Globally, human activities have a significant impact on the diversity, abundance, and distribution of large mammals in Protected Areas (PAs). These disturbances increase human pressure on biodiversity and species habitats, highlighting the need for conservation. This study aimed to assess the abundance and distribution of large mammals in different habitat types within Nimule National Park (NNP) and understand the impacts of human activities on them. Data on the abundance and distribution of large mammals and their respective habitat types were collected through line transect surveys. Human activity signs were observed and recorded along the transect lines. To estimate the impacts of human activities on the diversity, abundance, and distribution of large mammal species, as well as to identify any significant differences between them and their habitat types, the study utilized the Kruskal Wallis test, Polynomial multiple regressions, and diversity indices. The findings from the Shannon-Weiner and Simpson indices indicated that large mammal species were more diverse inside the park (H' = 1.136; D = 0.570) compared to the buffer zone (H' = 0.413; D = 0.171), with 85% (443 out of 510 samples) recorded within Nimule National Park. The species abundance showed a semi-balanced status (0.58). The diversity results among different habitat types revealed that large mammals were more diverse and highly distributed in both open woodlands (244) and dense woodlands (192), while riverine vegetation had the lowest diversity (8). Statistical tests demonstrated a highly significant difference at a 99% confidence interval (p-value = 0.01) between habitat types and identified species of large mammals.