Assessing fluorescence detection and effective photothermal therapy of near‐infrared polymer nanoparticles using alginate tissue phantoms

McCabe-Lankford, Eleanor; Brown, Theodore L.; Levi‐Polyachenko, Nicole

doi:10.1002/lsm.22955

Cited by 10 publications

(5 citation statements)

References 58 publications

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“…HDAPPs were utilized to evaluate photothermal-nanoparticlemediated hyperthermia with luciferase-based dosimetry in both CT26 parental and OxR cells. Absorption and fluorescence of HDAPPs are shown in Figure 2(A), where HDAPPs display a fluorescence quantum yield of 0.63%, which is sufficient to detect through 6 mm of tissue as shown by previous work in both tissue phantoms and in vivo [42,43]. The low quantum yield is indicative of a higher photothermal conversion efficiency (PCE), which was determined to be 51.2%.…”

Section: Demonstration Of Luminescent Monitoring Of Cell Effective Thmentioning

confidence: 55%

Oxaliplatin-resistant colorectal cancer models for nanoparticle hyperthermia

McCarthy

Singh

Levi‐Polyachenko

2021

International Journal of Hyperthermia

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Introduction: Metastatic colorectal cancer (CRC) is complicated by chemotherapy-resistant cell populations. Oxaliplatin is used in heated intraperitoneal hyperthermic chemoperfusion (HIPEC) for treatment of disseminated CRC. Photothermal nanoparticles can provide focal heating to improve the response of CRC cells to oxaliplatin, by confining heating near individual cells. Reduction in cellular luciferase signal may allow single-cell-resolution recording of thermal dosimetry. Methods: Oxaliplatin resistant (OxR) variants of luciferase-expressing CT26.WT-Fluc-Neo CRC cells were developed and their sensitivity to hyperthermia was evaluated. Polymer-based photothermal nanoparticles were developed, characterized and used to explore their potential for imparting a thermal dose to improve cell response to oxaliplatin. A correlation of thermal dose to intracellular luciferase activity was established using quantitative luminescence monitoring and microscopy. Results: Luciferase-based monitoring of thermal dose within CT26 cell lines was validated within the ranges of 0.04-8.33 CEM43 for parental cells and 0.05-9.74 CEM43 for OxR CT26 cells. This was further confirmed using nanoparticle-induced hyperthermia, where the single-cell resolution of the thermal dose can be achieved. The nanoparticles enhance cell killing of resistant cells when combined with oxaliplatin and stimulated to generate heat. Conclusion: Nanoparticle-based hyperthermia is effective for augmenting chemotherapy and can be coupled with reductions in CT26 luciferase expression to monitor thermal dose at single-cell resolution. The development of OxR CT26.WT-Fluc-Neo CRC cells sets the stage for pre-clinical evaluations to measure nanoparticle-induced hyperthermia to augment chemotherapy (Nano-HIPEC) in a chemotherapy-resistant model of disseminated CRC.

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Section: Demonstration Of Luminescent Monitoring Of Cell Effective Thmentioning

confidence: 55%

Oxaliplatin-resistant colorectal cancer models for nanoparticle hyperthermia

McCarthy

Singh

Levi‐Polyachenko

2021

International Journal of Hyperthermia

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“…Higher concentrations of AgNPs get much hotter (up to 37 + 31 • C = 68 • C). PTT can be induced rapidly, in only 36 s, using a higher laser power that will have improved penetration depth [66]. A limitation is that PTT temperatures were measured as a volumetric change (200 µL) and temperatures that the cancer or bacterial cells experience locally at their cell surface might be much higher given the proximity of the AgNPs during laser exposure.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Silver Nanoparticle-Induced Photothermal Therapy and Its Augmentation of Hyperthermia on Breast Cancer Cells Harboring Intracellular Bacteria

