Bell, Jimmy D.; Taylor‐Robinson, Simon D.

doi:10.1038/sj.gt.3301218

Cited by 44 publications

(21 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…23 In addition, nonradioactive strategies to visualize the expression of certain transgenes following gene transfer are being developed. 24 Interestingly, we detected statistically significant differences in the L/H ratios between AdCMV.LDLR-and control virus-injected rabbits throughout day 30 after adenovirus administration. At this time point, plasma total cholesterol was already normalized.…”

Section: Discussionmentioning

confidence: 69%

A sensitive noninvasive method for monitoring successful liver-directed gene transfer of the low-density lipoprotein receptor in Watanabe hyperlipidemic rabbits in vivo

et al. 2004

View full text Add to dashboard Cite

Noninvasive tools to quantitate transgene expression directly are a prerequisite for clinical gene therapy. We established a method to determine location, magnitude, and duration of low-densitiy lipoprotein (LDL) receptor (LDLR) transgene expression after adenoviral gene transfer into LDLR-deficient Watanabe hypercholesterolemic rabbits by following tissue uptake of intravenously injected 111 In-labeled LDL using a scintillation camera. Liver-specific tracer uptake was calculated by normalizing the counts measured over the liver to counts measured over the heart that represent the circulating blood pool of the tracer (liver/heart (L/H) ratio). Our results indicate that the optimal time point for transgene imaging is 4 h after the tracer injection. Compared with control virusinjected rabbits, animals treated with the LDLR-expressing adenovirus showed seven-fold higher L/H ratios on day 6 after gene transfer, and had still 4.5-fold higher L/H ratios on day 30. This imaging method might be a useful strategy to obtain reliable data on functional transgene expression in clinical gene therapy trials of familial hypercholesterolemia.

show abstract

Section: Discussionmentioning

confidence: 69%

A sensitive noninvasive method for monitoring successful liver-directed gene transfer of the low-density lipoprotein receptor in Watanabe hyperlipidemic rabbits in vivo

et al. 2004

View full text Add to dashboard Cite

show abstract

“…The contrast agent we used is a safe and widely used product. More sophisticated agents are now developed to assess gene expression by using MRI, 37 but are very specific for a few genes and not yet validated for medical use. The trapped quantity of the CA, although exploring only the first step of delivery of the therapeutic agent, is correlated to gene expression.…”

Section: Toxicity Of the Camentioning

confidence: 99%

In vivo NMR imaging evaluation of efficiency and toxicity of gene electrotransfer in rat muscle

et al. 2005

View full text Add to dashboard Cite

In vivo gene electrotransfer (ET) is a simple method of gene delivery in various tissues relying on the injection of plasmid DNA followed by application of electric pulses. Noninvasive tools are needed to evaluate the ET efficiency and the resulting tissue damages. In this study, we performed ET of rat tibialis muscle after injection of either a plasmid coding for luciferase or a contrast agent (CA) detected by using magnetic resonance imaging (MRI). Plasmid expression and CA intracellular trapped quantity were compared throughout the electric field intensity range 0-300 V/cm. Although the CA trapped quantity reflects only the electropermeabilization step, both measurements were correlated. MRI measurements gave easy access to tridimensional visualization of the labelled zones where the CA has been injected and the applied electric field had a value allowing permeabilization. We also performed MRI measurements of the water transverse relaxation time T2 as an indicator of tissue modification, and tested whether another CA specific for necrosis could be used to detect muscle necrosis at high electric field intensity. In conclusion, MRI measurements may bring multiparametric information upon the efficiency and tissue toxicity of an ET protocol by using a simple and safe CA.

show abstract

“…Expression varies with time and between tissues. MR techniques using MR spectroscopy and/or MR imaging, offer non-invasive methods of monitoring expression [45,46] . As in drug monitoring techniques where a MR detectable moiety or metabolite is linked to the active drug, genes expressing markers may be combined with the therapeutic gene.…”

Section: Gene Therapymentioning

confidence: 99%

Current and future applications ofin vitromagnetic resonance spectroscopy in hepatobiliary disease

Cox

Sharif²,

Cobbold³

et al. 2006

WJG

View full text Add to dashboard Cite

Nuclear magnetic resonance spectroscopy allows the study of cellular biochemistry and metabolism, both in the whole body in vivo and at higher magnetic field strengths in vitro . Since the technique is non-invasive and non-selective, magnetic resonance spectroscopy methodologies have been widely applied in biochemistry and medicine. In vitro magnetic resonance spectroscopy studies of cells, body fluids and tissues have been used in medical biochemistry to investigate pathophysiological processes and more recently, the technique has been used by physicians to determine disease abnormalities in vivo . This highlighted topic illustrates the potential of in vitro magnetic resonance spectroscopy in studying the hepatobiliary system. The role of in vitro proton and phosphorus magnetic resonance spectroscopy in the study of malignant and non-malignant liver disease and bile composition studies are discussed, particularly with reference to correlative in vivo whole-body magnetic resonance spectroscopy applications. In summary, magnetic resonance spectroscopy techniques can provide non-invasive biochemical information on disease severity and pointers to underlying pathophysiological processes. Magnetic resonance spectroscopy holds potential promise as a screening tool for disease biomarkers, as well as assessing therapeutic response.

show abstract

Assessing gene expression in vivo: magnetic resonance imaging and spectroscopy

Cited by 44 publications

References 38 publications

A sensitive noninvasive method for monitoring successful liver-directed gene transfer of the low-density lipoprotein receptor in Watanabe hyperlipidemic rabbits in vivo

A sensitive noninvasive method for monitoring successful liver-directed gene transfer of the low-density lipoprotein receptor in Watanabe hyperlipidemic rabbits in vivo

In vivo NMR imaging evaluation of efficiency and toxicity of gene electrotransfer in rat muscle

Current and future applications ofin vitromagnetic resonance spectroscopy in hepatobiliary disease

Contact Info

Product

Resources

About