Assessing DNA damage from enzyme-oxidized single-walled carbon nanotubes

Pan, Shenmin; Sardesai, Naimish P.; Liu, Hongyun; Li, Dandan; Rusling, James F.

doi:10.1039/c3tx50022e

Cited by 13 publications

(11 citation statements)

References 32 publications

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“…S6 †). We did not test later time-points because signicant, spontaneous cell death starts to occur in neutrophils aer 6 h. These ndings are in accordance with the recent publication by Pan et al 29 showing that HRP oxidized SWCNTs and their byproducts may yield DNA damage in vitro and in A549 lung carcinoma cells. Notably, Allen et al 11 previously identied oxidized polyaromatic hydrocarbons, well-known tumor inducing agents, following HRP-mediated degradation of SWCNTs.…”

Section: Cytotoxicity Of Partially Degraded Cntssupporting

confidence: 84%

Extracellular entrapment and degradation of single-walled carbon nanotubes

et al. 2014

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Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.

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Section: Cytotoxicity Of Partially Degraded Cntssupporting

confidence: 84%

Extracellular entrapment and degradation of single-walled carbon nanotubes

et al. 2014

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“…However, these intermediate PAHs may have important toxicological implications (Bai et al, 2014). For example, it has been demonstrated that the oxidation products (e.g., MW < 3000 Da) of horseradish peroxidase-catalyzed degradation of single-wall carbon nanotubes will induce DNA damage (Pan et al, 2013). It was suggested that the products from photoreaction of GO are likely to exhibit different accumulation and toxic properties compared to parent GO (Hou et al, 2015), but the potential toxicological impacts of FLG degraded by the Fenton reaction and its byproducts are not yet well understood.…”

Section: Daphnia Magna Resultsmentioning

confidence: 99%

Degradation of 14C-labeled few layer graphene via Fenton reaction: Reaction rates, characterization of reaction products, and potential ecological effects

et al. 2015

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“… 22 − 26 In particular, peroxidases such as horseradish peroxidase (HRP), myeloperoxidase, and eosinophil peroxidase have been demonstrated to degrade both carbon nanotubes and graphene oxide. Although the degradation products of myeloperoxidase were shown not to induce toxicity, 23 recently, the oxidation products (e.g., MW < 3000 Da) of HRP-catalyzed degradation of SWCNTs have been identified to induce DNA damage; 27 in that study, however, no structures were identified. Therefore, structural elucidation of the degradation products of carbon nanomaterials represents a relevant and fundamentally important step with implications for diverse fields.…”

Section: Introductionmentioning

confidence: 85%

Insight into the Mechanism of Graphene Oxide Degradation via the Photo-Fenton Reaction

Bai¹,

Jiang²,

Kotchey³

et al. 2014

J. Phys. Chem. C

105

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Graphene represents an attractive two-dimensional carbon-based nanomaterial that holds great promise for applications such as electronics, batteries, sensors, and composite materials. Recent work has demonstrated that carbon-based nanomaterials are degradable/biodegradable, but little work has been expended to identify products formed during the degradation process. As these products may have toxicological implications that could leach into the environment or the human body, insight into the mechanism and structural elucidation remain important as carbon-based nanomaterials become commercialized. We provide insight into a potential mechanism of graphene oxide degradation via the photo-Fenton reaction. We have determined that after 1 day of treatment intermediate oxidation products (with MW 150–1000 Da) were generated. Upon longer reaction times (i.e., days 2 and 3), these products were no longer present in high abundance, and the system was dominated by graphene quantum dots (GQDs). On the basis of FTIR, MS, and NMR data, potential structures for these oxidation products, which consist of oxidized polycyclic aromatic hydrocarbons, are proposed.

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Assessing DNA damage from enzyme-oxidized single-walled carbon nanotubes

Abstract: Peroxidase enzyme digests of oxidized single-wall carbon nanotubes (SWCNT) were shown to damage DNA in potentially genotoxic reactions for the first time using an electro-optical array with and without metabolic activation.

Cited by 13 publications

References 32 publications

Extracellular entrapment and degradation of single-walled carbon nanotubes

Extracellular entrapment and degradation of single-walled carbon nanotubes

Degradation of 14C-labeled few layer graphene via Fenton reaction: Reaction rates, characterization of reaction products, and potential ecological effects

Insight into the Mechanism of Graphene Oxide Degradation via the Photo-Fenton Reaction

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