Liu,

Phillips,

Northrup

et al. 2023

Pharmaceutics

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Breast cancer can harbor intracellular bacteria, which may have an impact on metastasis and therapeutic responses. Silver nanoparticles are FDA-approved for their antimicrobial potential, plus they have pleiotropic benefits for eradicating cancer cells. In the current work we synthesized photothermal silver nanoparticles (AgNPs) with an absorption at 800 nm for heat generation when exposed to near-infrared laser irradiation. Breast cell lines MCF 10A, MCF7, and MDA MB 231 were infected with Pseudomonas aeruginosa, and their response to AgNPs, heat, or photothermal therapy (PTT) was evaluated. The results demonstrate that the application of a brief heating of cells treated with AgNPs offers a synergistic benefit in killing both infected and non-infected cells. Using 10 µg/mL of AgNPs plus laser stimulation induced a temperature change of 12 °C, which was sufficient for reducing non-infected breast cells by 81–94%. Infected breast cells were resistant to PTT, with only a reduction of 45–68%. In the absence of laser stimulation, 10 µg/mL of AgNPs reduced breast cell populations by 10–65% with 24 h of exposure. This concentration had no impact on the survival of planktonic bacteria with or without laser stimulation, although infected breast cells had a 42–90% reduction in intracellular bacteria. Overall, this work highlights the advantages of AgNPs for the generation of heat, and to augment the benefits of heat, in breast cancer cells harboring intracellular infection.

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“…As mentioned above, as a local therapy modality, biomaterial scaffolds with PTT is not efficient enough to ablate residual tumor cells far from the defect sites. [ 134 ] However, PDT is mainly based on the energy transfer from specific light‐activated PSs to molecular oxygen, thus generating singlet oxygen ( 1 O 2 ), which can induce tumor cells apoptosis and/or necrosis. [ 135 ] Hence, a strategy to fabricate biomaterial scaffolds with combined PTT and PDT has been proposed to enhance tumor therapeutic efficacy as well as stimulate bone regeneration.…”

Section: Biomaterials Scaffolds With Synergistic Tumor Therapy and Bonmentioning

confidence: 99%

Recent Advances in Biomaterial Scaffolds for Integrative Tumor Therapy and Bone Regeneration

Huang

Tian

Zhu

et al. 2020

Advanced Therapeutics

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Bone tumors bring great pain to patients, along with a high disability rate and high mortality. Several therapeutic strategies have been applied to treat bone tumors, such as chemotherapy, radiotherapy, and surgical resection. However, these strategies have intrinsic drawbacks, such as the serious side effects of chemotherapy or the necessary reconstructive surgery of surgical resection. To help develop more satisfactory bone tumor treatment strategies, biomaterial scaffolds have been proposed. Biomaterial scaffolds are often applied for repairing bone defects after surgical resection due to their biocompatibility, osteoinductivity, and osteoconductivity as well as excellent physicochemical properties. Simultaneously, the function of bone tumor therapy offers biomaterial scaffolds to eliminate residual tumor cells, thus avoiding tumor recurrence. This review summarizes recent advances in biomaterial scaffolds which are combined with chemotherapy or hyperthermia therapy for treating bone tumors and further bone regeneration. Moreover, the progress in synergistic therapy of bone tumors based on biomaterial scaffolds is also discussed. This review aims to inspire researchers to develop novel fabrication or functionalization strategies for constructing multifunctional biomaterial scaffolds with anti-tumor properties.

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Assessing fluorescence detection and effective photothermal therapy of near‐infrared polymer nanoparticles using alginate tissue phantoms

Cited by 10 publications

References 58 publications

Oxaliplatin-resistant colorectal cancer models for nanoparticle hyperthermia

Oxaliplatin-resistant colorectal cancer models for nanoparticle hyperthermia

The Impact of Silver Nanoparticle-Induced Photothermal Therapy and Its Augmentation of Hyperthermia on Breast Cancer Cells Harboring Intracellular Bacteria

Recent Advances in Biomaterial Scaffolds for Integrative Tumor Therapy and Bone Regeneration

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